Human Papillomavirus Testing for Triage in a Referral Population

Gogola, Jon; Dinh, Tung Van; Lucci, Joseph A.; Smith, Edward; Hannigan, Edward V.

doi:10.1046/j.1526-0976.2001.51006.x

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…According to the manufacturer, Qiagen, “The digene HC2 HPV DNA Test is an in vitro nucleic acid hybridization assay with signal amplification using microplate chemiluminescence for the qualitative detection of 18 types of HPV DNA in cervical specimens. The digene HC2 HPV DNA Test can differentiate between two HPV DNA groups, low‐ and high‐risk types [68].” The HPV tests on the market only help doctors screen patients for the presence of a group of HPVs that may cause cellular changes, which may lead to cervical cancer in some patients if not treated. These commercial test kits are designed to screen 13 “high‐risk” HPV genotypes out of about 40 genotypes commonly found in the anogenital regions of women and men.…”

Section: Introductionmentioning

confidence: 99%

Is Oral Sex Really a Dangerous Carcinogen? Let’s Take a Closer Look

Rosenquist¹

2012

The Journal of Sexual Medicine

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Introduction Questions have recently arisen in the popular press about the association between specific sexual behaviors, namely, fellatio and cunnilingus, with head and neck cancers. Although there has been an overall decline in the incidence of head and neck cancers over the past 25 years, there has been a shift in the distribution of these cancers toward a particular type known as oral squamous cell carcinomas (OSCCs), and a younger demographic. These particular cancers, OSCCs, have been shown to be associated with the human papillomavirus (HPV). Several researchers have suggested that this shift in the epidemiology of head and neck cancers might be attributable to changing sexual practices. While this speculation has caught on in the popular press, there are several interesting contradictions in the existing evidence that suggest this conclusion might be premature and overreached. Aim The intent of this article is to help clarify the issues so that sexual medicine professionals can give accurate and up-to-date information to their patients. Main Outcome Measures This is a review article; no outcome data are reported. This is a review article; no measures were collected. Methods Pubmed search on HPV, oral sex, oral cancers, and OSCCs. Results One hundred ninety-six articles on HPV were found; 63 articles on oral sex, 55 on oral cancer, and 5 articles on OSCCs were identified as relevant. Conclusions HPV infections occur commonly and are usually cleared within 18 months, thus HPV infection should not be a cause for concern among monogamous couples with a rich and varied sex life as long as the sexual system remains closed and other immune compromising factors are not present. HPV becomes a concern in the context of immune system compromise and infection persistence. Factors contributing to immune system compromise, HPV persistence, and oncogenesis are reviewed.

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Section: Introductionmentioning

confidence: 99%

Is Oral Sex Really a Dangerous Carcinogen? Let’s Take a Closer Look

Rosenquist¹

2012

The Journal of Sexual Medicine

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“…Digene Hybrid Capture 2 (HC2) test, the only test approved by the U.S. Food and Drug Administration (FDA), is commonly used to determine if a cervicovaginal cell suspension contains "high-risk" oncogenic HPVs [2], often functioning as a triage for colposcopic evaluation of the cytologically borderline cases [3-5]. However, it is now recognized that persistent infection of a "high-risk" HPV, not the mere presence of the HPV virus itself, is the pivotal promoter in causing cervical precancerous lesions and cancer [6-9].…”

Section: Introductionmentioning

confidence: 99%

Routine human papillomavirus genotyping by DNA sequencing in community hospital laboratories

Lee

Vigliotti

et al. 2007

Infect Agents Cancer

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Background: Human papillomavirus (HPV) genotyping is important for following up patients with persistent HPV infection and for evaluation of prevention strategy for the individual patients to be immunized with type-specific HPV vaccines. The aim of this study was to optimize a robust "lowtemperature" (LoTemp™) PCR system to streamline the research protocols for HPV DNA nested PCR-amplification followed by genotyping with direct DNA sequencing. The protocol optimization facilitates transferring this molecular technology into clinical laboratory practice. In particular, lowering the temperature by 10°C at each step of thermocycling during in vitro DNA amplification yields more homogeneous PCR products. With this protocol, template purification before enzymatic cycle primer extensions is no longer necessary.

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Human Papillomavirus Testing for Triage in a Referral Population

Abstract: Although HPV high-risk positive results correlate with high-grade histology, it is associated with significant false negatives. The HPV low-risk test is not clinically useful. These tests were poor methods to triage patients in our population.

Cited by 2 publications

References 17 publications

Is Oral Sex Really a Dangerous Carcinogen? Let’s Take a Closer Look

Is Oral Sex Really a Dangerous Carcinogen? Let’s Take a Closer Look

Routine human papillomavirus genotyping by DNA sequencing in community hospital laboratories

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