Human platelets can discriminate between various bacterial LPS isoforms via TLR4 signaling and differential cytokine secretion

Berthet, J; Damien, Pauline; Hamzeh‐Cognasse, Hind; Arthaud, Charles‐Antoine; Eyraud, Marielle; Zéni, Fabrice; Pozzetto, Bruno; McNicol, Archibald; Garraud, Olivier; Cognasse, Fabrice

doi:10.1016/j.clim.2012.09.004

Cited by 124 publications

(139 citation statements)

References 66 publications

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“…E. coli LPS did result in increased PS positivity. Previous studies have also shown that platelets can react differently to various forms of LPS, and suggested that this might be due to differences in TLR4 and CD14 binding (29) or structure of the O-antigen region of LPS and activation of the lectin pathway of the complement system (30). Platelets do not express CD14, but can absorb large amounts from plasma (31), which might also play a role in our results -as stimulations were performed in platelet rich plasma.…”

Section: Discussionmentioning

confidence: 99%

Platelet Toll-like receptor expression and activation induced by lipopolysaccharide and sepsis

et al. 2018

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Platelets and Toll-like receptor (TLR) signalling play a role in the immune response during sepsis. Although preclinical knowledge about the role of platelet TLR signalling is increasing, data during human sepsis are less abundant. Moreover, controversy remains about the effect of the TLR4 agonist lipopolysaccharide (LPS) on platelet activation. We therefore assessed platelet TLR expression during human and murine sepsis. Moreover, we investigated the effect of TLR4 signalling on platelet activation and TLR expression. Platelets from healthy controls stimulated with LPS did not show classical platelet activation (P-selectin, CD63 and phosphatidylserine expression), potentiation of subthreshold agonist stimulation nor platelet-leukocyte complex formation. LPS stimulation however did increase maximal mitochondrial respiration in a TLR4-dependent manner. Platelet stimulation with LPS did not alter TLR expression. Platelet stimulation with thrombin receptor activating peptide increased TLR5 and TLR9, but not TLR2 or TLR4 expression. Platelets from patients with sepsis and mice with experimental sepsis showed platelet activation, but unaltered TLR expression. These results indicate that sepsis-induced platelet activation is not associated with altered platelet TLR expression and, although platelets are responsive to LPS, stimulation of platelet TLR4 does not result in classical platelet activation.

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Section: Discussionmentioning

confidence: 99%

Platelet Toll-like receptor expression and activation induced by lipopolysaccharide and sepsis

et al. 2018

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“…CD14 is a glycoprotein located on the cell surface and is mainly produced by monocytes, macrophages, and neutrophils (Parajuli et al, 2012). CD14 has been shown to be involved in primary immune and inflammatory responses (Apetoh et al, 2007;Berthet et al, 2012;Guo et al, 2013). Studies have demonstrated that genetic variations in CD14 may change the structure of the CD14 protein and influence CD14-lipopolysaccharide (LPS) interactions (Baldini et al, 1999;Karhukorpi et al, 2002).…”

Section: Introductionsmentioning

confidence: 99%

Association of CD14 C159T polymorphism with atopic asthma susceptibility in children from Southeastern China: a case-control study

Zhang

et al. 2015

Genet. Mol. Res.

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ABSTRACT. CD14 is involved in primary immune and inflammatory responses. The -159 C/T variation in the CD14 gene plays an important role in regulating CD14 expression and has been associated with the susceptibility to various diseases, including allergies. In this study, we examined the association between the C-159T polymorphism and atopic asthma susceptibility in children from Southeastern China. The study population included 746 unrelated children of Chinese Han nationality (362 patients with atopic asthma and 384 healthy controls). CD14 gene polymorphisms were identified by direct sequencing of polymerase chain reaction products. Total immunoglobulin E (IgE) levels in human serum samples were determined using an enzymelinked immunosorbent assay. Individuals carrying the TT genotypes for rs2569190 were significantly associated with an increased risk of atopic asthma compared with those carrying the wild-type homozygous CC genotypes [adjusted odds ratio (OR) by gender and age, from 1.075- 2.398, P = 0.025]. Total serum IgE levels in TT genotype carriers were significantly higher than those in CC genotype carriers in atopic asthma patients (286.3 ± 161.5 IU/mL vs 248.3 ± 147.8 IU/mL). Our data suggest that the CD14 TT genotype may be a genetic susceptibility marker for atopic asthma in Chinese Han children.

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“…Les plaquettes, qui expriment des Toll-like receptors ou TLR [33] peuvent aussi lier des dérivés microbiens bactériens, viraux ou parasitaires, qui activent ces cellules (pourvues d'un inflammasome), et qui déclenchent la sécrétion différenciée de cytokines pro-inflammatoires, selon des programmes ressemblant aux programmes de différenciation des cellules nucléées [20,34]. Le sepsis (grande manifestation inflammatoire) est un bon exemple de ces interactions complexes entre les plaquettes et les agents infectieux [35,36].…”

Section: Agents Infectieux Ou Leurs Produits De Dégradationunclassified

Transfusion sanguine et inflammation

Garraud

Cognasse

Hamzeh‐Cognasse

et al. 2013

Transfusion Clinique et Biologique

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Human platelets can discriminate between various bacterial LPS isoforms via TLR4 signaling and differential cytokine secretion

Cited by 124 publications

References 66 publications

Platelet Toll-like receptor expression and activation induced by lipopolysaccharide and sepsis

Platelet Toll-like receptor expression and activation induced by lipopolysaccharide and sepsis

Association of CD14 C159T polymorphism with atopic asthma susceptibility in children from Southeastern China: a case-control study

Transfusion sanguine et inflammation

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