Human Papillomavirus Detection from Human Immunodeficiency Virus-Infected Colombian Women's Paired Urine and Cervical Samples

Abstract

Infection, coinfection and type-specific human papillomavirus (HPV) distribution was evaluated in human immunodeficiency virus (HIV)-positive women from paired cervical and urine samples. Paired cervical and urine samples (n = 204) were taken from HIV-positive women for identifying HPV-DNA presence by using polymerase chain reaction (PCR) with three generic primer sets (GP5+/6+, MY09/11 and pU1M/2R). HPV-positive samples were typed for six high-risk HPV (HR-HPV) (HPV-16, -18, -31, -33, -45 and -58) and two low-risk (LR-HPV) (HPV-6/11) types. Agreement between paired sample results and diagnostic performance was evaluated. HPV infection prevalence was 70.6% in cervical and 63.2% in urine samples. HPV-16 was the most prevalent HPV type in both types of sample (66.7% in cervical samples and 62.0% in urine) followed by HPV-31(47.2%) in cervical samples and HPV-58 (35.7%) in urine samples. There was 55.4% coinfection (infection by more than one type of HPV) in cervical samples and 40.2% in urine samples. Abnormal Papanicolau smears were observed in 25.3% of the women, presenting significant association with HPV-DNA being identified in urine samples. There was poor agreement of cervical and urine sample results in generic and type-specific detection of HPV. Urine samples provided the best diagnosis when taking cytological findings as reference. In conclusion including urine samples could be a good strategy for ensuring adherence to screening programs aimed at reducing the impact of cervical cancer, since this sample is easy to obtain and showed good diagnostic performance.

Comparison of human papillomavirus (HPV) detection in urine and cervical swab samples using the HPV GenoArray Diagnostic assay

“…Under these circumstances, testing urine for HPV DNA offers a distinct advantage (Prusty et al, 2005). Previous studies have compared HPV detection rates between cervical and urine samples in order to evaluate the ability of urine-based assays to detect the prevalence of HPV independently of cervical cytology assays (Daponte et al, 2006; Munoz et al, 2013). Evidence suggests that the sensitivity of urine testing for HPV 16 and 18 was higher for participants with cervical cancer (88.8%) than for those with high- and low-grade lesions (Daponte et al, 2006).…”

Human papillomavirus genotype distribution among Cameroonian women with invasive cervical cancer: a retrospective study

“…What is worth noting is that the majority of HPV45 was present in co-infections with HPV16. There are substantial data suggesting that in HIV-positive women there is higher prevalence of HPV and more frequent multiple type co-infections, and one small study in Cameroon comparing HPV infections and HIV status showed HPV45 to be the dominant co-infection type 15 16. More studies are required to address the association between HIV infection and the prevalence of HPV types in women with normal cytology or with ICC.…”

Comparison of Detection Sensitivity for Human Papillomavirus between Self-collected Vaginal Swabs and Physician-collected Cervical Swabs by Electrochemical DNA Chip

“…It was different from a previous survey in Thai women, which reported HPV16 as the most common genotype identified (17.9%) (Chansaenroj et al, 2010). HPV 16 remained the most prevalent HPV genotype in Thailand as well as in many other countries (Onuki et al, 2009;Bissett et al, 2011;Munoz et al, 2013).…”