Human placental extracts suppress mast cell activation and induce mast cell apoptosis

Abstract

Background Human placental extracts (HPE) have been documented to facilitate the healing of certain disorders including allergy. However, the effects of HPE on the functionality of mast cells, a critical cell type in allergic diseases, have not been reported. Methods To investigate the effects of HPE on the regulation of allergy with respect to the biological functions of mast cells, the mast cell line C57 cells were treated with HPE followed by the assessment of cell proliferation, apoptosis, activation, chemotaxis and phagocytosis. Mouse peritoneal mast cells were also investigated for their responses to induction of apoptosis by HPE in vivo. Furthermore, the effect of HPE on mast cell degranulation was confirmed using the passive cutaneous anaphylaxis (PCA) assay, an acute allergy model. Results HPE was capable of suppressing mast cell proliferation and inducing mast cell apoptosis. Mast cell degranulation in response to compound 48/80- or anti-DNP IgE and DNP-mediated activation was suppressed. In addition, treatment with HPE compromised the production of cytokines by mast cells and cell chemotaxis. These observations were consistent with the dampened passive cutaneous anaphylaxis (PCA) assay following treatment with HPE. Conclusion This study revealed a suppressive effect of HPE on overall mast cell activities, suggesting a potential regulatory role of HPE on the alleviation of allergic diseases through mast cells.

Histopathological Evaluation of Human Placental Extract as a Direct Pulp-Capping Material in Dogs' Teeth

“…34 The quest to find novel materials whose properties can be harnessed for dental regeneration due to lack of an optimal pulp-capping material is the primary driving force behind the investigation of further pulp capping strategies. Based on the biologically based wound-healing strategies of HPE, 35,36 it was evaluated in the current study as it may be of value in the future of vital pulp therapy targeting pulp regeneration processes. Dogs were chosen as the study's animal model in the current study due to similarity of reparative dentin formation to that in humans, however, occurs at a faster rate.…”