Human Salivary Histatin-1 Is More Efficacious in Promoting Acute Skin Wound Healing Than Acellular Dermal Matrix Paste

Lei, Xiaoxuan; Cheng, Liuhanghang; Lin, Haiyan; Pang, Ming; Yao, Zexin; Chen, Caihong; Forouzanfar, Tymour; Bikker, Floris J.; Wu, Gang

doi:10.3389/fbioe.2020.00999

Cited by 17 publications

(13 citation statements)

References 52 publications

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“…In addition to contraction, collagen secretion is one of the important functions of fibroblasts. Previous studies have also proved that Hst 1 can promote the density of collagen in the wound local site 18 . Myofibroblasts, which can express α‐SMA, transformation from fibroblasts are the main source of extra cellular matrix (ECM) proteins during wound healing 27,28 .…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have also proved that Hst 1 can promote the density of collagen in the wound local site. 18 Myofibroblasts, which can express α‐SMA, transformation from fibroblasts are the main source of extra cellular matrix (ECM) proteins during wound healing. 27 , 28 In the process of wound healing, insufficient collagen production will seriously affect the formation of ECM and granulation tissue, thus delaying wound healing.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have also proved that Hst 1 can promote the density of collagen in the wound local site. 18…”

Section: Discussionmentioning

confidence: 99%

“…In addition to oral epithelial cells, Hst 1 can activate many other cells 16,17 . Recent studies have also shown that Hst 1 can inhibit the expression of interleukin‐1β (IL‐1β), C‐reactive protein (CRP) and CD68 in wound healing 18 . However, there is no study about the mechanical properties of the healed skin after treatment with Hst 1.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies suggested that this was mainly related to its promotion of epithelial cell migration, 20 while ignoring the contraction effect of myofibroblasts. Although some experiments tested the effect of Hst 1 on collagen deposition, 18 , 21 they did not study the mechanical properties of the skin after wound healing or proved the effect of fibroblasts in vitro. In this study, the effects of Hst 1 on the characteristics and function of fibroblasts were studied completely in vivo and in vitro, and we tried to explore the mechanism of the effect.…”

Section: Introductionmentioning

confidence: 99%

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Histatin 1 enhanced the speed and quality of wound healing through regulating the behaviour of fibroblast

et al. 2021

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Objectives Histatin 1(Hst 1) has been proved to promote wound healing. However, there was no specific study on the regulation made by Hst 1 of fibroblasts in the process of wound healing. This research comprehensively studied the regulation of Hst 1 on the function of fibroblasts in the process of wound healing and preliminary mechanism about it. Materials and methods The full‐thickness skin wound model was made on the back of C57/BL6 mice. The wound healing, collagen deposition and fibroblast distribution were detected on days 3, 5 and 7 after injury. Fibroblast was cultured in vitro and stimulated with Hst 1, and then, their biological characteristics and functions were detected. Results Histatin 1 can effectively promote wound healing, improve collagen deposition during and after healing and increase the number and function of fibroblasts. After healing, the mechanical properties of the skin also improved. In vitro, the migration ability of fibroblasts stimulated by Hst 1 was significantly improved, and the fibroblasts transformed more into myofibroblasts, which improved the function of contraction and collagen secretion. In fibroblasts, mTOR signalling pathway can be activated by Hst 1. Conclusions Histatin 1 can accelerate wound healing and improve the mechanical properties of healed skin by promoting the function of fibroblasts. The intermolecular mechanisms need to be further studied, and this study provides a direction about mTOR signalling pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Previous studies have also proved that Hst 1 can promote the density of collagen in the wound local site. 18…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Histatin 1 enhanced the speed and quality of wound healing through regulating the behaviour of fibroblast

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Cellular and Molecular Mechanisms of Healing of Cutaneous and Oral Mucosal Wounds

Ibrahim Korkmaz,

Gibbs

2024

Pathological Basis of Oral and Maxillofacial Diseases

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Multifunctional Silk Fibroin Methacryloyl Microneedle for Diabetic Wound Healing

Guan

Zhang

Jiang

et al. 2022

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of diabetes mellitus. Globally, these wounds are important causes of disability and mortality. [2] Diabetic wounds that fail to heal are largely caused by oxidative stress. [3] Overproduction of reactive oxygen species (ROS) and inadequate antioxidant protection results in excess oxidative stress, leading to cell apoptosis and suppressed cell proliferation as well as differentiation at diabetic wound sites. [4] In high glucose (HG) conditions, glucotoxicity disrupts angiogenesis, thereby limiting the transport and delivery of nutrients and oxygen in diabetic wounds. [5] Diabetic skin wounds are more susceptible to bacterial infections, causing belated healing and life-threatening outcomes. [6,7] Bacterial infections are also involved in oxidative stress induction. [8] Excess oxidative stress levels suppress growth factor-mediated signaling pathways, resulting in dysregulated angiogenesis. Oxidative stress, angiogenesis, and bacterial infections have been reported to jointly cause diabetic wound healing disorders. [9] The existing therapies include drugs or application of bio-activators, hyperbaric oxygen therapy, and stem cell therapy. [10][11][12] However, these therapies are expensive, single-functional, immature, and cumbersome to operate in many cases. [13,14] The limited efficacies create an urgent need for the development of novel treatment modalities.Microneedle (MN) patches are emerging minimally-invasive local delivery systems that have the ability to overcome the dosing inefficiency of systemic delivery. And the new-generation MN, such as bioinspired or stimuli-responsive MN, exhibits more individualized, more efficient, and more convenient. [15,16] These patches have been applied for the treatment of various diseases, including skin wounds. [17,18] However, the current MN patches cannot act on multiple targets of diabetes wound pathogenesis. [19] We developed a multifunctional MN patch with the ability to simultaneously target oxidative stress, angiogenesis, and bacterial infections in diabetic wounds. A silk fibroin methacryloyl hydrogel was selected as the base material for multifunctional MN patches. Silk fibroin methacryloyl was formed from silk fibroin, a physiologically inactive protein derived from Bombyx mori silkworm cocoon, modified with glycidyl-methacrylate groups to supply a cross-linkage site essential for photopolymerization. Silk fibroin methacryloyl has excellent biocompatibility, biodegradability, stable mechanical Diabetic wound is one of the common complications in diabetic patients, which exhibits chronic, hard-to-heal characteristics. The healing process of wounds is impaired by several factors, including excessive oxidative stress, blocked angiogenesis, and bacterial infection. The therapeutic effects of traditional microneedle patches remain not satisfactory, due to their difficulty simultaneously targeting multiple targets to treat diabetic wounds. As such, there is an urgent need to develop a multifunctional microneedle (MN) patch for promoting the healing of diabetic wounds. ...

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Human Salivary Histatin-1 Is More Efficacious in Promoting Acute Skin Wound Healing Than Acellular Dermal Matrix Paste

Cited by 17 publications

References 52 publications

Histatin 1 enhanced the speed and quality of wound healing through regulating the behaviour of fibroblast

Histatin 1 enhanced the speed and quality of wound healing through regulating the behaviour of fibroblast

Cellular and Molecular Mechanisms of Healing of Cutaneous and Oral Mucosal Wounds

Multifunctional Silk Fibroin Methacryloyl Microneedle for Diabetic Wound Healing

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