Human red cell hemolysis rates in the subsecond to seconds range. An analysis

Anderson, Philip C.; Lovrien, Rex

doi:10.1016/s0006-3495(77)85543-4

Cited by 25 publications

(13 citation statements)

References 2 publications

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“…-~ 8o: It is well known that the haemolysis of the erythrocytes has a typical sigmoidal shape with increasing the NaC1 concentration in the external medium of the cells [1,2]. Our data are in accord with the data presented so far in the literature, the gamma irradiated erythrocytes as well as the unirradiated erythrocytes exhibited a similar typical sigmoidal shape with the increasing NaC1 concentration in the external medium of the cells.…”

Section: Results and Conclusionsupporting

confidence: 90%

Effect of microwaves, high energy electrons and gamma irradiation on the osmotic resistance of human erythrocyte membranes

et al. 1999

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The effects of 5 MeV electrons and of gamma irradiation at 0 ~ as well as the effects of 2.45 GHz microwaves at -196 ~ on the osmotic resistance of human erythrocyte membranes are presented. Both electron and gamma radiation in the range 0-400 Gy induced no haemolysis, indicating that the membrane modifications due to radiation interaction do not reach a critical point to cause swelling of the cells and consequent lysis. The microwave irradiated erythrocytes showed a haemolysis degree between 6-16% in the irradiation time range of 0-5 min. The osmotic stress experiments performed after irradiation showed that the gamma irradiated erythrocytes exhibited an almost similar behaviour at all irradiation doses whereas the electron irradiated samples showed a much larger increase in the haemolysis degree, and in the case of a certain electron dose (100 Gy), the haemolysis was found much smaller than for the control sample. A similar behaviour of the erythrocytes was found in the case of microwave irradiation of the cryoprotected erythrocytes at -196~ the sample irradiated for 6 min developed a haemolysis degree much smaller than for the control (70% from the nonirradiated sample haemolysis). Our experimental data suggest that electron radiation and gamma radiation have different impacts on the erythrocyte haemolysis in the dose range used. Much more experimental data is necessary in order to clarify the similarity between the results obtained in the case of electron irradiation at 0 ~ and microwave irradiation at -196 ~ in the two different sets of irradiation conditions (liquid samples at 0 ~ and frozen samples at -196 ~ IntroductionThe impact of high energy electrons on the biological systems is very rarely treated in the published literature [5]. The aim of this paper is to study the differences in the impacts of 5 MeV electrons and gamma radiation, at 0 ~ as well as the effects of 2.45 GHz microwaves at -196 ~ on the osmotic properties of human erythrocyte membranes. The relative rate ofhaemolysis is a well known parameter accounting for the osmotic properties of the erythrocyte membranes [2]. Haemolysis is the process of pore forming in the erythrocyte membrane which allows to release haemoglobin into the external medium of the cells. Radiation studies performed with X rays suggested that radiation induced haemolysis is an osmotic haemolysis, due to the partial destruction of the membrane permeability [6]. In our work, the modifications of the erythrocyte membranes properties were estimated by measuring the haemolysis induced by radiation in the samples and the haemolysis induced experimentally by osmotic stress on the irradiated erythrocytes.

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Section: Results and Conclusionsupporting

confidence: 90%

Effect of microwaves, high energy electrons and gamma irradiation on the osmotic resistance of human erythrocyte membranes

et al. 1999

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“…The two-phase release of hemoglobin described here for a transient osmotic pulse with PBS diluent has also been described for hypotonic lysis by Anderson and Lovrien (1977). In the first phase, the shortest time to initial lysis, obtained with lysis by water, was 0.6 sec.…”

Section: Discussionmentioning

confidence: 77%

Effect of inositol hexaphosphate on the transient behavior of red cells following a DMSO‐induced osmotic pulse

Franco

Barker

Novick

et al. 1986

Journal Cellular Physiology

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An osmotic pulse can be used to incorporate inositol hexaphosphate (IHP) into red cells. The pulse is induced by equilibrating a red cell suspension with DMSO and then rapidly diluting with an isotonic IHP solution. Since IHP binds to hemoglobin and lowers the affinity for oxygen, this method may find application in the preparation of low-affinity cells for experimental and clinical use. The experiments reported here examined the dynamic changes of several red cell variables immediately following the osmotic pulse. The effect of IHP, which has been shown to dissociate red cell cytoskeletons, was evaluated by comparison with a matched phosphate-buffered saline (PBS) diluent. Red cell morphology, volume, and hemoglobin permeability were studied by fixing the cells at times ranging from 0.06 to 300 sec after dilution. Mechanical fragility was measured by subjecting the cells to a short period of shear stress at the same times after dilution. With both diluents, the cells underwent a rapid increase in volume followed by a return towards normal volume with a maximum at less than 250 msec. With IHP diluent, the period of hemoglobin permeability immediately followed the size peak and was completed by about 1 sec after dilution. PBS also induced a second leakage at longer times (10-120 sec), which resulted in a morphological dichotomy with ghosts and intact cells. The choice of diluent also affected sensitivity to shear stress. The IHP-treated cells had a mechanical fragility maximum at about 1 sec. The PBS-treated cells exhibited no enhanced mechanical fragility. An unexpected result was the inhibition of the second phase of lysis in PBS-treated cells by a properly timed shear stress.

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“…The abundance of unsaturated fatty acids (Raz and Livne 1973;Shand and Noble 1981;Vajreswari et al 1983) was shown to be related to a greater extent of membrane expansion when cells are exposed to hypotonic solutions. This increase in membrane surface allows an increase in cellular critical volume which is positively correlated with the osmotic resistance (Anderson and Lovrien 1977) when the resulting hemolysis is studied as a function of salt concentration.…”

Section: Discussionmentioning

confidence: 99%

The effect of dietary lipids on the trout erythrocyte membrane

Leray

Nonnotte

1986

Fish Physiol Biochem

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Rainbow trout were fed either a commercial diet or semi-purified diets containing 8% by weight of either cod liver oil, grape seed oil or hydrogenated coconut oil. Important changes in the fatty acid composition of erythrocyte membrane phospholipids were induced by these dietary fats. No changes were seen in the cholesterol/phospholipid ratio and in the cellular ATP level. Shape changes (crenation of cell margin and shrinkage) were only seen in trout fed hydrogenated coconut oil in connection with an accumulation of high amounts of (n-9) fatty acids including 20:3 (n-9) phospholipids. The compositional changes affect the stability of the erythrocyte membrane. An increased rate of osmotic hemolysis appeared to be associated with an increased unsaturated fatty acid content of the membranes.

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Human red cell hemolysis rates in the subsecond to seconds range. An analysis

Cited by 25 publications

References 2 publications

Effect of microwaves, high energy electrons and gamma irradiation on the osmotic resistance of human erythrocyte membranes

Effect of microwaves, high energy electrons and gamma irradiation on the osmotic resistance of human erythrocyte membranes

Effect of inositol hexaphosphate on the transient behavior of red cells following a DMSO‐induced osmotic pulse

The effect of dietary lipids on the trout erythrocyte membrane

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