Human Primordial Germ Cell-Like Cell Induction from Pluripotent Stem Cells by SOX17 and PRDM1 Expression

Irie, Naoko; Kobayashi, Toshihiro; Azim Surani, M.

doi:10.1007/978-1-0716-3698-5_7

Methods in Molecular Biology

2024

DOI: 10.1007/978-1-0716-3698-5_7

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Human Primordial Germ Cell-Like Cell Induction from Pluripotent Stem Cells by SOX17 and PRDM1 Expression

Naoko Irie,

Toshihiro Kobayashi,

M. Azim Surani

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2024

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Cited by 2 publications

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What we can learn from the bovine embryo and mouse models to enable in vitro gametogenesis in cattle

Denicol

2024

Reprod. Fertil. Dev.

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The development of in vitro gametogenesis (IVG) in the mouse opened up unforeseen possibilities for assisted reproduction. The development of this technology to be used in cattle production could accelerate the rate of genetic selection by dramatically reducing the generation interval, while decreasing the environmental impact of livestock production as the need to grow animals in the process of genetic selection would be reduced or even eliminated. Although several steps of the process of IVG such as in vitro oocyte maturation and fertilization, and embryo production are already routinely performed in cattle, other steps of the system such as in vitro follicle and oocyte development are still rudimentary. The stable derivation of bovine pluripotent stem cells is the starting point without which IVG cannot be realized. However, producing a primordial germ cell and taking this cell through oogenesis and folliculogenesis in a dish will require a more detailed understanding of the milestones that need to be accomplished in vivo before they can be recapitulated in vitro. In particular, understanding the regulatory circuitry of germ cell specification in the embryo, the timing and events related to development of the germ cell program, and the factors necessary to make a competent egg, will need to be uncovered. Here, we review the process of IVG and provide a brief description of the current advances and bottlenecks related to in vitro oogenesis and folliculogenesis in cattle. Finally, we provide a brief comparison between mice and cows in this regard.

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What we can learn from the bovine embryo and mouse models to enable in vitro gametogenesis in cattle

Denicol

2024

Reprod. Fertil. Dev.

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The Influence of Markers in the Differentiation Process of Stem Cells Into Endothelial Cells to Support Treatment Testing Experiments

Nurdiyati

2024

JSCRTE

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Research on stem cells, particularly their differentiation into endothelial cells, is highly significant in the field of biomedical science and regenerative therapy. Endothelial cells are crucial for blood vessel formation, wound healing, tissue regeneration, and the treatment of degenerative diseases. Human pluripotent stem cells can differentiate into various cell types, making them valuable for repairing or replacing damaged tissue. This study reviews the role of markers in distinguishing human stem cells into endothelial cells. A comprehensive literature search was conducted, and out of 428 screened articles, only 4 met the inclusion criteria. SOXF proteins were analyzed using scRNA-seq analysis, focusing on their role in enhancing stem cell differentiation. SOX17 was found to significantly increase the percentage of cells expressing CD34+ and Vascular Endothelial Cadherin (VEC), consistent with its known role in endoderm differentiation and endothelial cell specification. SOX17 can override pluripotency signals in human stem cells, triggering their differentiation into endothelial cells. Overexpression of SOX17 in human stem cells resulted in cells with endothelial characteristics, and combining SOX17 with FGF2 enhanced this effect, resulting in more than 90% of cells expressing endothelial stem cell markers (CD34+, VEC+, CD31+). SOXF was applied to prompt stem cell differentiation, with only SOX17 demonstrating notable effectiveness.

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Human Primordial Germ Cell-Like Cell Induction from Pluripotent Stem Cells by SOX17 and PRDM1 Expression

Cited by 2 publications

References 25 publications

What we can learn from the bovine embryo and mouse models to enable in vitro gametogenesis in cattle

What we can learn from the bovine embryo and mouse models to enable in vitro gametogenesis in cattle

The Influence of Markers in the Differentiation Process of Stem Cells Into Endothelial Cells to Support Treatment Testing Experiments

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