Human recombinant cementum attachment protein (hrPTPLa/CAP) promotes hydroxyapatite crystal formation in vitro and bone healing in vivo

Aligned hydroxyapatite nano-crystal formation on a polyamide surface

“…[5][6][7][8] The general methods for controlling crystal growth involve proteins in mineralization systems in vitro. [9][10][11][12] However, the frangibility of proteins limits their applications in chemical synthesis. Surfactants, such as polyvinyl alcohol (PVA), sodium dodecyl sulfate (SDS), Tween, and cetyltrimethyl ammonium bromide, are also widely used for the control of apatite mineralization.…”

Identification and Qualitative Analysis of β-Sitosterol and Some Phytoestrogens in In Vivo and In-Vitro Samples of Lepidium sativum: A Semi-Arid Bone Healing Plant

“…Results obtained by HPTLC for diazdzein and formononetin were coincided with the results of [17][18][19]. Although originally identified in cementum, PTPLa/ CAP is very effective at inducing bone repair and healing and therefore this novel molecule has a great potential to be used for mineralized tissue bioengineering and tissue regeneration studied by [20]. Lepidium sativum seeds have been used in traditional folk medicine to heal fractured bones especially in cases of glucocorticoilds induced osteoporosis [21,22].…”

Cementum protein 1‐derived peptide (CEMP 1‐p1) modulates hydroxyapatite crystal formation in vitro

“…The role of CEMP1‐p1 on calcium phosphate crystal formation was performed in a cell‐free system. Controlled chemical reaction between the calcium and phosphate ions (without organic matrix) in a semisolid medium at physiological pH and RT was performed as described elsewhere . Briefly, semisolid crystallizing medium was prepared by using a sodium metasilicate (SMS) solution of specific gravity 1.06 g/ml, 1 M H 3 PO 4 , HEPES buffer 10mM, pH 7.2.…”

“…Controlled chemical reaction between the calcium and phosphate ions (without organic matrix) in a semisolid medium at physiological pH and RT was performed as described elsewhere. 19 Briefly, semisolid crystallizing medium was prepared by using a sodium metasilicate (SMS) solution of specific gravity 1.06 g/ml, 1 M H 3 PO 4 , HEPES buffer 10mM, pH 7.2. CEMP1-p1 peptide solutions (20 μg/mL) were prepared in 10 mM HEPES pH 7.4 and mixed with the SMS.…”