Human placental study of genetics/genomic, environmental contaminant and morphology assessments from 12 U.S. Counties – Methods and results from the U.S. National Children’s Study (NCS)

Miller, Richard K.; Stodgell, Christopher J.; Katzman, Philip J.; Friedman, Alan E.; Friedman, Michelle; Ruffolo, Louis; Penmetsa, Amulya; Salamone, Linda; Salafia, Carolyn M.; Jia, Chen; Schadt, Eric E.; Hao, Ke; Littman, Lisa; Landrigan, Philip J.; Walker, Cheryl K.; Darrah, Thomas H.; Liu, An; Dassayake, Priyanthi S.; Szabo, S.; Aagaard, Kjersti M.; Hobbs, Charlotte A.; Clark, Edward B.; Varner, Michael W.; Dole, Nancy; Culhane, Jennifer F.; Swanson, James M.; Thiex, Natalie W.; Murray, Jeffrey; Busch, Tamara; Misra, Dawn P.; Durkin, Maureen S.; Moyle, John; Kasten, Carol H.; O’Brien, Barbara

doi:10.1016/j.placenta.2014.06.007

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a pilot study we determined the temporal variability in placental miRNA expression level based on repeat biopsies sampled at varying time-points. 62,63 Based on our findings from this prior study, we limited our analysis to placenta samples that were collected within 6 h of delivery to ensure miRNA stability (n D 189). Basic clinical information, including infant gender, gestational age, delivery method, and absence of any pathological abnormalities, was available for 141 subjects.…”

Section: Study Populationmentioning

confidence: 99%

Exploring the associations between microRNA expression profiles and environmental pollutants in human placenta from the National Children's Study (NCS)

Kappil

Liu

et al. 2015

Epigenetics

Self Cite

View full text Add to dashboard Cite

The placenta is the principal regulator of the in utero environment, and disruptions to this environment can result in adverse offspring health outcomes. To better characterize the impact of in utero perturbations, we assessed the influence of known environmental pollutants on the expression of microRNA (miRNA) in placental samples collected from the National Children's Study (NCS) Vanguard birth cohort. This study analyzed the expression of 654 miRNAs in 110 term placentas. Environmental pollutants measured in these placentas included dichlorodiphenyldichloroethylene (DDE), bisphenol A (BPA), polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs), arsenic (As), mercury (Hg), lead (Pb), and cadmium (Cd). A moderated t-test was used to identify a panel of differentially expressed miRNAs, which were further analyzed using generalized linear models. We observed 112 miRNAs consistently expressed in >70% of the samples. Consistent with the literature, miRNAs located within the imprinted placenta-specific C19MC cluster, specifically mir-517a, mir-517c, mir-522, and mir-23a, are among the top expressed miRNA in our study. We observed a positive association between PBDE 209 and miR-188-5p and an inverse association between PBDE 99 and let-7c. Both PCBs and Cd were positively associated with miR-1537 expression level. In addition, multiple let-7 family members were downregulated with increasing levels of Hg and Pb. We did not observe DDE or BPA levels to be associated with placental miRNA expression. This is the first birth cohort study linking environmental pollutants and placental expression of miRNAs. Our results suggest that placental miRNA profiles may signal in utero exposures to environmental chemicals.

show abstract

Section: Study Populationmentioning

confidence: 99%