Human Papillomavirus negative but dyskaryotic cervical cytology: Re-analysis of molecular testing

Peevor, Richard; Bowden, Sarah; Jones, Joanne; Fiander, Alison; Hibbitts, Samantha Jayne

doi:10.1016/j.jcv.2009.01.015

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…This rate is higher than found by Guo et al [ 58 ], whom reported that 13.6 % of their HSIL cases displayed a negative HPV-DNA molecular test. False-negative results may be due to a low burden of HPV DNA, presence of a new type of HPV not targeted by the method used, inadequate sampling, cytological and histopathological missclassification or might have been the result of methodological or reproducibility errors [ 59 , 60 ]. The fact that the E1 region, targeted by the Papillocheck assay, is located next to the E2 region in the HPV genome, could render it more vulnerable to partial deletion, that often occurs during the HPV DNA integration into the host cell DNA [ 36 ] verified on more advanced squamous intraepithelial lesions.…”

Section: Discussionmentioning

confidence: 99%

HPV genotype distribution in Brazilian women with and without cervical lesions: correlation to cytological data

Martins

Oliveira

Rosa

et al. 2016

Virol J

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BackgroundHuman Papillomavirus (HPV) genotype distribution varies according to the method of assessment and population groups. This study analyzed type-specific HPV infections among women ranging from 14–95 years old, displaying normal and abnormal cytology, from São Paulo and Barretos cities, Brazil.MethodsWomen found positive for High Risk-HPVs DNA by either the Hybrid Capture 2 (HC2) or Cobas HPV Test (n = 431) plus a random sample of 223 negative by both assays and 11 samples with indeterminate results, totalizing 665 samples, were submitted to HPV detection by the PapilloCheck test. Cytological distribution included 499 women with a cytological result of Negative for Intraepithelial Lesion or Malignancy and 166 with some abnormality as follows: 54 Atypical Squamous Cells of Undetermined Significance; 66 Low-Grade Squamous Intraepithelial Lesion; 43 High-Grade Squamous Intraepithelial Lesion and 3 (0.5 %) Invasive Cervical Cancer.ResultsFrom the 323 samples (48.6 %) that had detectable HPV-DNA by the PapilloCheck assay, 31 were HPV negative by the cobas HPV and HC2 assays. Out of these 31 samples, 14 were associated with HR-HPVs types while the remaining 17 harbored exclusively low-risk HPVs. In contrast, 49 samples positive by cobas HPV and HC 2 methods tested negative by the PapilloCheck assay (19.8 %). Overall, the most frequent HR-HPV type was HPV 16 (23.2 %), followed by 56 (21.0 %), 52 (8.7 %) and 31 (7.7 %) and the most frequent LR-HPV type was HPV 42 (12.1 %) followed by 6 (6.2 %). Among the HR-HPV types, HPV 56 and 16 were the most frequent types in NILM, found in 19.1 and 17.7 % of the patients respectively while in HSIL and ICC cases, HPV 16 was the predominant type, detected in 37.2 and 66.7 % of these samples.ConclusionsIn the population studied, HPV 16 and 56 were the most frequently detected HR-HPV types. HPV 56 was found mainly in LSIL and NILM suggesting a low oncogenic potential. HPV 16 continues to be the most prevalent type in high-grade lesions whereas HPV 18 was found in a low frequency both in NILM and abnormal smears. Surveillance of HPV infections by molecular methods is an important tool for the development and improvement of prevention strategies.

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Section: Discussionmentioning

confidence: 99%

HPV genotype distribution in Brazilian women with and without cervical lesions: correlation to cytological data

Martins

Oliveira

Rosa

et al. 2016

Virol J

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“…As it is mentioned above, 12 women with a previous HPV infection were found in the HPV-negative subgroup. This is partially explained by the results of two studies showing that a single negative HPV test result could lead to missed HPV infection [44] and that HPV test has low specificity [45]. In addition, the number of the participants as well as the age range in our study sample was relatively limited; only women aged 16-45 years were included.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of HPV infection among Greek women attending a gynecological outpatient clinic

et al. 2010

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BackgroundHuman papillomavirus (HPV) infection is a causative factor for cervical cancer. Early detection of high risk HPV types might help to identify women at high risk of cervical cancer. The aim of the present study was to examine the HPV prevalence and distribution in cervical smears in a sample of Greek women attending a gynecological outpatient clinic and to explore the determinants of the infection.MethodsA total of 225 women were studied. All women underwent a regular gynecological control. 35 HPV types were studied; 6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 56, 58, 59, 61, 62, 66, 68, 70, 71, 72, 73, 81, 82, 83, 84, 85 and 89. Also, basic demographic information, sociodemographic characteristics and sexual behavior were recorded.ResultsHPV was detected in 22.7% of the study population. The percentage of the newly diagnosed women with HPV infection was 17.3%. HPV-16 was the most common type detected (5.3%) followed by HPV-53 (4.9%). 66.2% of the study participants had a Pap test during the last year without any abnormalities. HPV infection was related positively with alcohol consumption (OR: 2.19, 95% CI: 1.04-4.63, P = 0.04) and number of sexual partners (OR: 2.16, 95% CI: 1.44-3.25, P < 0.001), and negatively with age (OR: 0.93, 95% CI: 0.87-0.99, P = 0.03), and monthly income (OR: 0.63, 95% CI: 0.44-0.89, P = 0.01).ConclusionThe prevalence of HPV in women attending an outpatient clinic is high. Number of sexual partners and alcohol consumption were the most significant risk factors for HPV infection, followed by young age and lower income.

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“…Women with positive cytology were also HPV positive in most cases. Peever et al [16] studied samples negative for HPV DNA but with dyscaryotic cells, and on re-evaluation with 3 different methods found that HPV detection was significantly higher using microarrays. Other studies also found microarrays to provide a high throughput and accurate typing [17][18][19][20].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of HPV infection by cytologic diagnosis and HPV DNA extraction and prevalence of the HPV genotypes detected in urban Turkish women

Eren

Erenus

Bas

et al. 2010

International Journal of Gynecology & Obstetrics

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Human Papillomavirus negative but dyskaryotic cervical cytology: Re-analysis of molecular testing

Cited by 6 publications

References 12 publications

HPV genotype distribution in Brazilian women with and without cervical lesions: correlation to cytological data

HPV genotype distribution in Brazilian women with and without cervical lesions: correlation to cytological data

Prevalence of HPV infection among Greek women attending a gynecological outpatient clinic

Prevalence of HPV infection by cytologic diagnosis and HPV DNA extraction and prevalence of the HPV genotypes detected in urban Turkish women

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