Human Primary Endothelial Cells Stimulate Human Osteoprogenitor Cell Differentiation

Guillotin, Bertrand; Bourget, Chantal; Remy-Zolgadri, Murielle; Bareille, Reine; Fernandez, Philippe; Conrad, Véronique; Amédée-Vilamitjana, Joëlle

doi:10.1159/000080342

Cited by 130 publications

(92 citation statements)

References 30 publications

(38 reference statements)

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“…41 Besides that, ECs are pivotal members of a complex interactive communication network in bone. 42 Therefore, the chemical composition as well as architecture of combined structures was designed envisioning not only bone-forming cells but also ECs. Type I collagen, together with SPCL, is one of the building blocks of this scaffold and is the major constituent of the extracellular matrices to which proliferating ECs are exposed in injured tissue.…”

Section: Discussionmentioning

confidence: 99%

Design of Nano- and Microfiber Combined Scaffolds by Electrospinning of Collagen onto Starch-Based Fiber Meshes: A Man-Made Equivalent of Natural Extracellular Matrix

Tuzlakoğlu

Santos

Neves

et al. 2011

Tissue Engineering Part A

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Mimicking the structural organization and biologic function of natural extracellular matrix has been one of the main goals of tissue engineering. Nevertheless, the majority of scaffolding materials for bone regeneration highlights biochemical functionality in detriment of mechanical properties. In this work we present a rather innovative construct that combines in the same structure electrospun type I collagen nanofibers with starchbased microfibers. These combined structures were obtained by a two-step methodology and structurally consist in a type I collagen nano-network incorporated on a macro starch-based support. The morphology of the developed structures was assessed by several microscopy techniques and the collagenous nature of the nanonetwork was confirmed by immunohistochemistry. In addition, and especially regarding the requirements of large bone defects, we also successfully introduced the concept of layer by layer, as a way to produce thicker structures. In an attempt to recreate bone microenvironment, the design and biochemical composition of the combined structures also envisioned bone-forming cells and endothelial cells (ECs). The inclusion of a type I collagen nano-network induced a stretched morphology and improved the metabolic activity of osteoblasts. Regarding ECs, the presence of type I collagen on the combined structures provided adhesive support and obviated the need of precoating with fibronectin. It was also importantly observed that ECs on the nano-network organized into circular structures, a three-dimensional arrangement distinct from that observed for osteoblasts and resembling the microcappillary-like organizations formed during angiogenesis. By providing simultaneously physical and chemical cues for cells, the herein-proposed combined structures hold a great potential in bone regeneration as a man-made equivalent of extracellular matrix.

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Section: Discussionmentioning

confidence: 99%

Design of Nano- and Microfiber Combined Scaffolds by Electrospinning of Collagen onto Starch-Based Fiber Meshes: A Man-Made Equivalent of Natural Extracellular Matrix

Tuzlakoğlu

Santos

Neves

et al. 2011

Tissue Engineering Part A

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“…In addition to osteoblasts, GJIC is also involved in the expression of alkaline phosphatase and type I collagen in bone marrow stromal cells when co-cultured with endothelial cells (51). A subsequent study from this group found that alkaline phosphatase activity is only increased by the direct contact of human osteoprogenitor cells with human vascular endothelial cell types, while a transformed human bone marrow endothelial cell line has no such effect (60). Disruption of GJIC attenuates this osteogenic effect.…”

Section: Gap Junctions In Osteoblast Differentiation and Bone Formationmentioning

confidence: 99%

Roles of gap junctions and hemichannels in bone cell functions and in signal transmission of mechanical stress

Jiang¹,

2007

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Gap junctions formed by connexins (Cx) play an important role in transmitting signals between bone cells such as osteoblasts and osteoclasts, cells responsible for bone formation and bone remodeling, respectively. Gap junction intercellular communication (GJIC) has been demonstrated to mediate the process of osteoblast differentiation and bone formation. Furthermore, GJIC propagates Ca 2+ signaling, conveys anabolic effects of hormones and growth factors, and regulates gene transcription of osteoblast differentiation markers. GJIC is also implicated to regulate osteoclast formation, survival and apoptosis. Compared with other bone cells, the most abundant type are osteocytes, which express large amounts of connexins. Mechanosensing osteocytes connect and form gap junctions with themselves and other cells only through the tips of their dendritic processes, a relatively small percent of the total cell surface area compared to other cells. Recent studies show that in addition to gap junctions, osteoblasts and osteocytes express functional hemichannels, the un-opposed halves of gap junction channels. Hemichannels are localized at the cell surface and function independently of gap junctions. Hemichannels in osteocytes mediate the immediate release of prostaglandins in response to mechanical stress. The major challenges remaining in the field are how the functions of these two types of channels are coordinated in bone cells and what the asserted, distinct effects of these channels are on bone formation and remodeling processes, and on conveying signals elicited by mechanical loading. KeywordsBone; Gap Junctions; Hemichannels; Cx43; Connexin; Osteoblast; Osteocyte; Mechanical Stress; Review INTRODUCTION Gap Junctions in Bone CellsGap junctions are transmembrane channels, which connect the cytoplasm of adjacent cells. These channels permit molecules with molecular weights approximately less than 1 kD a such as small metabolites, ions, and intracellular signaling molecules (i.e. calcium, cAMP, inositol triphosphate) to pass through. Gap junction channels have been demonstrated to be important in modulating cell signaling and tissue function in many organs, such as heart, liver, peripheral nerve, ovary, ear and lens of the eye (1-8). Gap junctions are formed by members of a family of sequentially and structurally related proteins known as connexins. Approximately twenty connexins have been identified and cloned from various tissues and cells (9-11). Six monomers of connexins are joined head-to-head across the extracellular "gap" between two adjacent cells (21,26,27). However, we found that Cx45 protein is expressed in bone marrow, but not in osteoblasts, osteocytes and osteoclasts in the alveolar bone tissues of the tooth (28).Functional gap junctions in osteoblasts were first demonstrated with electrical conductance and dye injection (29). Voltage-sensitive gap junction currents were detected in osteoblastic cells derived from calvarias of new-born rats with a single gap junction channel conductance of approxim...

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“…8 Still unclear is the precise role of endothelial cells on osteoblastic differentiation, but emerging studies have demonstrated that coculture of endothelial cells and mesenchymal precursor/stem cells stimulated the expression of bone phenotypic markers and enhanced the osteogenesis of cocultured mesenchymal cells. [9][10][11] Recently, we reported that the ordered fluorapatite (OR-FA) surface when used as a potential implant coating, showed good biocompatibility with different cell lines, that is, dental pulp stem cells and osteoblast-like MG-63 cells and supported the long-term growth and differentiation of these cells. [12][13][14] This OR-FA surface stimulated, in vitro, the expression of a set of pro-osteogenic transcripts and bone mineralization phenotypic markers of ASCs, and consistently accelerated and enhanced in vivo mineralized tissue formation.…”

mentioning

confidence: 99%

Fluorapatite Enhances Mineralization of Mesenchymal/Endothelial Cocultures

Wang

Zhang

Chang

et al. 2014

Tissue Engineering Part A

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In addition to the widely used mesenchymal stem cells (MSCs), endothelial cells appear to be a favorable cell source for hard tissue regeneration. Previously, fluorapatite was shown to stimulate and enhance mineralization of MSCs. This study aims to investigate the growth of endothelial cells on synthesized ordered fluorapatite surfaces and their effect on the mineralization of adipose-derived stem cells (ASCs) through coculture. Endothelial cells were grown on fluorapatite surfaces and characterized by cell counting, flow cytometry, scanning electron microscopy, and enzyme-linked immunosorbent assay (ELISA). Cells were then cocultured with ASCs and stained for alkaline phosphatase and mineral formation. Fibroblast growth factor (FGF) pathway perturbation and basic FGF (bFGF) treatment of the ASCs were also conducted to observe their effects on differentiation and mineralization of these cells. Fluorapatite surfaces showed good biocompatibility in supporting endothelial cells. Without a mineralization supplement, coculture with endothelial cells induced osteogenic differentiation of ASCs, which was further enhanced by the fluorapatite surfaces. This suggested a combined stimulating effect of endothelial cells and fluorapatite surfaces on the enhanced mineralization of ASCs. Greater amounts of bFGF release by endothelial cells alone or cocultures with ASCs stimulated by fluorapatite surfaces, together with FGF pathway perturbation and bFGF treatment results, suggested that the FGF signaling pathway may function in this process.

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Human Primary Endothelial Cells Stimulate Human Osteoprogenitor Cell Differentiation

Cited by 130 publications

References 30 publications

Design of Nano- and Microfiber Combined Scaffolds by Electrospinning of Collagen onto Starch-Based Fiber Meshes: A Man-Made Equivalent of Natural Extracellular Matrix

Design of Nano- and Microfiber Combined Scaffolds by Electrospinning of Collagen onto Starch-Based Fiber Meshes: A Man-Made Equivalent of Natural Extracellular Matrix

Roles of gap junctions and hemichannels in bone cell functions and in signal transmission of mechanical stress

Fluorapatite Enhances Mineralization of Mesenchymal/Endothelial Cocultures

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