Human serum promotes the proliferation but not the stemness genes expression of human adipose-derived stem cells

Hui, Chua Kien; Safwani, Wan Kamarul Zaman Wan; Chin, Seah Shiao; Malek, Annisaa Abu Samah Abdul; Hassan, Noormazita; Fazil, Muhamad Syakeer; Rooshdi, Raja Abdul Wafy Raja Muhammad; Hamid, Adila A.; Sathappan, Somasundaram

doi:10.1007/s12257-012-0354-1

Cited by 6 publications

(7 citation statements)

References 26 publications

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“…Since serum is the physiologic fluid formed at the wound site at the beginning of the healing, during the coagulation process, we first evaluated the effect of a human platelet-free serum on in vitro proliferation and differentiation of hASC in comparison with FBS, obtained from whole bovine blood and considered the gold standard as medium supplement for hASC cultures. We found that HS sustained in vitro hASC expansion with a growth rate significantly higher than FBS, in agreement with other literature data (Kocaoemer et al, 2007; Hui et al, 2012; Witzeneder et al, 2013). As reported also by Kocaoemer et al (2007), no significant differences were observed in the number of CFU-f isolated in the presence of HS or FBS, thus confirming that the higher number of cells obtained with HS as medium supplement was due to a higher proliferation rate of the cells and not to a higher number of recruited progenitors.…”

Section: Discussionsupporting

confidence: 93%

Platelet Lysate Activates Human Subcutaneous Adipose Tissue Cells by Promoting Cell Proliferation and Their Paracrine Activity Toward Epidermal Keratinocytes

Romaldini

Mastrogiacomo

Cancedda

et al. 2018

Front. Bioeng. Biotechnol.

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Skin chronic wounds are non-healing ulcerative defects, which arise in association with a morbidity state, such as diabetes and vascular insufficiency or as the consequence of systemic factors including advanced age. Platelet Rich Plasma, a platelet-rich blood fraction, can significantly improve the healing of human skin chronic ulcers. Given that the subcutaneous adipose tissue is located beneath the skin and plays a role in the skin homeostasis, in this study, we investigated the in vitro response of human subcutaneous adipose tissue cells to platelet content in a model mimicking in vitro the in situ milieu of a deep skin injury. Considering that, at the wound site, plasma turn to serum, platelets are activated and inflammation occurs, human adipose-derived stromal cells (hASC) were cultured with Human Serum (HS) supplemented or not with Platelet Lysate (PL) and/or IL-1α. We observed that HS sustained hASC proliferation more efficiently than FBS and induced a spontaneous adipogenic differentiation in the cells. PL added to HS enhanced hASC proliferation, regardless the presence of IL-1α. In the presence of PL, hASC progressively lessened the adipogenic phenotype, possibly because the proliferation of less committed cells was induced. However, these cells resumed adipogenesis in permissive conditions. Accordingly, PL induced in quiescent cells activation of the proliferation-related pathways ERK, Akt, and STAT-3 and expression of Cyclin D1. Moreover, PL induced an early and transient increase of the pro-inflammatory response triggered by IL-1α, by inducing COX-2 expression and secretion of a large amount of PGE2, IL-6, and IL-8. Media conditioned by PL-stimulated hASC exerted a chemotactic activity on human keratinocytes and favored the healing of an in vitro scratch wound. In order to bridge the gap between in vitro results and possible in vivo events, the stimuli were also tested in ex vivo cultures of in toto human adipose tissue biopsies (hAT). PL induced cell proliferation in hAT and outgrowth of committed progenitor cells able to differentiate in permissive conditions. In conclusion, we report that the adipose tissue responds to the wound microenvironment by activating the proliferation of adipose tissue progenitor cells and promoting the release of factors favoring wound healing.

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Section: Discussionsupporting

confidence: 93%

Platelet Lysate Activates Human Subcutaneous Adipose Tissue Cells by Promoting Cell Proliferation and Their Paracrine Activity Toward Epidermal Keratinocytes

Romaldini

Mastrogiacomo

Cancedda

et al. 2018

Front. Bioeng. Biotechnol.

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“…8,9 Recently, human adipose-derived stem cells (ADSCs) have aroused tremendous research interest as alternative sources of MSCs primarily because of their ease of isolation, extensive proliferation ability, and hypoimmunogenic nature. 10 Unlike MSCs, they represent an abundant source of pluripotent stem cells that can be easily isolated from subcutaneous adipose tissue through minimally invasive procedures such as Taken together with all these advantages, the use of ADSCs as an alternative stem cell source for various biomedical applications holds great promise.…”

Section: Introductionmentioning

confidence: 99%

Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces

Tan¹,

Tay²,

Chua³

et al. 2014

IJN

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Two important criteria of an ideal biomaterial in the field of stem cells research are to regulate the cell proliferation without the loss of its pluripotency and to direct the differentiation into a specific cell lineage when desired. The present study describes the influence of TiO 2 nanofibrous surface structures on the regulation of proliferation and stemness preservation of adipose-derived stem cells (ADSCs). TiO 2 nanofiber arrays were produced in situ onto Ti-6Al-4V substrate via a thermal oxidation process and the successful fabrication of these nanostructures was confirmed by field emission scanning electron microscopy (FESEM), energy dispersive spectrometer (EDS), X-ray diffractometer (XRD), and contact angle measurement. ADSCs were seeded on two types of Ti-6Al-4V surfaces (TiO 2 nanofibers and flat control), and their morphology, proliferation, and stemness expression were analyzed using FESEM, AlamarBlue assay, flow cytometry, and quantitative real-time polymerase chain reaction (qRT-PCR) after 2 weeks of incubation, respectively. The results show that ADSCs exhibit better adhesion and significantly enhanced proliferation on the TiO 2 nanofibrous surfaces compared to the flat control surfaces. The greater proliferation ability of TiO 2 nanofibrous surfaces was further confirmed by the results of cell cycle assay. More importantly, TiO 2 nanofibrous surfaces significantly upregulate the expressions of stemness markers Sox-2, Nanog3, Rex-1, and Nestin. These results demonstrate that TiO 2 nanofibrous surfaces can be used to enhance cell adhesion and proliferation while simultaneously maintaining the stemness of ADSCs, thereby representing a promising approach for their potential application in the field of bone tissue engineering as well as regenerative therapies.

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“…Human serum is a better alternative for cell culture because it is economical, safer and easily available. Human serum has been shown to promote ADSCs proliferation (Hui et al 2012), bone marrow derived stem cell growth (Shahdadfar et al 2005) and promote engraftment after allogenic transplantation of human peripheral blood haematopoetic stem cells in NOD/SCID mice (Kim et al 2005). In our study, ADSCs is characterised by flow cytometry analysis.…”

Section: Resultsmentioning

confidence: 93%

Chondrogenesis of Adipose-derived Stem Cells with Chondrocytes in Low Serum towards Clinical Application

Hamid¹,

Jeyabalan

Omar

et al. 2018

JSM

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Currently, fetal bovine serum (FBS) have been widely use in culture media to promote human cell proliferation. However, the usage of FBS for cell therapy in clinical application was associated with the risk of viral and prion transmission as well as immune rejection. To provide an option for this risk, this study was conducted to determine the effect of adipose derived stem cells (ADSCs) co-culture with chondrocyte in promoting cell proliferation and chondrogenesis toward FBS free condition. ADSCs co-cultured with chondrocyte at the ratio of 1:1, 2:1 and 1:2 were tested. Cell morphology changes, cell proliferation and gene expression level of stemness (Oct4, FGF-4, Nanog) and chondrogenic (Collagen Type II, ACP) were assessed. The results showed ADSCs in all co-culture groups changed morphology from fibroblastic spindle to polygonal shape which resembled chondrocytes. The morphological changes were accompanied with increased expression of chondrogenic genes; denoted chondrogenesis process. While maintaining expression of stemness genes indicated continuation of cell proliferation. From the three co-culture groups tested; ADSCs and chondrocytes (1:1 ratio) have been shown to exert better effects in promoting cell proliferation and chondrogenesis. In conclusion, ADSCs could replace FBS to grow sufficient number of chondrogenic cells to repair cartilage injury in the near future. Further in vivo study should be performed to test the effectiveness of this co-culture technique in cartilage injury repair.

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Human serum promotes the proliferation but not the stemness genes expression of human adipose-derived stem cells

Cited by 6 publications

References 26 publications

Platelet Lysate Activates Human Subcutaneous Adipose Tissue Cells by Promoting Cell Proliferation and Their Paracrine Activity Toward Epidermal Keratinocytes

Platelet Lysate Activates Human Subcutaneous Adipose Tissue Cells by Promoting Cell Proliferation and Their Paracrine Activity Toward Epidermal Keratinocytes

Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces

Chondrogenesis of Adipose-derived Stem Cells with Chondrocytes in Low Serum towards Clinical Application

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Human serum promotes the proliferation but not the stemness genes expression of human adipose-derived stem cells

Cited by 6 publications

References 26 publications

Platelet Lysate Activates Human Subcutaneous Adipose Tissue Cells by Promoting Cell Proliferation and Their Paracrine Activity Toward Epidermal Keratinocytes

Platelet Lysate Activates Human Subcutaneous Adipose Tissue Cells by Promoting Cell Proliferation and Their Paracrine Activity Toward Epidermal Keratinocytes

Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in&nbsp;situ TiO2 nanofibrous surfaces

Chondrogenesis of Adipose-derived Stem Cells with Chondrocytes in Low Serum towards Clinical Application

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Proliferation and stemness preservation of human adipose-derived stem cells by surface-modified in situ TiO2 nanofibrous surfaces