Human recombinant interleukin-2125 induced in vitro proliferation of equine, caprine, ovine, canine and feline peripheral blood lymphocytes

Fenwick, B. W.; Schore, C. E.; Osburn, Bennie

doi:10.1016/0147-9571(88)90008-2

Cited by 19 publications

(4 citation statements)

References 45 publications

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“…In previous study, it has been reported that rhIL-2 induces the proliferation of PBL from several animals species including canine [7,11,29]. The proliferation of canine lymphocytes by concanavalin A (Con A), phytohemagglutinin or pokeweed mitogen, which are T cell mitogen in human, has also demonstrated [19].…”

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confidence: 99%

A Novel Culture Method of Canine Peripheral Blood Lymphocytes with Concanavalin A and Recombinant Human Interleukin-2 for Adoptive Immunotherapy

et al. 2007

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ABSTRACT. This study was designed to develop a novel culture method for the efficient proliferation of canine peripheral blood lymphocytes (cPBL) for adoptive immunotherapy. When cPBL were cultured in the presence of concanavalin A (Con A), proliferation of cPBL was induced and expression of interleukin-2 receptor (IL-2R) which enables to respond to exogenously added IL-2 was upregulated. And then, when cPBL were cultured with recombinant human interleukin-2 (rhIL-2) in addition to Con A, proliferation was accelerated and increased to about 10-fold after 1 week. The phenotypic analysis showed that the main population of the cultured cPBL was consisted of CD8+ positive lymphocytes. Among them, CD4+CD8+ double positive (DP) lymphocytes had significantly increased, and the ratio of CD4+ single positive (SP) lymphocytes to CD8+ SP lymphocytes (CD4+SP/CD8+SP) was decreased as compared to before culturing. To evaluate the cytotoxic activity of cPBL cultured with Con A and rhIL-2, furthermore, cytotoxic assay was carried out against xenogeneic melanoma cell line (MeWo), which resulted in MHC-unrestricted cytokilling. These results suggest that the culture method of cPBL by the use of Con A and rhIL-2 may be useful for generating lymphokine activated killer cells, and also this may be beneficial for adoptive immunotherapy of tumor-bearing dogs.

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confidence: 99%

A Novel Culture Method of Canine Peripheral Blood Lymphocytes with Concanavalin A and Recombinant Human Interleukin-2 for Adoptive Immunotherapy

et al. 2007

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“…Also, the activity of feline IL-2 can be partially blocked by monoclonal antibody to human IL-2 [29]. The similarities of feline and human IL-2 are further supported by the findings that feline lymphocytes are responsive to human IL-2 [74] and large granular feline cytotoxic lymphocytes are induced by human IL-2 in vitro [71]. Also, infected cats given human IL-2 in combination with 3'-azido-3'-deoxythymidine (AZT) resist challenge with feline leukemia virus [75].…”

Section: (3) Interleukinmentioning

confidence: 69%

Feline immune system

Lin

1992

Comparative Immunology, Microbiology and Infectious Diseases

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“…These results indicate that hrIL-2 can enhance the proliferative response of activated pony lymphocytes. It has been shown that this lymphokine induced in a dose dependent manner the proliferation of unstimulated lymphocytes of horse (FENWICK et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

Production of Interleukin 2 and Expression of Interleukin 2 Receptors by Pony Peripheral Blood Lymphocytes after Stimulation with a Soluble Fraction of Trypanosoma evansi

Ahmed

Schmid

Hörchner

1990

Journal of Veterinary Medicine, Series B

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Summary Pony peripheral blood lymphocytes (PBL) were stimulated with a soluble fraction of Trypanosoma (T.) evansi (SF). As determined by 3H‐thymidine incorporation, the cells underwent a proliferative response and were able to: a) produce a factor having the biological activities of interleukin 2 (IL‐2) since their supernatants could support the in vitro growth of pony PBL stimulated with concanavalin A (Con A‐blasts); b) undergo a further proliferative response when incubated in short term cultures with SF, human recombinant IL‐2 (hrIL‐2), or both c) bind specifically radiolabelled hrIL‐2 (125I‐hrIL‐2). The date described here indicate that a soluble fraction of T. evansi stimulated pony PBL which subsequently produced IL‐2 and expressed IL‐2 receptors (IL‐2R).

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Human recombinant interleukin-2125 induced in vitro proliferation of equine, caprine, ovine, canine and feline peripheral blood lymphocytes

Cited by 19 publications

References 45 publications

A Novel Culture Method of Canine Peripheral Blood Lymphocytes with Concanavalin A and Recombinant Human Interleukin-2 for Adoptive Immunotherapy

A Novel Culture Method of Canine Peripheral Blood Lymphocytes with Concanavalin A and Recombinant Human Interleukin-2 for Adoptive Immunotherapy

Feline immune system

Production of Interleukin 2 and Expression of Interleukin 2 Receptors by Pony Peripheral Blood Lymphocytes after Stimulation with a Soluble Fraction of Trypanosoma evansi

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