Human periodontal ligament stem cells (hPDLSCs) spontaneously differentiated into myofibroblast to repairer diabetic wounds

Abstract

Diabetic ulcer healing is affected by persistent hyperglycemia, and the current treatment methods are extremely limited. We aim to take the advantages of stem cell therapy and try to use periodontal ligament stem cells that have not yet become therapeutic cells to repair diabetic wounds, so as to provide theoretical basis for its application in clinical practice. Methods: The effect of AGEs on the myofibroblast differentiation ability of hPDLSCs was investigated by RT-qPCR and WBto detect the gene and protein expressions of α-SMA and COLIA1. HE staining and Masson staining were used to observe the effect of hPDLSCs on wound healing in STZ-induced diabetic mice, and immunohistochemical staining was used to detect the expression of α-SMA and CD31. Immunofluorescence (IF) was used to detect the expression of CD206 and iNOS in the tissue of wound area.hPDLSCs can spontaneously differentiate into myoblasts during culture. In α-MEM medium, at 25μg/mL AGEs, the expression of COLIA1 in the seventh passage (P7) cells was significantly decreased, and α-SMA was increased (P < 0.05). hPDLSCs hydrogel injection was used to heal the wounds of C57 mice, and it was found that the healing rate of hPDLSCs group was 65.247% on the 7th day of healing while that of the model group was 48.938%. The healing rate of hPDLSCs treatment group was much higher than that of the model group. Conclusion: hPDLSCs can repair the skin wounds of diabetic mice by spontaneously differentiating into myofibroblasts and resisting the damage of AGEs, which is expected to be used in clinical diagnosis and treatment.