Human Serologic Response to Envelope-Associated Proteins and Adenylate Cyclase Toxin of Bordetella pertussis

Abstract

The human serologic response to several envelope-associated proteins and adenylate cyclase toxin of Bordetella pertussis was examined using immunoblot techniques. Antigens recognized by sera from individuals with culture-confirmed pertussis and by sera from infants immunized with three doses of conventional whole-cell pertussis vaccine included a 63,000-Da protein that was shown to be antigenically related to a mycobacterial heat-shock protein. A 29,000-Da protein reacted with sera from convalescent individuals, whereas a 91,000-Da protein reacted with sera from vaccinated individuals. Antibodies to adenylate cyclase toxin were common in sera from individuals diagnosed with pertussis. B. pertussis lipooligosaccharide was also recognized by antibodies in some of these sera. These data suggest that some of these antigens may play a role in immunity to pertussis.

Monospecific antibody against Bordetella pertussis Adenylate Cyclase protects from Pertussis

“…No doubt, the current acellular pertussis vaccines prevent against full bloom disease, substantially exposed individuals become infected with B.pertussis and develop a milder form of the disease for which new vaccination strategies are under investigation 29 and here we suggest new formulation also. Significant human humoral antibody response to B.pertussis AC reported by Farfel et al 15 and Arciniega et al 30 and other published literature 16,[23][24][25] supports our findings to include other antigens such as AC, in present formulation of DtaP because reports reveal that the efficacy of the various DTaP vaccines was not optimal in five trials and DTwP vaccines were more effective than DTaP vaccines. 31,32 Therefore, it is the high time to review the antibody responses of DTaP to other antigens including AC and ascertain their role for inclusion in DTaP along with PT, PRN and FHA.…”

Laboratory Diagnosis of Pertussis

“…However, responses were low or even absent in children with pertussis who had been vaccinated with WCV or ACV (211), and vaccination with WCV or 5-component ACV did not induce IgG-ACT (211,212). The authors suggested that the poor IgG-ACT response in vaccinated children can be explained by a preferential response to antigens for which they were primed, a phenomenon known as original antigenic sin (213).…”

Cyclic AMP-Mediated Suppression of Neutrophil Extracellular Trap Formation and Apoptosis by the Bordetella pertussis Adenylate Cyclase Toxin

“…Addition of new antigens to the vaccine has been suggested as a solution to this resurgence (2). A leading candidate for inclusion in future acellular vaccine formulations is the adenylate cyclase toxin (ACT) because it is essential for virulence, immunogenic, and a demonstrated protective antigen (3)(4)(5)(6). ACT is composed of a 400-amino-acid C-terminal catalytic domain and a 1,306-amino-acid cell-binding domain that is homologous to the repeats in toxins (RTX) family of pore-forming bacterial protein toxins (7).…”