Human platelet lysate enhances proliferation but not chondrogenic differentiation of pediatric mesenchymal progenitors

Gardner, Oliver F. W.; Agabalyan, Natacha A.; Weil, Ben H.; Ali, Mohammed H I; Lowdell, Mark W.; Bulstrode, Neil; Ferretti, Patrizia

doi:10.1016/j.jcyt.2022.11.007

Cited by 4 publications

(2 citation statements)

References 57 publications

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“…As far as we are aware, this has not been explored in a 3D collagen model, however, studies in other types of gels show differing effects of PLT on the differentiation [ 35 , 53 ]. Interestingly, a recently published article showed that PLT improved the proliferation of pediatric human adipose-derived stem cell (ADSCs) and auricular cartilage stem/progenitor cells in both 2D and 3D (pellet) culturing systems, while impairing chondrogenic differentiation [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Platelet lysate for expansion or osteogenic differentiation of bone marrow mesenchymal stem cells for 3D tissue constructs

Anerillas

Wiberg

Kingham

et al. 2023

Regenerative Therapy

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Section: Discussionmentioning

confidence: 99%

Platelet lysate for expansion or osteogenic differentiation of bone marrow mesenchymal stem cells for 3D tissue constructs

Anerillas

Wiberg

Kingham

et al. 2023

Regenerative Therapy

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“…Recent studies found that HPLs can stimulate the propagation of pediatric auricular cartilage stem/progenitor cells, enhanced the preservation of a spindle-like morphology, with good preservation of surface marker expression, and supported expressions of collagen type II and aggrecan. However, defects in matrix deposition were observed [ 187 ]. More systematic studies are deemed necessary to understand the functional effects of HPLs and determine the type and dose of HPLs optimal for chondrocyte expansion.…”

Section: Use Of Allogeneic Hpl For Cell Propagationmentioning

confidence: 99%

Expanding applications of allogeneic platelets, platelet lysates, and platelet extracellular vesicles in cell therapy, regenerative medicine, and targeted drug delivery

Burnouf,

Chou,

Lundy

et al. 2023

J Biomed Sci

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Platelets are small anucleated blood cells primarily known for their vital hemostatic role. Allogeneic platelet concentrates (PCs) collected from healthy donors are an essential cellular product transfused by hospitals to control or prevent bleeding in patients affected by thrombocytopenia or platelet dysfunctions. Platelets fulfill additional essential functions in innate and adaptive immunity and inflammation, as well as in wound-healing and tissue-repair mechanisms. Platelets contain mitochondria, lysosomes, dense granules, and alpha-granules, which collectively are a remarkable reservoir of multiple trophic factors, enzymes, and signaling molecules. In addition, platelets are prone to release in the blood circulation a unique set of extracellular vesicles (p-EVs), which carry a rich biomolecular cargo influential in cell–cell communications. The exceptional functional roles played by platelets and p-EVs explain the recent interest in exploring the use of allogeneic PCs as source material to develop new biotherapies that could address needs in cell therapy, regenerative medicine, and targeted drug delivery. Pooled human platelet lysates (HPLs) can be produced from allogeneic PCs that have reached their expiration date and are no longer suitable for transfusion but remain valuable source materials for other applications. These HPLs can substitute for fetal bovine serum as a clinical grade xeno-free supplement of growth media used in the in vitro expansion of human cells for transplantation purposes. The use of expired allogeneic platelet concentrates has opened the way for small-pool or large-pool allogeneic HPLs and HPL-derived p-EVs as biotherapy for ocular surface disorders, wound care and, potentially, neurodegenerative diseases, osteoarthritis, and others. Additionally, allogeneic platelets are now seen as a readily available source of cells and EVs that can be exploited for targeted drug delivery vehicles. This article aims to offer an in-depth update on emerging translational applications of allogeneic platelet biotherapies while also highlighting their advantages and limitations as a clinical modality in regenerative medicine and cell therapies.

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The influence of xeno-free culture conditions on the angiogenic and adipogenic differentiation properties of adipose tissue-derived stem cells

Lauvrud,

Giraudo,

Wiberg

et al. 2024

Regenerative Therapy

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Human platelet lysate enhances proliferation but not chondrogenic differentiation of pediatric mesenchymal progenitors

Cited by 4 publications

References 57 publications

Platelet lysate for expansion or osteogenic differentiation of bone marrow mesenchymal stem cells for 3D tissue constructs

Platelet lysate for expansion or osteogenic differentiation of bone marrow mesenchymal stem cells for 3D tissue constructs

Expanding applications of allogeneic platelets, platelet lysates, and platelet extracellular vesicles in cell therapy, regenerative medicine, and targeted drug delivery

The influence of xeno-free culture conditions on the angiogenic and adipogenic differentiation properties of adipose tissue-derived stem cells

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