Human preleukaemia cell culture studies in sideroblastic anaemia

Senn, J. S.; Pinkerton, P. H.; Price, Gerald B.; Mak, Tak W.; McCulloch, E. A.

doi:10.1038/bjc.1976.43

Cited by 24 publications

(2 citation statements)

References 15 publications

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“…The finding of leukaemic growth pattern preceding evolution of AML has also been reported in primary myelodysplasia (7, 13,14), where also pancytopenia was found predictive of leukaemic transformation (10) and correlated with short survival (6). The degree and number of cytopenias were not found predictive for outcome in our patients with secondary myelodysplasia.…”

Section: Discussionmentioning

confidence: 63%

Predictive value of bone marrow cultures in 48 patients with acute myeloblasts leukaemia or myelodysplasia both secondary to treatment of other malignant diseases

Mortensen

Pedersen‐Bjergaard

Pedersen

et al. 1985

Scandinavian Journal of Haematology

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The growth pattern of bone marrow cells in agar cultures was studied in 48 patients with acute myeloblasts leukaemia or myelodysplasia, secondary to cytoreductive treatment, and compared to other laboratory findings. At diagnosis the status of the patients was: acute myeloblastic leukaemia (AML) in 9, acute myeloproliferative syndrome (AMS) in 13 and preleukaemic syndrome (PL) in 26 patients. 20 of the 39 patients with AMS and PL developed acute leukaemia, 15 of them within 1 year. 15 died of complications to cytopenia and only 4 are still alive and without leukaemia, observed from 8 to 55 months. Progression to AML was predicted from the agar cultures by increased cluster growth. Among 18 patients with increased cluster growth at diagnosis 14 subsequently developed AML. Among 21 patients with normal or decreased cluster growth at diagnosis 6 patients developed AML, but after conversion of the growth pattern to increased cluster growth. In 2 patients the increased cluster growth disappeared spontaneously and the patients are long‐term leukaemia‐free survivors. Colony growth pattern, conversely, was not related to leukaemic progression. Only 1 patient had normal growth pattern at diagnosis with respect to both clusters and colonies. Survival was short, with a median of about 7 months, unrelated to growth pattern and leukaemic progression.

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Section: Discussionmentioning

confidence: 63%

Predictive value of bone marrow cultures in 48 patients with acute myeloblasts leukaemia or myelodysplasia both secondary to treatment of other malignant diseases

Mortensen

Pedersen‐Bjergaard

Pedersen

et al. 1985

Scandinavian Journal of Haematology

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“…The disorder may be slowly progressive, but once deterioration begins, outlook is limited and response to the usual chemotherapeutic agents is poor. For this reason, the disorder has been studied intensively, and abnormalities in cell culture have been noted (Greenberg et al, 1971;Senn & Pinkerton 1972;Senn et al, 1976). Linman & Bagby (1976) reported a favourable response in one preleukaemic patient which was predicted in cell culture.…”

Section: Discussionmentioning

confidence: 99%

Levamisole: evidence for activity on human haemopoietic progenitor cells

Senn¹,

Lai²,

Price³

1980

Br J Cancer

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Summary.-Levamisole, which has immunostimulant activity, is now being used to treat some forms of cancer. We report that the drug enhances granulocyte colony formation. The mechanism of action appears to be partly through modulation of molecules on cell membranes. The molecular content of colony-stimulating activity (CSA) released into leucocyte-conditioned medium by cells of leukaemic and some preleukaemic patients can be quantitatively altered by levamisole, both in vitro and in vivo, but the CSA produced is qualitatively similar to that released by normal cells. The demonstrated levamisole enhancement of colony formation, and altered CSA types detected in leucocyte-conditioned medium, makes this drug a promising candidate for treatment of selected leukaemic states and in preleukaemia.

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Normal and leukemic hemopoiesis compared

McCulloch¹,

Buick²,

Till³

1978

Cancer

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Using colony assays for human erythropoietic (BFU-E, CFU-E) and granulopoietic (CFU-C) progenitors, normal and leukemic myelopoietic differentiation were compared; similar patterns were found in both. However, the origin of blast cells characteristic of the disease could not be established indicating the need for a direct approach to these cells. A colony assay for blast cells in acute myeloblastic leukemia is described. Blast cell colony-formation is significantly correlated with blast cell number, and the colonies contain cells of blast like morphology without differentiation markers. It is proposed that this method, taken in conjunction with results from assays of myelopoiesis and lymphopoiesis, may provide a more complete picture of leukemic differentiation. It is anticipated that such a model will be useful in devising new therapies.

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Human preleukaemia cell culture studies in sideroblastic anaemia

Cited by 24 publications

References 15 publications

Predictive value of bone marrow cultures in 48 patients with acute myeloblasts leukaemia or myelodysplasia both secondary to treatment of other malignant diseases

Predictive value of bone marrow cultures in 48 patients with acute myeloblasts leukaemia or myelodysplasia both secondary to treatment of other malignant diseases

Levamisole: evidence for activity on human haemopoietic progenitor cells

Normal and leukemic hemopoiesis compared

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