The study investigated the relationship between workload and lecturers' job satisfaction in Adekunle Ajasin University, Akungba Akoko, Ondo State. The descriptive research design of the survey type was employed. The sample of the study was 105 lecturers selected using multi-stage sampling technique across three faculties. Two research questions and three hypotheses were formulated to guide the study. A researcher-made questionnaire titled "Workload and Lecturers' Job Satisfaction Questionnaire (WLJSQ)" was used to elicit information from the respondents. The instrument was validated by experts in Test and Measurement and the test re-test method was used for test reliability which yielded a reliability coefficient of 0.72. The findings of this study revealed: lecturers found conducting research, processing of results, marking of examination scripts and supervision of undergraduate projects most demanding and that lecturers' job satisfaction was low in relations with the workload. Also, there is a significant relationship between marking of examination scripts and lecturers' job satisfaction, supervision of research work and lecturers' job satisfaction and there is a significant relationship between number of courses allocated and lecturers' job satisfaction. It is hereby recommended that more lecturers should be recruited particularly in faculties with high student population in order to reduce excessive workload, while the number of students to be allocated to lecturers for project supervision should be reduced and the services of assistant lecturers should be employed to assist in the area of marking of scripts and supervision of undergraduate projects.