Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

Steyer, Andrej; Poljšak-Prijatelj, Mateja; Barlič–Maganja, Darja; Marin, Jožica

doi:10.1099/vir.0.2008/001206-0

Cited by 118 publications

(98 citation statements)

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“…This observation supports the hypothesis that interspecies transmission of RVs, including to and from humans, might take place in nature (Cook et al, 2004;Gouvea et al, 1990;Palombo et al, 2000). Interspecies transmission could be the result of infection with an animal RV (Nakagomi & Nakagomi, 2002) or of genetic reassortment between human and animal RV strains during co-infection of the same cell (Das et al, 1993;Dunn et al, 1993;Urasawa et al, 1992;Varghese et al, 2004, Steyer et al, 2008Martella et al, 2006b). …”

Section: Lineage IVsupporting

confidence: 70%

Diversity of the G3 genes of human rotaviruses in isolates from Spain from 2004 to 2006: cross-species transmission and inter-genotype recombination generates alleles

Martı́nez-Laso

Román

Rodríguez

et al. 2009

Journal of General Virology

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Rotavirus evolves by using multiple genetic mechanisms which are an accumulation of spontaneous point mutations and reassortment events. Other mechanisms, such as crossspecies transmission and inter-genotype recombination, may be also involved. One of the most interesting genotypes in the accumulation of these events is the G3 genotype. In this work, six new Spanish G3 sequences belonging to 0-2-year-old patients from Madrid were analysed and compared with 160 others of the same genotype obtained from humans and other host species to establish the evolutionary pathways of the G3 genotype. The following results were obtained: (i) there are four different lineages of the G3 genotype which have evolved in different species; (ii) Spanish G3 rotavirus sequences are most similar to the described sequences that belong to lineage I; (iii) several G3 genotype alleles were reassigned as other G genotypes; and (iv) inter-genotype recombination events in G3 viruses involving G1 and G2 were described. These findings strongly suggest multiple inter-species transmission events between different non-human mammalian species and humans. INTRODUCTIONThe genus Rotavirus, in the family Reoviridae, is composed of seven different groups (groups A-G), although only groups A, B and C have been identified in humans (Hoshino & Kapikian, 1994). Group A rotavirus (RV) is responsible for most cases of gastroenteritis in humans and its genome consists of 11 double-stranded RNA (dsRNA) segments that encode six structural and six non-structural proteins. Two outer capsid proteins, VP4 and VP7, are used to define serotypes P (protease-cleaved protein VP4) and G (glycoprotein VP7) (Estes, 2001). RV serotypes are defined by virus neutralization, but genotypes are studied more often since they can be more easily characterized by sequencing or RT-PCR followed by sequencing of amplicons.Although 14 G serotypes, 16 G genotypes, 14 P serotypes and 27 P genotypes are known (Fukai et al., 2007;Gentsch et al., 1996;Liprandi et al., 2003;Martella et al., 2003;McNeal et al., 2005;Nakagomi et al., 1990), only 11 G and 15 P types have been identified in humans Martella et al., 2006a;McNeal et al., 2005;. Epidemiological studies in many parts of the world have indicated that there are five most common RV G types (G1, G2, G3, G4 and G9) and two P types (P[8] and P[4]) (Hoshino & Kapikian, 2000;Hoshino et al., 2002;Kapikian et al., 2001), and to a lesser extent P[6], P[9] and P[11] Santos et al., 2001), mainly found in countries of temperate climates. However, in countries of tropical and subtropical climates, other G and P types have been found to be most prevalent (Desselberger et al., 2001;Iturriza-Gó mara et al., 2003).There are a number of reports of RV strains isolated from humans and animals that share genetic and antigenic features of virus strains from heterologous species. In many cases, genetic analysis by hybridization has clearly demonstrated the genetic relatedness of gene segments from RV strains isolated from different species. Together with t...

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Section: Lineage IVsupporting

confidence: 70%

Diversity of the G3 genes of human rotaviruses in isolates from Spain from 2004 to 2006: cross-species transmission and inter-genotype recombination generates alleles

Martı́nez-Laso

Román

Rodríguez

et al. 2009

Journal of General Virology

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“…According to Martella et al (2010), research on genetic variability provides useful data for a better understanding of virus epidemiology, particularly with respect to evidence of inte rspecies transmission of rotaviruses (Steyer et al 2008, Mat thijnssens et al 2011, which increases the possibility of the emergence of new strains and unusual genotypic combinations. This type of research also underpins the development and monitoring of effective vaccines.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of group A bovine rotavirus in southeastern and central-western Brazil, 2009-2010

et al. 2012

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Rotavirus is an important cause of neonatal diarrhea in humans and several animal species, including calves. A study was conducted to examine 792 fecal samples collected from calves among 65 dairy and beef herds distributed in two of Brazil's major livestock producing regions, aiming to detect the occurrence of rotavirus and perform a molecular characterization of the rotavirus according to G and P genotypes in these regions. A total of 40 (5.05%) samples tested positive for rotavirus by the polyacrylamide gel electrophoresis (PAGE) technique. The molecular characterization was performed by multiplex semi-nested RT-PCR reactions, which indicated that the associations of genotypes circulating in herds in Brazil's southeastern region were G6P[11], G10P[11], G[-]P[5] + [11], G[-]P[6] in the state of São Paulo and G6P[11], G8P[5], G11P[11], G10P[11] in the state of Minas Gerais. In the central-western region, the genotypes G6P[5] + [11], G6P[5], G8P[-], G6P[11], G [-] P[1], G[-] P[11], and G[-] P[5] were detected in the state of Goiás, while the genotypes G6P[5], G8[P11], G6[P11], G8[P1], G8[P5], G6[P1] were circulating in herds in the state of Mato Grosso do Sul. The genotypic diversity of bovine rotavirus found in each region under study underlines the importance of characterizing the circulating samples in order to devise the most effective prophylactic measures.

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“…Although five G genotypes (G1 to G4, and G9) and three P genotypes (P [4], P [6], and P [8]) represent the majority of clinically important RVA strains in humans (36), the intensive RVA surveillance associated with introduction of the RVA vaccine into human populations has resulted in the detection of RVAs with unusual genotypes, including those commonly detected in pigs and cattle (14,19,21,29,38). Animal RVAs are therefore regarded as a potential reservoir for the genetic diversity of human RVAs, and consequently their ecology has been of great concern (24).…”

mentioning

confidence: 99%

Analysis of the Excretion Dynamics and Genotypic Characteristics of Rotavirus A during the Lives of Pigs Raised on Farms for Meat Production

et al. 2012

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To determine the excretion dynamics and genotypic characteristics of rotavirus A (RVA), a longitudinal observational study was performed in 10 pigs from 3 litters at a farrow-to-finish farm. A total of 400 fecal samples were directly collected from the rectums of individual pigs (aged 7 to 217 days) at 3- to 14-day intervals. Seventy-one samples (17.5%) were positive for RVA by reverse transcription-PCR designed to detect the VP7 and VP4 genes. At least 13 combinations of 5 G (G2, G4, G5, G9, and G11) and 6 P (P[6], P[7], P[13], P[23], P[27], and P[34]) genotypes were identified by direct sequencing of the PCR products. We were able to detect RVA VP7 sequences from each pig 4 to 6 times with intervals of 7 to 52 days (from 7 to 119 days of age). Each pig harbored RVAs with at least 3 to 6 different combinations of G and P genotypes, while repeated excretions of RVAs carrying the same combinations of G and P genotypes were also observed. Virus shedding and changes in G and P genotypes appeared to be associated with movement of the pigs into weaning, growing, and finishing barns. These results indicated that, over their lifetimes, pigs raised for meat frequently and intermittently excrete genetically diverse RVAs.

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Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment

Cited by 118 publications

References 50 publications

Diversity of the G3 genes of human rotaviruses in isolates from Spain from 2004 to 2006: cross-species transmission and inter-genotype recombination generates alleles

Diversity of the G3 genes of human rotaviruses in isolates from Spain from 2004 to 2006: cross-species transmission and inter-genotype recombination generates alleles

Molecular characterization of group A bovine rotavirus in southeastern and central-western Brazil, 2009-2010

Analysis of the Excretion Dynamics and Genotypic Characteristics of Rotavirus A during the Lives of Pigs Raised on Farms for Meat Production

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