Human recombinant monoamine oxidase B as reliable and efficient enzyme source for inhibitor screening

Inhibition of monoamine oxidase by selected C5- and C6-substituted isatin analogues

“…For this purpose the recombinant human MAO-A and MAO-B enzymes were employed. 28 Kynuramine is non-fluorescent until undergoing MAO-catalysed oxidative deamination and subsequent ring closure to yield 4-hydroxyquinoline, a fluorescent metabolite. The concentrations of the MAO-generated 4-hydroxyquinoline in the incubation mixtures was determined by comparing the fluorescence emitted by the samples to that of known amounts of authentic 4-hydroxyquinoline.…”

Synthesis of Amide and Ester Derivatives of Cinnamic Acid and Its Analogs: Evaluation of Their Free Radical Scavenging and Monoamine Oxidase and Cholinesterase Inhibitory Activities

“…MAO Inhibitory Assay MAO inhibitory activity was assayed using the method of Novaroli et al 36) with minor modifications. Briefly, 140 µL of 0.1 M potassium phosphate buffer (pH 7.4), 8 µL of 0.75 mM kynuramine, and 2 µL of DMSO inhibitor solution (final DMSO concentration of 1% v/v) were preincubated at 37°C for 10 min.…”

A Scaffold‐Tree‐Merging Strategy for Prospective Bioactivity Annotation of γ‐Pyrones

“…[16] Following pre-screens at fixed concentration, IC 50 values were measured to identify hit candidates (see the Supporting Information for details, including Figure 6). For MAO A, the assay [17] yielded 60 inhibitors with IC 50 values of 10 mm or lower; 15 of these inhibitors had an IC 50 value less than 1 mm. For MAO B, 35 inhibitors with IC 50 values below 10 mm and 11 inhibitors with IC 50 values below 1 mm were identified.…”