Human Placenta‐Derived Cells Have Mesenchymal Stem/Progenitor Cell Potential

Abstract

IntroductionMultipotential mesenchymal stem/progenitor cells (MSCs) can be induced to differentiate into bone, adipose, cartilage, muscle, and endothelium if these cells are cultured under specific permissive conditions [1,2]. In rodents, a specific type of MSC (termed multipotent adult progenitor cell) can be isolated from bone marrow (BM) and contributes to most somatic cell types when injected into early blastocysts at the single-cell level [3] , kidney, lung, and liver). These cells are also present in the fetal environment (e.g., blood, liver, BM, and kidney). However, MSCs are a rare population in these tissues. Here we tried to identify cells with MSC-like potency in human placenta. We isolated adherent cells from trypsin-digested term placentas and established two clones by limiting dilution. We examined these cells for morphology, surface markers, gene expression patterns, and differentiation potential and found that they expressed several stem cell markers, hematopoietic/ endothelial cell-related genes, and organ-specific genes, as determined by reverse transcription-polymerase chain reaction and fluorescence-activated cell sorter analysis. They also showed osteogenic and adipogenic differentiation potentials under appropriate conditions. We suggest that placenta-derived cells have multilineage differentiation potential similar to MSCs in terms of morphology, cell-surface antigen expression, and gene expression patterns. The placenta may prove to be a useful source of MSCs.

Inuteroarsenic exposure and epigenome-wide associations in placenta, umbilical artery, and human umbilical vein endothelial cells

“…47 Furthermore, it has also been observed that the human placenta contains both haematopoietic stem cells and mesenchymal stem cells. 48 All tissues derived from the fetus are an extension of the mesoderm that differentiates during embryonic development to form the umbilical cord and placenta. Therefore, we further hypothesize that exposure to arsenic during fetal development could affect cellular differentiation and lineage commitment for placenta and artery but not HUVEC as this is a cellular homogenous tissue.…”

Cardiac Repair Using Stem Cells Conditioned Media Based Therapy in a Rodent Model of Myocardial Infarction and Heart Failure

“…It was first discovered back in the mid-sixties in the bone marrow 42 then later on it was found in other different tissues such as adipose tissue, placenta and muscles. 43 1. One very important aspect of HiPSCs is that, they can be aimed to differentiate into a specified lineage which unlocked many opportunities for the research in regenerative medicine, therapeutic for disease modeling and drug discovery.…”

Age-Related Decreases of Serum-Response Factor Levels in Human Mesenchymal Stem Cells Are Involved in Skeletal Muscle Differentiation and Engraftment Capacity

“…S1; Supplementary Data are available online at www.liebertpub.com/scd) [9,11,21,22,29,30]. The ability of hDE-MSCs to differentiate into various myogenic lineages, however, has not been investigated.…”

“…hDE-MSCs, as a type of MSCs, are capable of multilineage differentiation [9,11,21,22,29,30], and we sought to ascertain the specificity of MM-induced SkM differentiation in these MSCs by assaying for changes in the levels of Runx2 and PPAR-c, the master lineage transcription factors for osteogenesis and adipogenesis, respectively [40,41]. We found that in all types of hDEMSCs undergoing SkM differentiation, the expression levels of Runx2 and PPAR-c are decreased compared with undifferentiated hDE-MSCs (Supplementary Fig.…”

“…Increasing number of reports show that fetal tissue and fetal extraembryonic tissues such as umbilical cord and placenta are good sources for MSCs [6,[8][9][10][11]. These developmentally early stage mesenchymal stem cells (DE-MSCs) can be isolated without performing additional invasive procedures, and these are more proliferative than adult BMMSCs [12,13].…”