Human serum antibody response toHelicobacter pyloriwhole cell antigen in an institutionalized Bangladeshi population

Abstract

Aims: To use a commercial ELISA kit and an immunoblot assay to investigate the antibody levels of selected members of the Bangladeshi population to Helicobacter pylori protein antigens. Methods and Results: Using immunoblotting, high seroprevalence rates were observed in all age groups, although the subjects within the 1±9 years age group had the highest seroprevalence of antibodies to H. pylori antigens. By ELISA, the highest level of seroprevalence was observed in those over the age of 20 years. Conclusions: On the basis of these results the overall prevalence rate of H. pylori infection for the whole population was 77Á4%; 77Á9% for orphan boys and 76% for carers. CagA antibodies were detected in 86% of those with high levels of antibodies to H. pylori antigens. Signi®cance and Impact of the Study: A combination of immunoblotting and ELISA was the most ef®cient means of detecting serum antibodies to H. pylori antigens and could be applied to the screening of human sera for H. pylori-speci®c antibodies.

DNA-Level Characterization ofHelicobacter pyloriStrains from Patients with Overt Disease and with Benign Infections in Bangladesh

“…An age-related prevalence study in Bangladesh showed that 61% of infants (1 to 3 months old) were positive for H. pylori, and the figure rose to 84% in 6-to 9-yearolds (22). Otherwise, the reported seroprevalence of H. pylori in the hospitalized Bangladeshi population was 77.4% (26). A high association of H. pylori with PU (duodenal ulcer, 77%; gastric ulcer, 75%) and gastritis (74%) was observed in a previous study (12).…”

The Role of Environmental Reservoirs in Human Campylobacteriosis

“…are capable of zoonotic transfer through the faecal-oral route and have been reported to be unable to multiply outside warm-blooded host animals [9,12,13]. They colonise the intestinal mucosa of all food-producing animals and humans.…”

Immune Response to Helicobacter pylori

“…pylori induce a strong specific systemic and local antibody response and infected individual had antibodies against whole bacteria or part of it [49]and increase in plasma cells in gastric mucosa which produce IgA [50]. Other important antibody was IgG that binds to H. pylori and enhance phagocytosis [51].This antibodies lead to complement activation by either classical or alternative pathways [52].…”