Human pregnancies after transfer of fresh (four-to eight-cell) versus frozen-thawed blastocysts resulting from intracytoplasmic sperm injection

Servy, Edouard; Kaufmann, Robert A.; Liu, Z.; Ménézo, Yves; Keskintepe, Levent

doi:10.1007/bf02744935

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…Our earlier reports demonstrated that ICSI of frozen-thawed spermatozoa immobilized by aggressive abrasion of tails could fertilize mature bovine and caprine oocytes better than those injected intact [11,12]. Indeed, immobilization of sperm tails before human ICSI enhances success of this infertility treatment [14,31]. This has been attributed to faster sperm membrane dissolution and intermingling of the sperm nucleus with the oocyte's cytoplasm [32].…”

Section: Discussionmentioning

confidence: 97%

Bovine Blastocyst Development from Oocytes Injected with Freeze-Dried Spermatozoa1

Keskintepe

Pacholczyk

Machnicka

et al. 2002

Biology of Reproduction

124

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Pronuclear formation, and the chromosomal constitution and developmental capacity of bovine zygotes formed by intracytoplasmic sperm injection with freeze-dried (lyophilized) spermatozoa were evaluated. Frozen-thawed spermatozoa were selected, freeze-dried, and stored at 4 degrees C until use. After 22-24 h of in vitro maturation oocytes were denuded and injected singly with a lyophilized spermatozoon. Injected oocytes were activated by treatment with 10 microM ionomycin (5 min) alone and in combination with 1.9 mM 6-dimethylaminopurine (DMAP) for 4 h. Ionomycin plus DMAP activation treatment resulted in a significantly higher proportion of sperm-injected oocytes with two pronuclei than was found after activation with ionomycin alone (74% vs. 56%; P < 0.03). The rates of cleavage, morula, and blastocyst development of sperm-injected oocytes treated with ionomycin plus DMAP were higher than after activation with ionomycin alone (63.3%, 34.2%, and 29.6% vs. 44.7%, 18.7%, and 10.6%, respectively; P < 0.05). Seventy-three percent of blastocysts produced with lyophilized sperm were diploid. These results demonstrate that in vitro-matured bovine oocytes can be fertilized with freeze-dried sperm cells, and that resultant zygotes can develop into karyotypically normal blastocysts.

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Section: Discussionmentioning

confidence: 97%

Bovine Blastocyst Development from Oocytes Injected with Freeze-Dried Spermatozoa1

Keskintepe

Pacholczyk

Machnicka

et al. 2002

Biology of Reproduction

124

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Cryopreservation of bovine blastocysts obtained by intracytoplasmic sperm injection

Keskintepe

Brackett

2000

Theriogenology

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Culture and transfer of human blastocysts

Gardner

Schoolcraft

1999

Current Opinion in Obstetrics and Gynaecology

880

625

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The transfer of the human embryo at the blastocyst stage during an in-vitro fertilization procedure is a way of increasing implantation rates. This, in turn, means that significantly fewer embryos are required to be transferred in order to establish a successful pregnancy. The result of this is that high order multiple gestations are eliminated, while maintaining high pregnancy rates, in in-vitro fertilization.

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Human pregnancies after transfer of fresh (four-to eight-cell) versus frozen-thawed blastocysts resulting from intracytoplasmic sperm injection

Abstract: Purpose: The objective of this stud), was to obtain expanded blastocysts .following intracytoplasmic sperm injection (ICSI) and Vero-cell co-culture, cpyopreserve

Cited by 3 publications

References 23 publications

Bovine Blastocyst Development from Oocytes Injected with Freeze-Dried Spermatozoa1

Bovine Blastocyst Development from Oocytes Injected with Freeze-Dried Spermatozoa1

Cryopreservation of bovine blastocysts obtained by intracytoplasmic sperm injection

Culture and transfer of human blastocysts

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