Human papillomaviruses 16 and 58 are distributed widely among women living in Shanghai, China, with high-grade, squamous intraepithelial lesions

Abstract

The distribution of human papillomaviruses (HPVs) must be understood for the control and prevention of cervical cancer. Community-based Papanicolaou and HPV DNA tests were performed on 41 578 women. The prevalences of HPV genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 were assessed. In total, 10% women were infected/co-infected by these HPVs. The infection rate increased from 7.1% in women aged ⩽30 years to 10.4% in those aged 50–60 years, and then decreased slightly to 9.9% in those aged >60 years. The HPV 16 and 58 positivity rates were significantly higher among women with high-grade squamous intraepithelial lesions (HSILs) than among those with cervicitis/negativity for intraepithelial lesion or malignancy (NILM) or low-grade SILs (LSILs). The HPV 18, 52 and 68 infection rates were significantly lower in women with HSILs than in those with NILM or LSILs. The proportion of women infected by multiple HPV strains was higher among those with HSILs. The proportions of the five most common genotypes, HPV 16, 18, 33, 52 and 58, increased with the number of co-infecting strains. HPV 16 and 58 were the high-risk HPVs in the Shanghai community and should be the focus in HPV screening and vaccination.

Prevalence and type distribution of human papillomavirus in a Chinese urban population between 2014 and 2018: a retrospective study

“…Over the five-year period, HPV52 was the most dominant type among patients with positive detections, supporting the findings of several other studies (Baloch et al, 2017;Hong et al, 2015). It is followed by HPV58 and HPV16 as the most common types in female residents in Shanghai (Xu et al, 2018). In addition to single infections caused by HPV types, HPV52 also accounted for over 80% of coinfection cases, followed by HPV58 and HPV16.…”

The Prevalence of Thrice, Twice, and Once Human Papillomavirus DNA Positivity in Older Chinese Women

“…This is a retrospective study focused on older women living in the Minhang district of Shanghai. As we reported previously ( 2 ), the Shanghai Minhang District Health and Family Planning Commission have implemented a community-based Pap smear and HPV cotesting screening program in the Minhang district, which has a population of 2.43 million in nine towns. Commencing in 2014, screening was implemented in four towns with more than 50,000 women screened annually, some of whom took part in multiple annual screenings from 2014 to 2019.…”

“…As we reported previously ( 2 ), all cytological examination procedures strictly followed the 2014 Bethesda System for Reporting Cervical Cytology ( 13 ). Each cervical sample for cytological examination was taken using a sterile cytobrush inserted into the cervical os and rotated 360°.…”

“…As we reported previously ( 2 ), at the time of Pap testing, cervical specimens for HPV testing were collected using a sterile cytobrush as described above, and viral DNA was extracted using QIAamp DNA Mini Kits (QIAGEN, Shanghai, China). HPV DNA evaluation and genotyping were performed using a kit provided by Huada Biotech Co., Ltd. (Wuhan, China).…”

Genotyping of Human Papillomavirus in Cervical Squamous Intraepithelial Lesions in Mexican Women

“…Viruses belonging to the alpha-papillomavirus group infect mucosal surfaces and are found in the cervix. This genus contains 15 species (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15), of which species 7 and 9 contain the genotypes HPV-18 and HPV-16, respectively. In particular, genotypes of species 9 are associated with the development of malignant lesions (1).…”