Human red blood cell membrane potential and fluidity in glucose solutions

Заводник, И. Б.; Piasecka, Anna; Szosland, Konrad; Bryszewska, Maria

doi:10.3109/00365519709057819

Cited by 26 publications

(14 citation statements)

References 12 publications

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“…4 ). This is of particular relevance, in light of the fact, that erythrocytes have a membrane potential close to 0 mV 56 , 57 . Our data thus suggest that in erythrocytes the percentage of PIEZO1 channels available for activation could change from 5% in normal individuals to more than 40% in individuals carrying a single-point mutation (Fig.…”

Section: Discussionmentioning

confidence: 96%

Voltage gating of mechanosensitive PIEZO channels

et al. 2018

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Mechanosensitive PIEZO ion channels are evolutionarily conserved proteins whose presence is critical for normal physiology in multicellular organisms. Here we show that, in addition to mechanical stimuli, PIEZO channels are also powerfully modulated by voltage and can even switch to a purely voltage-gated mode. Mutations that cause human diseases, such as xerocytosis, profoundly shift voltage sensitivity of PIEZO1 channels toward the resting membrane potential and strongly promote voltage gating. Voltage modulation may be explained by the presence of an inactivation gate in the pore, the opening of which is promoted by outward permeation. Older invertebrate (fly) and vertebrate (fish) PIEZO proteins are also voltage sensitive, but voltage gating is a much more prominent feature of these older channels. We propose that the voltage sensitivity of PIEZO channels is a deep property co-opted to add a regulatory mechanism for PIEZO activation in widely different cellular contexts.

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Section: Discussionmentioning

confidence: 96%

Voltage gating of mechanosensitive PIEZO channels

et al. 2018

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“…DPH is localized in hydrophobic region near the center of the bilayer, while TMA–DPH is incorporated into the polar region of the erythrocyte membrane. The increase in fluorescence anisotropy indicates that the membrane becomes more rigid (less fluid) [17]. …”

Section: Methodsmentioning

confidence: 99%

Oxidative damages in erythrocytes of patients with metabolic syndrome

et al. 2013

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The aim of the study was to estimate the changes caused by oxidative stress in structure and function of membrane of erythrocytes from patients with metabolic syndrome (MS). The study involved 85 patients with MS before pharmacological treatment and 75 healthy volunteers as a control group. Cholesterol level, lipid peroxidation, glutathione level (GSH), and antioxidant enzyme activities in erythrocytes were investigated. The damage to erythrocyte proteins was also indicated by means of activity of ATPase (total and Na+,K+ ATPase) and thiol group level. The membrane fluidity of erythrocytes was estimated by the fluorescent method. The cholesterol concentration and the level of lipid peroxidation were significantly higher, whereas the concentration of proteins thiol groups decreased in the patient group. ATPase and GSH peroxidase activities diminished compared to those in the control group. There were no differences in either catalase or superoxide dismutase activities. The membrane fluidity was lower in erythrocytes from patients with MS than in the ones from control group. These results show changes in red blood cells of patients with MS as a consequence of a higher concentration of cholesterol in the membrane and an increased oxidative stress.

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“…These elevated levels of glucose can result in an enhanced osmotic fragility of red blood cells (RBCs), in membrane lipid peroxidation and in changed morphological properties [3,4].…”

Section: Introductionmentioning

confidence: 99%