Human papillomavirus types 11 and 16 detected in nasopharyngeal carcinomas by the polymerase chain reaction

Hørding, Ulla; Nielsen, Hans Bruun; Daugaard, Søren; Albeck, Henrik

doi:10.1288/00005537-199401000-00018

Cited by 53 publications

(43 citation statements)

References 26 publications

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“…Fourteen of the 32 (43.5%) specimens were positive for HPV DNA with the E1 primers, whereas 12 of the 32 (37.5%) were positive with the L1 primers ( Table 2). Three samples (patients 6,28,29) were positive with the E1 primers only, and 1 (patient 1) was positive with the L1 primers only. The concordance between the two primer sets was 87.5%.…”

Section: Resultsmentioning

confidence: 98%

Human papillomavirus infection in ?young? versus ?old? patients with squamous cell carcinoma of the head and neck

et al. 2000

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Background. Human papillomavirus (HPV) represents a potential risk factor for squamous cell cancer of the head and neck (SCCHN). We evaluated the prevalence of HPV DNA in patients with SCCHN diagnosed at the University of Michigan from 1994-1996. Methods. Patients were stratified by age at diagnosis as "young" (<50 years; median, 39) or "old" (>50 years; median, 66). Fourteen "young" and 14 "old" were matched for tumor site, and 4 additional "old" patients were included. Specimens were analyzed by polymerase chain reaction for HPV DNA using 2 sets of consensus primers. HPV sequences were confirmed by Southern blot hybridization and typed with type-specific probes.Results. Overall, 15 of 32 (46.9%) samples contained HPV sequences. HPV 16 was detected in 9 of 15 (60%), HPV-18 in 1 of 15 (6.6%), and 5 of 15 (33.3%) remained untyped by multiple methods. When stratified, 7 of 14 (50%) "young" were HPVpositive compared with 8 of 18 (44.4%) "old" (p = .76). Survival in patients with HPV-positive SCCHN was significantly longer than that for HPV-negative patients.Conclusions. The incidence of HPV in "young" versus "old" is not significantly different, suggesting similar roles for both groups. Patients with HPV-positive tumors may have a survival advantage relative to patients with HPV-negative tumors.

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Section: Resultsmentioning

confidence: 98%

Human papillomavirus infection in ?young? versus ?old? patients with squamous cell carcinoma of the head and neck

et al. 2000

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“…HPV DNA has been identified in primary tumors of the tonsils [Snijders et al, 1992;Paz et al, 1997], larynx, hypopharynx [Clayman et al, 1994], oral cavity [Kashima et al, 1990], tongue [De Villers et al, 1985], nasopharynx [Hording et al, 1994], in cell lines derived from a variety of head and neck carcinomas [Bradford et al, 1991], and in inverted papillomas that have progressed to squamous cell carcinoma [Beck et al, 1995]. Precancerous lesions [Fouret et al, 1995] and metastatic lymph nodes [Miller and White, 1996;Paz et al, 1997] have also been shown to contain DNA of the same HPV type as the primary tumor, supporting the involvement of HPV in the development of squamous cell carcinoma.…”

Section: Introductionmentioning

confidence: 99%

The prevalence of human papillomavirus in the oropharynx in healthy individuals in a Brazilian population

et al. 2006

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Oncogenic human papillomavirus (HPV), a causative agent of uterine cervical cancer, has also been detected in head and neck squamous cell cancers, especially in squamous cell carcinomas of the tonsils. However, the true HPV prevalence in normal and neoplasic oropharyngeal mucosa remains uncertain. To determine the prevalence of HPV DNA in normal oropharyngeal mucosa of cancer-free individuals, a study was carried out on 50 Brazilian subjects. PCR was performed to identify HPV DNA in samples from four sites in the oropharynx (tonsils, soft palate, base of the tongue, and back wall of the pharynx). For amplification of the HPV DNA, MY09/11 consensus primers were used, and specific genotypes were identified by dot-blot hybridization or cloning and sequencing. HPV DNA was present in 14.0% of the individuals, and the identified genotypes were 16, 18, 52, and 61. All these types are considered high-risk (HR) HPV. The tonsils and the soft palate were the sites with the highest HPV prevalence. This study shows the prevalence of HR HPV in the oropharynx of normal individuals. However, the prevalence of HPV is still unclear, and if HPV infection in a healthy it is not known individual predisposes to HPV-associated disease such as oropharyngeal cancer. Thus, it is important to assess the prevalence of HPV in cancer-free individuals, in order to compare it with the HPV prevalence in oropharyngeal carcinomas and to attempt to determine the true role of HPV in the development of head and neck squamous cell cancers.

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“…Schwartz et al 3 and Haraf et al 4 also found a significant predilection for the tonsillar site. HPV has also been detected in nasopharyngeal carcinomas, 5 in cell lines derived from a variety of head and neck carcinomas, 6 and in inverted papillomas that have progressed to SCC. 7 Precancerous lesions 8,9 and metastatic lymph nodes 10,11 have also been shown to contain DNA of the same HPV type as the primary tumor, supporting the involvement of HPV in the development of SCC.…”

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confidence: 99%

Human papillomavirus and p53 mutational status as prognostic factors in head and neck carcinoma

et al. 2002

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Human papillomavirus types 11 and 16 detected in nasopharyngeal carcinomas by the polymerase chain reaction

Cited by 53 publications

References 26 publications

Human papillomavirus infection in ?young? versus ?old? patients with squamous cell carcinoma of the head and neck

Human papillomavirus infection in ?young? versus ?old? patients with squamous cell carcinoma of the head and neck

The prevalence of human papillomavirus in the oropharynx in healthy individuals in a Brazilian population

Human papillomavirus and p53 mutational status as prognostic factors in head and neck carcinoma

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