Human Papillomavirus‐type distribution in anogenital lesions of prepubertal children

Abstract

Background In contrast to adults, only limited data are available on the human papillomavirus (HPV)-type spectrum in anogenital warts (AGW) of children.Objective This study aimed to evaluate the HPV-type spectrum in AGW of prepubertal children. Materials & methodsIn a retrospective German multicentre study, HPV genotyping was performed in AGW biopsies of 55 1-to 12-year-old children using HPV group-specific PCRs followed by hybridization with type-specific probes or sequence analysis.Results Human papillomavirus-DNA was found in 53 of the 55 AGW. In 58.5% (31/53) of the HPV-positive AGW, mucosal HPV types were detected. HPV6 (27/53, 50.9%) was the predominant type. 43.4% (23/53) of the lesions were induced by cutaneous HPV types (HPV2, HPV27, HPV57). Mucosal HPV types were significantly more common in children under 5 years of age than in children 5 years of age and older (22/25, 88.0% [95% CI: 70.0-95.8] vs. 9/28, 32.1% [95% CI: 17.9-50.7], P < 0.001). In contrast, cutaneous HPV types were significantly more prevalent in the 5-to 12-year age group (4/25,.1], P < 0.001). ConclusionAnogenital warts in 5-to 12-year-old children are frequently associated with cutaneous HPV types, possibly due to horizontal transmission. HPV typing, in addition to comprehensive clinical and psychosocial evaluation, can potentially help in the assessment of these cases.

Immunothrombotic Mechanisms Induced by Ingenol Mebutate Lead to Rapid Necrosis and Clearance of Anogenital Warts

“…The warts were immediately fixed in 4% formalin solution and in an optimal cutting temperature (OCT) compound (Sakura Finetek USA, USA). HPV infection with subtype was confirmed on paraffin embedded warts (t0) by short-fragment group-specific HPV-PCR followed by HPV-typing with a reverse line-blot assay that covers 32 alpha-HPV types (INNO-LiPA HPV Genotyping Extra II, Fujirebio, Gent, Belgium), as previously described in more detail [ 55 ].…”

Kutane Infektionen im Kindesalter

“…Das ist bei der Aufklärung der Eltern zu bedenken [11]. In einer retrospektiven Untersuchung an Paraffinblöcken von 53 Kindern mit Condylomata acuminata wurde bei über der Hälfte der Kinder molekulargenetisch HPV 11 festgestellt [19].…”

Kutane Infektionen im Kindesalter

“…Das ist bei der Aufklärung der Eltern zu bedenken 11 . In einer retrospektiven Untersuchung an Paraffinblöcken von 53 Kindern mit Condylomata acuminata wurde bei über der Hälfte der Kinder molekulargenetisch HPV 11 festgestellt 19 .…”