Human serum albumin as a probe for surface conditioning (opsonization) of block copolymer-coated microspheres

Norman, Maria E.; Williams, Paul; Illum, Lisbeth

doi:10.1016/0142-9612(92)90177-p

Cited by 82 publications

(31 citation statements)

References 27 publications

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“…For example, the amount of HSA adsorbed has been found to increase with increasing surface hydrophobicity. Norman et al have observed that more HSA is adsorbed onto hydrophobic polystyrene particles than those coated with hydrophilic poloxamer or poloxamine block copolymers [20], following the same trend as macrophage uptake [21,22]. Indeed, the group [20] was able to use HSA to probe opsonization of the particles coated with various polymers.…”

Section: Introductionmentioning

confidence: 84%

“…HSA, and human monocytes and polymorphonuclear leukocytes (PMNLs). As aforementioned, HSA is the most abundant serum protein and its adsorption has been connected with in vitro phagocytosis and the prediction of in vivo fate of particles [20]. Although IgG exhibits responsive adhesion to PNIPAm nanoparticles [38], this protein usually binds to speci c receptors on phagocytic cells via the Fc portion [41].…”

Section: Introductionmentioning

confidence: 99%

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In vitro studies of the interaction of poly(NIPAm/MAA) nanoparticles with proteins and cells

Moselhy

Nicholov

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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The pH- and temperature-responsive poly(N-isopropylacrylamide-co-methacrylic acid) (PNIPAm/MAA) nanoparticles are of potential application in targeted drug delivery. Their responsive properties in the presence of human serum albumin were investigated using dynamic light scattering (DLS), protein assay, and electron spin resonance (ESR) spectroscopy. Their interaction with human monocytes and polymorphonuclear leukocytes (PMNLs) was studied using scanning electron microscopy (SEM) and oxygen consumption method. The nanoparticles exhibited a volume phase transition at 35-40 degrees C in Hanks balanced salt solution (HBSS) and in phosphate buffer solution (PBS) of pH 7.4. The diameter of the nanoparticles decreased slightly in the presence of HSA at 25 degrees C at neutral pH, whereas an increase in the diameter in pH 6 PBS at 40 degrees C was revealed. The amount of albumin adsorbed onto the nanoparticles decreased with increasing temperature. The ESR spectra of spin labeled HSA indicated a more restricted environment in the nanoparticles at elevated temperatures. The stimulation of PMNL oxygen consumption by PNIPAm based nanoparticles, an indication of phagocytosis of the particles, was not observed regardless whether the nanoparticles were incubated in plasma or serum. In contrast, the more hydrophobic polystyrene (PSt) particles induced a significant increase in the rate of oxygen consumption after the incubation. PNIPAm/MAA-grafted-PSt particles behaved similarly to the PNIPAm/MAA nanoparticles, suggesting that surface properties dictate the recognition of colloids by PMNLs.

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Section: Introductionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

In vitro studies of the interaction of poly(NIPAm/MAA) nanoparticles with proteins and cells

Moselhy

Nicholov

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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“…In the case of polymeric nanoparticles, although negative charge can be related to a higher uptake [6,31], the surface hydrophobicity appears to be the key factor for opsonization. Nanoparticles prepared from hydrophobic polymers PSt [32], PLA [33], PLGA [34] and PACA [35] undergo important adsorption of Ig, complement proteins and other plasma proteins like albumin, either in vitro or in vivo. To account for these observations, a higher level of protein adsorption on hydrophobic surfaces than on hydrophilic ones has been proposed [36], as well as high affinity of IgG and albumin for hydrophobic regions [28] (van der Waals interactions may be a more accurate description for these ''hydrophobic'' interactions [37]).…”

Section: Surface Propertiesmentioning

confidence: 99%

Nanocarriers’ entry into the cell: relevance to drug delivery

Hillaireau

Couvreur

2009

Cell. Mol. Life Sci.

1,360

1,015

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Nanocarriers offer unique possibilities to overcome cellular barriers in order to improve the delivery of various drugs and drug candidates, including the promising therapeutic biomacromolecules (i.e., nucleic acids, proteins). There are various mechanisms of nanocarrier cell internalization that are dramatically influenced by nanoparticles' physicochemical properties. Depending on the cellular uptake and intracellular trafficking, different pharmacological applications may be considered. This review will discuss these opportunities, starting with the phagocytosis pathway, which, being increasingly well characterized and understood, has allowed several successes in the treatment of certain cancers and infectious diseases. On the other hand, the non-phagocytic pathways encompass various complicated mechanisms, such as clathrin-mediated endocytosis, caveolae-mediated endocytosis and macropinocytosis, which are more challenging to control for pharmaceutical drug delivery applications. Nevertheless, various strategies are being actively investigated in order to tailor nanocarriers able to deliver anticancer agents, nucleic acids, proteins and peptides for therapeutic applications by these non-phagocytic routes.

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“…The number of proteins bound to the particles increased with increasing hydrophobicity of the particle surface, as well as with increasing size. Generally, hydrophobic particles are opsonized more quickly than hydrophilic particles, due to the enhanced absorbability of plasma proteins onto the surface of hydrophobic particles [86][87][88][89]. The roles of particle size, surface curvature, and particle surface area in protein binding have also been investigated.…”

Section: Surface Hydrophobicity Evaluation Of Dna Gel Particlesmentioning

confidence: 99%

DNA gel particles: An overview

Morán

Vinardell

Infante

et al. 2014

Advances in Colloid and Interface Science

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A general understanding of interactions between DNA and oppositely charged compounds forms the basis for developing novel DNA-based materials, including gel particles. The association strength, which is altered by varying the chemical structure of the cationic cosolute, determines the spatial homogeneity of the gelation process, creating DNA reservoir devices and DNA matrix devices that can be designed to release either single-(ssDNA) or double-stranded (dsDNA). This review covers recent developments on the topic of DNA gel particles formed in water-water emulsion-type interfaces. The degree of DNA entrapment, particle morphology, swelling/dissolution behaviour and DNA release responses are discussed as a function of the nature of the cationic agent used. On the basis of designing DNA gel particles for therapeutic purposes, recent studies on the determination of the surface hydrophobicity, the haemolytic and the cytotoxic assessments of the obtained DNA gel particles have been also reported.

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Human serum albumin as a probe for surface conditioning (opsonization) of block copolymer-coated microspheres

Cited by 82 publications

References 27 publications

In vitro studies of the interaction of poly(NIPAm/MAA) nanoparticles with proteins and cells

In vitro studies of the interaction of poly(NIPAm/MAA) nanoparticles with proteins and cells

Nanocarriers’ entry into the cell: relevance to drug delivery

DNA gel particles: An overview

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