Human platelet‐specific antigens frequencies in the Argentinean population

Abstract

SUMMARY. The frequencies of several human platelet antigens (HPAs) vary between different populations and are a major determinant for the prevalence of HPA alloimmunization and its clinical associated entities. The aim of this study was to characterize the allele frequencies of seven HPA systems in two different ethnic groups from the Argentinean city of Rosario, the major population and a minority Amerindian group recently arrived from the north of the country, the Tobas. A total of 192 healthy unrelated individuals from blood donors and hospital staff from the Hospital Italiano Garibaldi and 27 unrelated Toba Amerindians were genotyped for HPA-1, -2, -3, -4, -5, -6 and -15 systems by polymerase chain reaction with sequence specific primers (PCR-SSP). The present data showed that the distribution of the HPA alleles among Argentineans from Rosario is quite similar to that reported among Europeans. The frequencies seen in Tobas, although limited by the small number of aboriginal samples studied, are similar to those reported for other Amerindians populations. Statistically significant differences were found for the genotype distribution of HPA-1, -3, -5 and -15 between both groups, indicating important differences in the potential risk of HPA alloimmunization associated to transfusion and pregnancy. The study of these polymorphisms represents the first step in the elucidation of pathological conditions that are underdiagnosed in our population. It allowed us to establish a panel of characterized blood donors necessary for the serological work out and as a source for compatible platelets transfusion.

Human platelet antigen genotyping of platelet donors in southern Brazil

“…Comparison of the HPA frequencies observed in the present study with those reported in Asian (Seo et al ., ; Nie et al ., ; Tan et al ., ; Asmarinah et al ., ), Oceanic (Edinur et al ., ), Amazonian Indians (Castro et al ., ) and Toba Amerindian (De La Vega Elena et al ., ) populations yielded significant differences. These differences may be explained by the negligible participation of these demographic and ethnic groups in the population of Rio Grande do Sul.…”

“…No significant differences in allele frequencies were observed between our Caucasian group and the populations of Argentina (De La Vega Elena et al ., ; HPA‐1 to 5 and 15), Italy (EBI, ; HPA‐1 to 5), Germany (EBI, ; HPA‐1 to 3 and 5), Croatia (Pavic et al ., ; HPA‐1 to 3 and 5), the United Kingdom (Jones et al ., ; HPA‐1 to 5), Slovenia (Rozman et al ., ; HPA‐1 to 5), France (Merieux et al ., ; HPA‐1 to 5), Denmark (Steffensen et al ., ; HPA‐1 to 5), Austria (Holensteiner et al ., ; HPA‐1 to 3 and 5), Poland (Drzewek et al ., ; HPA‐1 to 5) or Spain (Muniz‐Diaz et al ., ; HPA‐2 to 5 and 15). For HPA‐1, we found a significant difference between our sample and the Spanish population (HPA‐1a = 0.867 and 1b = 0.133 vs. HPA‐1a = 0.810 and 1b = 0.190, respectively, P < 0.05).…”

“…To assess the ability of HPA alleles to describe intercontinental genetic divergence, we conducted an amova , which quantifies separation between populations. Towards this end, we divided the assessed populations into two groups: one comprising the populations of Asia and Oceania, as well as native ethnic groups – Toba Amerindians (De La Vega Elena et al ., ), Parakanã Indians (Castro et al ., ), Native Brazilians, Maori people (Edinur et al ., ), Korea (Pavic et al ., ), Malaysia (Tan et al ., ), Indonesia (Asmarinah et al ., ), China (Nie et al ., ) and Polynesia (Edinur et al ., ) – and one comprising the populations of European and African origin: Poland (Drzewek et al ., ), Denmark (Steffensen et al ., ), Italy (EBI, ), Spain (Muniz‐Diaz et al ., ), the United Kingdom (Jones et al ., ), Slovenia (Rozman et al ., ), Argentina (De La Vega Elena et al ., ), the Congo (Halle et al ., ) and Egypt (Salem et al ., ). Argentina was included in this group because the makeup of its population is strongly European.…”

A study of human neutrophil antigen genotype frequencies in Hong Kong

“…It can be anticipated that the Chinese in Hong Kong, Guangzhou or other regions might be similarly at low risk of TRALI, ANN and other anti‐neutrophil antibody‐related diseases. As suggested by De la Vega et al ., () the estimated risk might be relatively low if there was less difference in HNA genotype distribution (Shen et al, ). Our local data support the hypothesis that there is no additional risk of anti‐neutrophil antibody‐related diseases arising from transfusion among Chinese populations.…”

Human platelet antigens frequencies in Maori and Polynesian populations

“…Probabilities of transfusion and pregnancy alloimmunization were calculated according to De La Vega Elena et al . ().…”