Human Platelets Loaded with Trehalose Survive Freeze-Drying

Wolkers, Willem F.; Walker, Naomi J.; Tablin, Fern; Crowe, John H.

doi:10.1006/cryo.2001.2306

Cited by 303 publications

(233 citation statements)

References 18 publications

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“…For example, an increase in trehalose content in brain or muscle by exogenously expressing TRET1 is expected to alleviate Huntington's disease or oculopharyngeal muscular dystrophy, respectively. Likewise, cells and organs loaded with a high concentration of trehalose may be cryopreserved more safely and might even be lyophilized for preservation such as the case for human platelets (13,14). In addition to these applications, there are benefits for glycotechnology because strict substrate specificity of TRET1 provides major advantages in the screening of newly synthesized trehalose analogs.…”

Section: Discussionmentioning

confidence: 99%

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Trehalose transporter 1, a facilitated and high-capacity trehalose transporter, allows exogenous trehalose uptake into cells

Kikawada

Saito

Kanamori

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

227

211

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Trehalose is potentially a useful cryo-or anhydroprotectant molecule for cells and biomolecules such as proteins and nucleotides. A major obstacle to application is that cellular membranes are impermeable to trehalose. In this study, we isolated and characterized the functions of a facilitated trehalose transporter [trehalose transporter 1 (TRET1)] from an anhydrobiotic insect, Polypedilum vanderplanki. Tret1 cDNA encodes a 504-aa protein with 12 predicted transmembrane structures. Tret1 expression was induced by either desiccation or salinity stress. Expression was predominant in the fat body and occurred concomitantly with the accumulation of trehalose, indicating that TRET1 is involved in transporting trehalose synthesized in the fat body into the hemolymph. Functional expression of TRET1 in Xenopus oocytes showed that transport activity was stereochemically specific for trehalose and independent of extracellular pH (between 4.0 and 9.0) and electrochemical membrane potential. These results indicate that TRET1 is a trehalose-specific facilitated transporter and that the direction of transport is reversible depending on the concentration gradient of trehalose. The extraordinarily high values for apparent K m (>100 mM) and Vmax (>500 pmol/min per oocyte) for trehalose both indicate that TRET1 is a high-capacity transporter of trehalose. Furthermore, TRET1 was found to function in mammalian cells, suggesting that it confers trehalose permeability on cells, including those of vertebrates as well as insects. These characteristic features imply that TRET1 in combination with trehalose has high potential for basic and practical applications in vivo.anhydrobiosis ͉ insect ͉ trehalose transport ͉ Polypedilum vanderplanki dessication-inducible gene

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Section: Discussionmentioning

confidence: 99%

“…Human platelets have been successfully freeze-dried after incorporation of trehalose by pinocytosis (13,14), showing that trehalose plays an important role as a cryo-and anhydroprotectant. Pinocytosis is not applicable to red and white blood cells, however, because the cells are impermeable to trehalose.…”

mentioning

confidence: 99%

Trehalose transporter 1, a facilitated and high-capacity trehalose transporter, allows exogenous trehalose uptake into cells

Kikawada

Saito

Kanamori

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

227

211

View full text Add to dashboard Cite

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“…Trehalose, a disaccharide found in high concentrations in many desiccation-tolerant animals and plants (Crowe et al 1984Gadd et al 1987;Westh and Ramlov 1991;Bianchi et al 1993;Drennan et al 1993;Crowe and Crowe 2000), has been the lyoprotectant of choice for many cellular dehydration studies Wolkers et al 2001;Acker et al 2002) owing to its ability to replace the hydrogenbonded water molecules and depress the phase transition in dehydrated samples (Crowe and Crowe 1988;Harrigan et al 1990;Leslie et al 1994;Tsvetkova et al 1998).Trehalose also has a high glass transition temperature (T g ; Koster et al 1994Koster et al , 2000Sun et al 1996;Crowe et al 1997;Buitink et al 1998) owing to the stability of the glycosidic bond (Kacurakova and Mathlouthi 1996;Schebor et al 1999). The high glass transition state for trehalose allows living cells to be placed into a static glassy state at ambient temperature following the removal of cellular water.…”

Section: Anhydrobiosis and Cellular Injurymentioning

confidence: 99%

“…Several strategies to improve the mammalian cell's ability to take up trehalose have been reported, including a genetic engineering approach, in which a Staphylococcus α-haemolysin was used to porate mammalian fibroblasts and keratinocytes for trehalose uptake (Eroglu et al 2000), trehalose microinjection (Eroglu et al 2002) and thermal poration (Beattie et al 1997). It has recently been shown that mesenchymal stem cells can take up trehalose from the external environment by fluid phase endocytosis, reaching internal trehalose concentrations in the range 20-30 mm (Oliver et al 2004), which has been shown to protect human platelets during freeze-drying and storage (Wolkers et al 2001(Wolkers et al , 2002.…”

Section: Anhydrobiosis and Cellular Injurymentioning

confidence: 99%

“…The dehydrated storage of cells represents an alternative to cryopreservation and has already been shown to be effective for the storage of human blood platelets at room temperature for up to 2 years, during which time recovery and response to thrombin remained essentially unchanged (Wolkers et al 2001(Wolkers et al , 2002. Efforts to dry nucleated cells have also been reported (Guo et al 2000;Bloom et al 2001;Gordon et al 2001;Puhlev et al 2001;Acker et al 2002;Bhowmick et al 2003), but achieving consistent results of highly viable, physiologically active cells following dehydration to low water content remains elusive.…”

Section: Preservation Of Cells In the Dry Statementioning

confidence: 99%

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Dry storage of sperm: applications in primates and domestic animals

Meyers¹

2006

Reprod. Fertil. Dev.

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Abstract. Cryopreservation of spermatozoa, oocytes and embryos, as well as somatic cells or cell lines for cloning from cells, are all options for the long-term storage of unique genotypes and endangered species. Spermatozoal cryopreservation and storage currently require liquid nitrogen or ultralow refrigeration-based methods for longor short-term storage, which requires routine maintenance and extensive space requirements. The preservation of stem cells also has strict requirements for long-term storage to maintain genetic integrity. Dessicated (lyopreserved) sperm and stem cells will provide an unprecedented type of long-term storage without the need for expensive and burdensome cryogenic conditions. Experiments were conducted to determine an effective intracellular concentration of the lyoprotectant trehalose. High-pressure liquid chromatography studies revealed that trehalose can be incorporated into mature sperm cells as well as spermatogonial stem cells from rhesus monkeys. In addition, using fourier transform infrared spectroscopy, we determined that thermotropic phase transitions for fresh ejaculates from rhesus monkey and stallion sperm occurred at 10-15, 33-37 and 55-59 • C. Preliminary studies in our laboratory have indicated that spermatogonial stem cells can be dried to <3 g g −1 water and maintain viability following rehydration. Studies in our laboratory have provided preliminary results suggesting that the desiccated storage of sperm and spermatogonial stem cells may be a viable alternative to conventional cryopreservation.

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Drying: Biological Materials

Law

Mujumdar

2009

Encyclopedia of Industrial Biotechnology

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New advancements in biotechnology has made many biotechnological products such as yeast, bacteria, vitamins, enzymes, proteins, carbohydrates, antibiotics, fungi, lysine, blood, plasma, realize in industry and commercial market. Most of these products appear in the form of liquid, suspension, or paste, which require refrigeration for storage. Refrigeration however limits the widespread application of the products mentioned above, as the operating costs and handling of frozen products is high and difficult. As such, dehydration and drying can be deployed to reduce the handling cost, while prolonging the shelf life, retaining the active ingredients activity, maintaining the cell survival and vitality, as well as its physical, textural, sensory, and optical properties. Biotechnological products are heat sensitive. Conventional drying methods for nonbiotechnological products such as hot air drying may damage and denature the product, destroy the cell and active ingredients, cause case hardening and browning. Over the years, freeze‐drying is the most common method used in the drying of some of the biotechnological products mentioned above. However, the operating cost and the drying time of freeze‐drying are comparatively high. As oil prices are skyrocketing and there is no sign that the price will ease in the near future, researchers and scientists have designed, tested, and improved various drying methods in order to improve drying efficiency and performance. This article discusses various conventional drying methods, which are common to the drying of biotechnological products, and describes various advanced drying technologies that give improvements and better performance with reference to drying strategy, drying medium, handling of drying materials, and mode of heat input. These include intermittent drying, pulse combustion drying, impinging stream drying, cyclic pressure vacuum drying, spray‐freeze‐drying, atmospheric freeze‐drying, vacuum fluidized bed drying, low‐pressure spray drying, superheated steam drying, heat pump drying, inert medium drying, supercritical fluid drying, sorption drying, spouted bed drying, jet spouted bed drying, vibrating fluidized bed drying, pulse fluidized bed drying, high electric field drying, and microwave drying. Recent research findings of these drying technologies are given as well.

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Human Platelets Loaded with Trehalose Survive Freeze-Drying

Cited by 303 publications

References 18 publications

Trehalose transporter 1, a facilitated and high-capacity trehalose transporter, allows exogenous trehalose uptake into cells

Trehalose transporter 1, a facilitated and high-capacity trehalose transporter, allows exogenous trehalose uptake into cells

Dry storage of sperm: applications in primates and domestic animals

Drying: Biological Materials

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