Human papillomavirus and lung cancinogenesis: an overview

Freitas, Antônio Carlos de; Gurgel, Ana Pavla Almeida Diniz; Lima, Elyda Golçalves de; Marcos, B; Amaral, Carolina

doi:10.1007/s00432-016-2197-1

Cited by 36 publications

(41 citation statements)

References 121 publications

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“…In the framework of a molecular study, all tumours will be analysed using targeted next generation sequencing (hotspotcancer panel V2, iontorrent technology, proton sequencing system). In addition, the availability of tumour tissue could allow to detect the presence of viruses that are suspected to play a role in lung cancer development, like HPV [51]. …”

Section: Methodsmentioning

confidence: 99%

Women Epidemiology Lung Cancer (WELCA) study: reproductive, hormonal, occupational risk factors and biobank

et al. 2017

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BackgroundLung cancer aetiology and clinical aspects have been mainly studied in men, although specific risk factors probably exist in women. Here we present the rationale, design and organization of the WELCA study (Women Epidemiology Lung CAncer) that has been launched to investigate lung cancer in women, focusing particularly on hormonal and occupational factors.Methods/DesignWELCA is a population based case-control study and planned to recruit 1000 cases and 1000 controls in three years, based on study power calculation. Eligible cases are female patients newly diagnosed with lung cancer, living in Paris and the Ile de France area and aged up to 75 years. Almost all Parisian pneumology and oncology clinical departments are involved. The control group is a random sample of the population living in the same area, frequency-matched on age and additionally stratified on the distribution of socio-professional categories of women residing there. After acquisition of written consent, research nurses administer standardized computer assisted questionnaires to all the subjects in face-to-face interviews and acquire anthropometric measures. Besides usual socio-demographic characteristics, information is gathered about menstrual and reproductive factors, hormonal treatments, lifestyle and leisure characteristics, occupational history, personal and familial medical history. Biological samples are also collected, in order to establish a biobank for molecular epidemiology studies. Molecular characteristics of the tumours will be obtained and patients will be followed up for five years.DiscussionThe WELCA study aims to answer key questions in lung cancer aetiology and clinical characteristics specifically in women. The role of hormonal impregnation is investigated, and the interactions with cigarette smoking or body mass index (BMI) will be analyzed in detail. The occupational history of the subjects is carefully reconstructed, focusing in particular on the service sector. The creation of a biobank for collection of serum, plasma, DNA and tumour tissue will allow the genetic and biochemical characterization of both the subjets and the tumours. The follow-up of the patients will help in disentangling the role of hormonal factors and tumour molecular characteristics in survival.

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Section: Methodsmentioning

confidence: 99%

Women Epidemiology Lung Cancer (WELCA) study: reproductive, hormonal, occupational risk factors and biobank

et al. 2017

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“…Unlike cervical cancer, the HPV viral load in lung cancer is very low, with less than one copy per cell 26 . However, like cervical cancer, HPV frequently integrates into the host genome, typically causing E2 gene disruption 26,27 . In some studies, HPV infection may have been overlooked when the assay targeted the part of the HPV genome that is lost during integration.…”

Section: Discussionmentioning

confidence: 99%

“…We designed a qPCR assay with a very low LOD to eliminate the likelihood of false negativities. This qPCR can detect up to 4 or 40 copies of HPV DNA per reaction, and amplifies the E6 HPV gene which is necessary for HPV-related cancer development and is, therefore, present during all clinically-relevant infections 26 . DNA degradation usually occurs in FFPE samples, and these samples can produce false negative results.…”

Section: Discussionmentioning

confidence: 99%

The absence of high-risk human papillomavirus in Czech non-small cell lung cancer cases

Jaworek

Koudeláková

Slavkovský

et al. 2020

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aims. The purpose of our study was to examine the presence of human papillomavirus (HPV) DNA in Czech patients with non-small cell lung cancer (NSCLC). Methods. A highly sensitive quantitative polymerase chain reaction (qPCR) detecting the E6 gene of HPV16, 18, 31, and 56 was designed. The limit of detection was assessed using serial dilutions of HPV-positive plasmids. The qPCR was validated on a set of 402 cervical swabs where the qPCR, Cobas, and PapilloCheck methods were tested in parallel. Finally, qPCR was used for HPV detection in a set of 80 patients with primary NSCLC, both from formalin-fixed paraffinembedded (FFPE) and fresh frozen (FF) tissue samples. Results. The qPCR method was able to reliably detect at least 4 copies of the E6 gene per reaction in HPV16, 18, and 31, and 40 copies per reaction in HPV56. The sensitivity and specificity of the qPCR were 75.6-99.3% and 63.9-100% respectively, depending on the HPV genotype and reference method used. HPV DNA was not detected in the FFPE and FF samples from the set of 80 NSCLC patients. Conclusion. No hrHPV DNA was found in primary NSCLC tumors from a Czech population.

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“…Although much research effort has been made in improving treatment for LC in recent decades, a survival rate of five years is still less than 20% [5]. There are two main pathological types of LC: small cell lung cancer (SCLC) accounting for about 85% and non-small cell lung cancer (NSCLC) accounting for about 13%-15% [6]. The main symptoms of LC are coughing, hemoptysis, dyspnea, and fatigue.…”

Section: Introductionmentioning

confidence: 99%

Network Pharmacology Approach Reveals the Potential Immune Function Activation and Tumor Cell Apoptosis Promotion of Xia Qi Decoction in Lung Cancer

Zhang

Wang

2019

Medical Sciences

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As the leading cause of cancer death worldwide, lung cancer (LC) has seriously affected human health and longevity. Chinese medicine is a complex system guided by traditional Chinese medicine theories (TCM). Nowadays, the clinical application of TCM for LC patients has become the focus for its effectiveness and security. In this paper, we will analyze and study the mechanism of Xia Qi Decoction (XQD) in the treatment of LC. The results collectively show that XQD could act on 41 therapeutic targets of LC. At the same time, 8 of 41 targets were significantly expressed in immune tissues and cells by activating CD8+T cells to promote apoptosis of cancer cells. It reveals the molecular mechanism of XQD in the treatment of LC from the perspective of network pharmacology. In addition, in the treatment of LC, XQD can activate (up-regulate) the function of immune cells, promote the apoptosis of tumor cells, and have an active anti-tumor immune effect. In conclusion, this study reveals the unique advantages of traditional Chinese medicine in the treatment of cancer, in reinforcing the healthy qi and eliminating the pathogenic factors. More research, however, is needed to verify the potential mechanisms.

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Human papillomavirus and lung cancinogenesis: an overview

Cited by 36 publications

References 121 publications

Women Epidemiology Lung Cancer (WELCA) study: reproductive, hormonal, occupational risk factors and biobank

Women Epidemiology Lung Cancer (WELCA) study: reproductive, hormonal, occupational risk factors and biobank

The absence of high-risk human papillomavirus in Czech non-small cell lung cancer cases

Network Pharmacology Approach Reveals the Potential Immune Function Activation and Tumor Cell Apoptosis Promotion of Xia Qi Decoction in Lung Cancer

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