2008
DOI: 10.1164/rccm.200708-1243oc
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Human Rhinovirus 1B Exposure Induces Phosphatidylinositol 3-Kinase–dependent Airway Inflammation in Mice

Abstract: Rationale: Infection with rhinovirus (RV) triggers exacerbations of asthma and chronic obstructive lung disease. Objectives: We sought to develop a mouse model of RV employing RV1B, a minor group serotype that binds to the low-density lipoprotein receptor. Methods: C57BL/6 mice were inoculated intranasally with RV1B, replication-deficient ultraviolet (UV)-irradiated RV1B, or RV39, a major group virus. Measurements and Main Results: Viral RNA was present in the lungs of RV1B-treated mice, but not in those expos… Show more

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Cited by 123 publications
(209 citation statements)
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“…Thus, it is conceivable that, in addition to increasing paracellular permeability, RV facilitates secondary bacterial infection of the airway epithelium by attenuating mucociliary clearance. Although this cannot easily be tested in an in vitro model, we developed an animal model of RV infection in which mice show persistence of viral RNA up to 4 days, mild neutrophilic lung inflammation, lung interferon production, and airways cholinergic hyperresponsiveness (27).…”
Section: Discussionmentioning
confidence: 99%
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“…Thus, it is conceivable that, in addition to increasing paracellular permeability, RV facilitates secondary bacterial infection of the airway epithelium by attenuating mucociliary clearance. Although this cannot easily be tested in an in vitro model, we developed an animal model of RV infection in which mice show persistence of viral RNA up to 4 days, mild neutrophilic lung inflammation, lung interferon production, and airways cholinergic hyperresponsiveness (27).…”
Section: Discussionmentioning
confidence: 99%
“…C57BL/6 mice were inoculated intransally with RV1B (TCID 50 , 5 3 10 7 / mouse) or equal volume of sham, as previously described (27). After 24 hours, mice were killed and lungs were perfused with ethylenediamine tetraacetic acid (EDTA), inflated and fixed in formalin, and embedded in paraffin.…”
Section: Inoculation Of Micementioning
confidence: 99%
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“…Moreover, RV1B, a minor receptor subtype, was capable of binding the mouse receptor and inducing successful infection in vivo; increased airway inflammation and hyperresponsiveness has also been documented. Phosphatidylinositol 3-kinase/Akt kinase signalling was required for maximal RV1B-induced neutrophilic inflammation, probably via its essential role in virus internalisation [74].…”
Section: Mechanisms Of Virus-induced Exacerbationmentioning
confidence: 98%
“…Airway resistance was measured as described (14). Mice were anesthesized, intubated, and ventilated with a Buxco FinePointe operating system (Wilmington, NC).…”
Section: Analysis Of Airway Resistancementioning
confidence: 99%