Human peripheral blood CD4+ and CD8+ T cells express Th1-like cytokine mRNA and proteins following in vitro stimulation with heat-inactivated Brucella abortus

Zaitseva, Marina; Golding, Hana; Betts, Michael R.; Yamauchi, Akira; Bloom, Eda T.; L, Butler; Stevan, L; Golding, Basil

doi:10.1128/iai.63.7.2720-2728.1995

Cited by 63 publications

(31 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Accordingly, a CD14 + /CD80 + percentage increase might compensate for an ineffectual CD4 + T cell response in patients with chronic relapsing brucellosis through the induction of CD8 + T lymphocytes. Activated CD8 + T cells participate in protection against Brucella in humans, through cytotoxicity or/and by the production of IFN‐γ[9–11].…”

Section: Discussionmentioning

confidence: 99%

“…Brucella can reside within macrophages of the host and invades the normal mechanisms of bacterial killing, causing chronic disease [7,9]. Adequate T helper1 (T h1) immune response is fundamental for the clearance of Brucella infection and Brucella antigens induce the production of Th1 cytokines in humans [9–11]. Diminished production of Th1 cytokines [interferon (IFN)‐γ and interleukin (IL)‐2] has been associated with T cell unresponsiveness to Brucella antigens and disease chronicity [12,13].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

CD80/CD28 co-stimulation in human brucellosis

Skendros

Boura

Kamaria³

et al. 2006

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SummaryDespite treatment, 10-30% of brucellosis patients develop chronic disease, characterized by atypical clinical picture and/or relapses. A defective T helper 1 (Th1) response and a long percentage of CD4 + /CD25 + cells have been described in chronic brucellosis patients. CD80/CD28 co-stimulation is critical for an efficient Th1 response and has not been studied previously in human brucellosis. In order to investigate the role of CD80/CD28 co-stimulation, 13 acute brucellosis patients (AB), 22 chronic brucellosis patients (CB, 12/22 relapsing type-CB1 and 10/22 atypical type-CB2), 11 'cured' subjects and 15 healthy volunteers (controls) were studied. The percentage of CD4 + /CD28 + T lymphocytes and CD14 + /CD80 + monocytes were analysed by flow cytometry both ex vivo and after phytohaemagglutinin (PHA)-stimulation with or without heat-killed Brucella abortus (HkBA). Ex vivo analysis showed no differences between all groups studied. PHA stimulation up-regulated the percentage of CD80 + monocytes in AB compared to 'cured' subjects and controls (P < 0·001), although the proportion of CD4 + / CD28 + cells did not alter. A higher percentage of CD80 + monocytes was observed in the CB1 subgroup, compared to AB, 'cured' subjects and controls (P = 0·042, < 0·001 and < 0·001, respectively). CB2 was characterized by a lower percentage of CD80 + monocytes in comparison to CB1 (P = 0·020). HkBA in PHA cultures down-regulated the percentage of CD80+ monocytes compared to PHA alone in all groups, especially in AB and CB patients (P < 0·001 and P = 0·007, respectively). In conclusion, the diminished percentage of CD4 + /CD25 + T cells in CB is not associated with inadequate CD80/ CD28 co-stimulation. We speculate that differential frequency of CD80 + monocytes after PHA stimulation could serve as a qualitative parameter of disease status, related to the different clinical forms of chronic brucellosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

CD80/CD28 co-stimulation in human brucellosis

Skendros

Boura

Kamaria³

et al. 2006

Clinical and Experimental Immunology

View full text Add to dashboard Cite

show abstract

“…This would be in agreement with studies performed by others. Zaitseva et al showed production of IL-10 and IFN-)I by human T cells stimulated with B. abortus but only a low level of IL-4 mRNA and no detectable IL-4 protein [19].…”

Section: Discussionmentioning

confidence: 99%

Comparison of T cell cytokines in resistant and susceptible mice infected with virulent Brucella abortus strain 2308

Fernandes

1996

FEMS Immunology and Medical Microbiology

View full text Add to dashboard Cite

C57BL/10 and BALB/c mice differ in their abilities to clear infections with the intracellular bacterium Brucella abortus strain 2308. We have previously reported that in vivo of IL-10 in the susceptible BALB/c mice results in significantly fewer bacteria in their spleens 1 week after infection with 5 x 10(3) colony forming units (CFU) of 2308. Here we extend those studies and report a similar effect when IL-4 is neutralized. In contrast, in the more resistant C57BL/10 mice infected with 5 x 10(3) CFU, neither neutralization of IL-4 significantly decreased the level of infection nor did it in either BALB/c or C57BL/10 mice infected with a 1000-fold higher dose of strain 2308. While splenocytes from the later mentioned groups of mice produced IL-10 in response to stimulation with brucella antigen, they also produced higher levels of interferon (IFN)-gamma than those from BALB/c mice with the low challenge dose of 5 x 10(3) CFU. Results of in vivo neutralization of IFN-gamma by monoclonal antibodies (MAb) reported here and elsewhere indicated that IFN-gamma is important for control; thus, we postulate that the higher levels of IFN-gamma override the detrimental effects of Th2 cytokines. In vitro studies also showed that macrophages from the more resistant C57BL/10 mice were less susceptible to the ability of IL-10 to decrease anti-brucella activities than were BALB/c macrophages. CD4+ T cells were principally responsible for the production of IL-10 in BALB/c but not C57BL/10 splenocyte populations. C57BL/10 splenocytes produced more IFN-gamma than those from BALB/c mice in response to stimulation with brucella antigens. These differences between BALB/c and C57BL/10 mice may contribute to the superior capacity of C57BL/10 mice to control infections with B. abortus strain 2308.

show abstract

“…In turn, the functional capacity of T cells following activation is dependent on numerous factors, including the dose of antigen used during stimulation, the type of costimulatory signals received from accessory cells, and the differentiated state of the T cells before activation. Thus, multiple examples have been described where the extent of antigen-induced lymphocyte proliferation has been shown to be completely independent of other immune activities, such as production of IL-2 and other cytokines or the ability to provide help for immunoglobulin synthesis by B cells [33][34][35]. Interestingly, we found that the individuals whose T cells proliferated well in response to Vespula venom also tended to have higher levels of venom-specific IgE, suggesting that the activities associated with the induction of T cell proliferation may also promote IgE synthesis.…”

Section: Discussionmentioning

confidence: 99%

Characterization of proliferative responses and cytokine mRNA profiles induced by Vespula venom in patients with severe reactions to wasp stings

Bonay

Echchakir

Lecossier

et al. 1997

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYThe reasons why severe allergic reactions to bee and wasp stings develop in only a small portion of exposed individuals are incompletely understood, but differences in T cell responses to venom antigens comparing allergic and non-allergic individuals are likely to be important. To identify such differences, venom-induced proliferative responses and cytokine mRNA production by blood mononuclear cells from Vespula venom-allergic patients and non-allergic individuals were compared. Mononuclear cells from most venom-allergic patients proliferated in response to alkylated Vespula venom (7275 Ϯ 8387 ct/min, n ¼ 19), and the extent of proliferation was greater for patients with a history of multiple prior stings and those with high levels of venom-specific IgE. Although mononuclear cells from non-allergic subjects showed little or no proliferation in response to venom (926 Ϯ 711 ct/min, n ¼ 8), production of mRNAs coding for IL-2, IL-4, IL-5, IL-10 and interferon-gamma (IFN-g) in response to Vespula venom by cells from non-allergic subjects was detected by reverse transcriptase-polymerase chain reaction (RT-PCR), indicating that these individuals had been previously sensitized to venom antigens. In contrast to the Th0 cytokine mRNA profile observed for non-allergic individuals, venom-allergic patients released a more restricted profile of cytokines following stimulation with venom. Only IFN-g mRNA expression was detected in all individuals evaluated, whereas IL-2 mRNA was not detected during the first 48 h of stimulation, and T cells from only one of three venom-allergic individuals produced detectable IL-4 or IL-5 mRNA. The difference in cytokine profiles observed comparing venom-allergic patients and non-allergic controls could not be attributed to intrinsic differences in T cells from these individuals, because polyclonal stimulation with phorbol myristate acetate (PMA) + ionophore induced similar cytokine mRNA profiles in the two groups. These studies demonstrate clear differences in the T cell responses of venom-allergic subjects, that may contribute to the development of severe allergic reactions in these individuals.

show abstract

Human peripheral blood CD4+ and CD8+ T cells express Th1-like cytokine mRNA and proteins following in vitro stimulation with heat-inactivated Brucella abortus

Cited by 63 publications

References 37 publications

CD80/CD28 co-stimulation in human brucellosis

CD80/CD28 co-stimulation in human brucellosis

Comparison of T cell cytokines in resistant and susceptible mice infected with virulent Brucella abortus strain 2308

Characterization of proliferative responses and cytokine mRNA profiles induced by Vespula venom in patients with severe reactions to wasp stings

Contact Info

Product

Resources

About