Human papillomavirus infection and its association with cervical dysplasia in Ecuadorian women attending a private cancer screening clinic

Brown, C.R.; León, Marielisa; Muñoz, Kathia Valverde; Fagioni, A.; Amador, L.G.; Frain, Barbara McGahey; Tu, Wanzhu; Qadadri, Brahim; Brown, Darron R.

doi:10.1590/s0100-879x2009000700007

Cited by 29 publications

(34 citation statements)

References 29 publications

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“…Studies in Ecuador found genotype 16 as the most common [32, 33, 35, 40], along with results of this investigation. The 18 genotype was not reported as the second most frequent, suggesting that genotypic variability by geographic region and sociodemographic factors had an effect, such that more research is necessary.…”

Section: Discussionsupporting

confidence: 80%

“…In the city of Cuenca, in a study of 500 women from the general population, the prevalence of 25.6% of HPV for both high and low risk [34] was similar to that reported in Santa Elena. Brown et al (2009) found [35] a prevalence of 24.2%. A higher prevalence of HPV with abnormal cytology studies indicates a relationship between the virus and malignant cells.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence of High-Risk Genotypes of Human Papillomavirus: Women Diagnosed with Premalignant and Malignant Pap Smear Tests in Southern Ecuador

Aguilar

González

Rodriguez

et al. 2017

Infectious Diseases in Obstetrics and Gynecology

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Human papillomavirus (HPV) is the primary infectious agent for the development of cervical cancer, although the presence of the virus alone is insufficient for viral development and proliferation; this can be attributed to the increase in potential oncogenic risk, along with other risk factors. In the present investigation, the prevalence of high-risk HPV was determined from samples of premalignant or malignant cervical cytology in women from the southern region of Ecuador. The kit we used was able to detect genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, and 59. In addition, 64.5% of the analyzed samples were positive for HPV, with genotypes 16 and 18 being the most prevalent (16 was detected in 148 samples and 18 in 108). Genotypes 58 and 51 were the third most frequent simple and multiple infections, respectively. The data are very similar to those obtained worldwide, suggesting that the strategy of sex education, and the use of vaccines as primary prevention agents, could significantly decrease the incidence and mortality rate of cervical cancer in the southern region of Ecuador.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Prevalence of High-Risk Genotypes of Human Papillomavirus: Women Diagnosed with Premalignant and Malignant Pap Smear Tests in Southern Ecuador

Aguilar

González

Rodriguez

et al. 2017

Infectious Diseases in Obstetrics and Gynecology

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“…HPV detection and genotyping was performed using the Linear Array HPV Genotyping Test (LA-HPV) (Roche, Indianapolis, Indiana) as described previously [9-12]. The GH20/PC04 human β-globin target was co-amplified to determine sample adequacy.…”

Section: Methodsmentioning

confidence: 99%

Invasive cervical cancers from women living in the United States or Botswana: differences in human papillomavirus type distribution

Ermel

Ramogola‐Masire

Zetola

et al. 2014

Infect Agents Cancer

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BackgroundCervical cancer is the primary cause of cancer-related deaths in women living in Botswana.MethodsParaffin-embedded blocks of formalin-fixed invasive cervical cancer specimens were identified from women living in the U.S. (n = 50) or Botswana (n = 171) from which DNA was extracted. Thin-section PCR was performed on each sample for HPV types and HIV. Comparisons were made between HPV types and groups of types identified in cancers.ResultsHPV DNA was identified in 92.0% of specimens from the U.S. containing amplifiable human DNA, and 79.5% of specimens from Botswana. HPV 16 was detected in 40 of 46 HPV-positive specimens (87.0%) from the U.S. vs. 58 of 136 (42.7%) from Botswana (p < 0.001). In contrast, non-HPV 16/18 types, all A9 species (HPV16, 31, 33, 35, 52, and 58), non-HPV 16 A9 (HPV 31, 33, 35, 52, and 58), HPV 18, all A7 types (18, 39, 45, 59, and 68) types were detected significantly more often in specimens from Botswana. The prevalence of non-HPV 18 A7 types did not differ significantly between the two groups. For specimens from Botswana, 31.6% contained PCR-amplifiable HIV sequences, compared to 3.9% in U.S. specimens. Stratifying the samples from Botswana by HIV status, HPV 31 was detected significantly more often in HIV-positive specimens. Other HPV types and groups of types were not significantly different between HIV-positive and HIV-negative specimens from Botswana.ConclusionThis study demonstrates that there may be important HPV type differences in invasive cervical cancers occurring in women living in the United States or Botswana. Factors in addition to HIV may be driving these differences.

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“…Samples were analyzed for specific HPV types using LA-HPV as previously described [Gravitt et al, 2000;Brown et al, 2005Brown et al, , 2009Shew et al, 2006;Fife et al, 2009]. A participant was considered HPV 16-positive if at least one of their samples contained HPV 16 DNA when tested with LA-HPV.…”

Section: Sample Collection and Hpv Genotypingmentioning

confidence: 99%

Low‐level persistence of human papillomavirus 16 DNA in a cohort of closely followed adolescent women

Weaver

Shew

Qadadri

et al. 2011

Journal of Medical Virology

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Most human papillomavirus (HPV) infections in young women become undetectable by standard assays after a few months. It is possible that many HPV infections do not actually clear, but persist at very low levels for years, becoming detected again later in life. The purpose of this study is to describe HPV 16 clearance, reappearance, and low-level persistence in a cohort of adolescent women. Adolescent women (N = 66), not vaccinated against HPV, were recruited from 1998 to 2008 into a longitudinal study. Self-collected vaginal samples were obtained quarterly and tested for HPV by Linear Array HPV Genotyping Test (LA-HPV). To explore low-level persistence, a type-specific nested PCR for HPV 16 (TSN-PCR-16) was developed. Women with HPV 16 detected by LA-HPV had their negative swabs retested with TSN-PCR-16. Forty-two participants with HPV 16, followed for a mean of 6.3 years, were analyzed. Using LA-HPV, the median duration of HPV 16 detection was 428 days (SD 852.5 days). TSN-PCR-16 detected HPV 16 during periods of LA-HPV non-detection in samples from many women. Using a combination of LA-HPV and TSN-PCR-16 results, the median duration of HPV 16 detection was 1,022.5 days (SD 943.7 days). The durations of detection differed significantly between the two methods (P = 0.0042) with a mean difference of 434.5 days. In adolescent females, duration of HPV 16 detection was significantly longer when TSN-PCR-16 was combined with LA-HPV. Some apparently cleared HPV 16 could be shown to persist at low levels using nested PCR.

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Human papillomavirus infection and its association with cervical dysplasia in Ecuadorian women attending a private cancer screening clinic

Cited by 29 publications

References 29 publications

Prevalence of High-Risk Genotypes of Human Papillomavirus: Women Diagnosed with Premalignant and Malignant Pap Smear Tests in Southern Ecuador

Prevalence of High-Risk Genotypes of Human Papillomavirus: Women Diagnosed with Premalignant and Malignant Pap Smear Tests in Southern Ecuador

Invasive cervical cancers from women living in the United States or Botswana: differences in human papillomavirus type distribution

Low‐level persistence of human papillomavirus 16 DNA in a cohort of closely followed adolescent women

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