Human pathogen co-occurrence in <i>Ixodes ricinus</i> ticks: effects of landscape topography, climatic factors and microbiota interactions

Aivelo, Tuomas; Norberg, Anna; Tschirren, Barbara

doi:10.1101/559245

Cited by 9 publications

(12 citation statements)

References 99 publications

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“…In this longitudinal three-year study, two TBP associations were significantly overrepresented compared to a random distribution: the first one was between B. garinii and B. afzelii , as has been previously observed in studies using similar detection tools [22, 42], or different methods ( 16S rRNA gene sequencing [64]); the second one was between B. garinii and B. spielmanii . Interestingly, these findings contrast with published results on I. ricinus TBPs.…”

Section: Discussionsupporting

confidence: 67%

A three-years assessment of Ixodes ricinus-borne pathogens in a French peri-urban forest

et al. 2019

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BackgroundIxodes ricinus is the predominant tick species in Europe and the primary pathogen vector for both humans and animals. These ticks are frequently involved in the transmission of Borrelia burgdorferi (sensu lato), the causative agents of Lyme borreliosis. While much more is known about I. ricinus tick-borne pathogen composition, information about temporal tick-borne pathogen patterns remain scarce. These data are crucial for predicting seasonal/annual patterns which could improve understanding and prevent tick-borne diseases.MethodsWe examined tick-borne pathogen (TBP) dynamics in I. ricinus collected monthly in a peri-urban forest over three consecutive years. In total, 998 nymphs were screened for 31 pathogenic species using high-throughput microfluidic real-time PCR.ResultsWe detected DNA from Anaplasma phagocytophilum (5.3%), Rickettsia helvetica (4.5%), Borrelia burgdorferi (s.l.) (3.7%), Borrelia miyamotoi (1.2%), Babesia venatorum (1.5%) and Rickettsia felis (0.1%). Among all analysed ticks, 15.9% were infected by at least one of these microorganisms, and 1.3% were co-infected. Co-infections with B. afzeli/B. garinii and B. garinii/B. spielmanii were significantly over-represented. Moreover, significant variations in seasonal and/or inter-annual prevalence were observed for several pathogens (R. helvetica, B. burgdorferi (s.l.), B. miyamotoi and A. phagocytophilum).ConclusionsAnalysing TBP prevalence in monthly sampled tick over three years allowed us to assess seasonal and inter-annual fluctuations of the prevalence of TBPs known to circulate in the sampled area, but also to detect less common species. All these data emphasize that sporadic tick samplings are not sufficient to determine TBP prevalence and that regular monitoring is necessary.

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Section: Discussionsupporting

confidence: 67%

A three-years assessment of Ixodes ricinus-borne pathogens in a French peri-urban forest

et al. 2019

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“…A previous study on the microbiome of I. ricinus also observes an unstable microbial composition (Aivelo et al, 2019a(Aivelo et al, , 2019b. However, contrary to our findings, Aivelo et al found M. mitochondrii in all sequenced ticks (n = 82).…”

Section: Limited Microbiome Of Ixodes Ricinuscontrasting

confidence: 99%

“…and Francisella spp., and Lariskella spp. (Aivelo et al, 2019a(Aivelo et al, , 2019bSpitalska et al, 2018;Wojcik-Fatla et al, 2015). However, it is unknown what their functional role is and whether they are genuinely associated with this tick species.…”

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confidence: 99%

Questing microbioticks : Interactions of microbes, ticks, vertebrates, and the environment

Krawczyk

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The influence of external (a)biotic factors on the maintenance of symbionts in tick populations depends much on symbionts' mode of transmission. (this thesis) <. Ticks choose their symbionts rather than symbionts choose their ticks. (this thesis) =. An infection that is a burden for an individual can be beneficial for a population. @. The !AS rRNA amplicon sequencing technique is a powerful tool for forming hypotheses. H. We are selective about who or what should be a member of the (bio)diversity we want to preserve. A. Nowadays, it is difficult to distinguish what we really think from what we have read online.

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“…Which spatial scale is the best investigation of the tick microbiome? Field studies have shown the strong influence of biogeography at large scales (states or regions) on the tick microbiome structure and composition on different tick species, I. scapularis [66], I. ricinus [109][110][111] and A.…”

Section: Is the Spatial Scale Necessary To Efficiently Study The Tickmentioning

confidence: 99%

The scale affects our view on the identification and distribution of microbial communities in ticks

et al. 2020

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Ticks transmit the highest variety of pathogens impacting human and animal health worldwide. It is now well established that ticks also harbour a microbial complex of coexisting symbionts, commensals and pathogens. With the development of high throughput sequencing technologies, studies dealing with such diverse bacterial composition in tick considerably increased in the past years and revealed an unexpected microbial diversity. These data on diversity and composition of the tick microbes are increasingly available, giving crucial details on microbial communities in ticks and improving our knowledge on the tick microbial community. However, consensus is currently lacking as to which scales (tick organs, individual specimens or species, communities of ticks, populations adapted to particular environmental conditions, spatial and temporal scales) best facilitate characterizing microbial community composition of ticks and understanding the diverse relationships among tick-borne bacteria. Temporal or spatial scales have a clear influence on how we conduct ecological studies, interpret results, and understand interactions between organisms that build the microbiome. We consider that patterns apparent at one scale can collapse into noise when viewed from other scales, indicating that processes shaping tick microbiome have a continuum of variability that has not yet been captured. Based on available reports, this review demonstrates how much the concept of scale is crucial to be considered in tick microbial community studies to improve our knowledge on tick microbe ecology and pathogen/ microbiota interactions.

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Human pathogen co-occurrence in Ixodes ricinus ticks: effects of landscape topography, climatic factors and microbiota interactions

Abstract: 5The factors shaping microbial communities within organisms are still poorly understood. Besides

Cited by 9 publications

References 99 publications

A three-years assessment of Ixodes ricinus-borne pathogens in a French peri-urban forest

A three-years assessment of Ixodes ricinus-borne pathogens in a French peri-urban forest

Questing microbioticks : Interactions of microbes, ticks, vertebrates, and the environment

The scale affects our view on the identification and distribution of microbial communities in ticks

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