Human SGBS Cells &amp;ndash; a Unique Tool for Studies of Human Fat Cell Biology

Fischer‐Posovszky, Pamela; Newell, Felicity; Wabitsch, Martin; Törnqvist, Hans

doi:10.1159/000145784

Cited by 236 publications

(259 citation statements)

References 31 publications

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“…1). The gene expression changes were consistent with previously published data confi rming that SGBS preadipocyte differentiation is a reproducible model for the study of human adipogenesis ( 12,(37)(38)(39)(40).…”

Section: Lipid-quant Data Analysis and Outputsupporting

confidence: 90%

LipiD-QuanT: a novel method to quantify lipid accumulation in live cells

Varinli

Osmond

Molloy

et al. 2015

Journal of Lipid Research

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Section: Lipid-quant Data Analysis and Outputsupporting

confidence: 90%

LipiD-QuanT: a novel method to quantify lipid accumulation in live cells

Varinli

Osmond

Molloy

et al. 2015

Journal of Lipid Research

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“…lipid-loading machinery was analyzed in human SGBS (pre)adipocytes, a well-established adipocyte cell culture model (46), and in human primary adipocytes by quantitative RT-PCR. Several known components required for antigen loading (pro-saposin, NPC2, α-galactisodase) as well as CD1D itself were expressed at background levels in undifferentiated preadipocytes, but were readily detectable in mature human SGBS adipocytes and primary adipocytes ( Figure 6A).…”

Section: Figurementioning

confidence: 99%

“…The human preadipocyte SGBS cell line was cultured and differentiated into adipocytes as described previously (46,71). Full-length cDNA encoding human CD1d was cloned into a pLenti CMV vector (Addgene).…”

mentioning

confidence: 99%

Natural killer T cells in adipose tissue prevent insulin resistance

Bateman¹,

Rakhshandehroo²,

Graaf³

et al. 2012

J. Clin. Invest.

190

207

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Lipid overload and adipocyte dysfunction are key to the development of insulin resistance and can be induced by a high-fat diet. CD1d-restricted invariant natural killer T (iNKT) cells have been proposed as mediators between lipid overload and insulin resistance, but recent studies found decreased iNKT cell numbers and marginal effects of iNKT cell depletion on insulin resistance under high-fat diet conditions. Here, we focused on the role of iNKT cells under normal conditions. We showed that iNKT cell-deficient mice on a low-fat diet, considered a normal diet for mice, displayed a distinctive insulin resistance phenotype without overt adipose tissue inflammation. Insulin resistance was characterized by adipocyte dysfunction, including adipocyte hypertrophy, increased leptin, and decreased adiponectin levels. The lack of liver abnormalities in CD1d-null mice together with the enrichment of CD1d-restricted iNKT cells in both mouse and human adipose tissue indicated a specific role for adipose tissue-resident iNKT cells in the development of insulin resistance. Strikingly, iNKT cell function was directly modulated by adipocytes, which acted as lipid antigen-presenting cells in a CD1d-mediated fashion. Based on these findings, we propose that, especially under low-fat diet conditions, adipose tissue-resident iNKT cells maintain healthy adipose tissue through direct interplay with adipocytes and prevent insulin resistance.

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“…This cell line has been used with success by various investigators interested in human adipocyte biology (ϳ30 studies reviewed in Ref. 109). The exact molecular mechanism leading to the increased capacity to proliferate with unchanged ability to differentiate remains unknown.…”

Section: Human Preadipocyte Cell Line (Simpson-golabi-behmel Syndromementioning

confidence: 99%

Historical perspectives in fat cell biology: the fat cell as a model for the investigation of hormonal and metabolic pathways

Lafontan

2012

American Journal of Physiology-Cell Physiology

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Lafontan M. Historical perspectives in fat cell biology: the fat cell as a model for the investigation of hormonal and metabolic pathways. Am J Physiol Cell Physiol 302: C327-C359, 2012. First published September 7, 2011; doi:10.1152/ajpcell.00168.2011.-For many years, there was little interest in the biochemistry or physiology of adipose tissue. It is now well recognized that adipocytes play an important dynamic role in metabolic regulation. They are able to sense metabolic states via their ability to perceive a large number of nervous and hormonal signals. They are also able to produce hormones, called adipokines, that affect nutrient intake, metabolism and energy expenditure. The report by Rodbell in 1964 that intact fat cells can be obtained by collagenase digestion of adipose tissue revolutionized studies on the hormonal regulation and metabolism of the fat cell. In the context of the advent of systems biology in the field of cell biology, the present seems an appropriate time to look back at the global contribution of the fat cell to cell biology knowledge. This review focuses on the very early approaches that used the fat cell as a tool to discover and understand various cellular mechanisms. Attention essentially focuses on the early investigations revealing the major contribution of mature fat cells and also fat cells originating from adipose cell lines to the discovery of major events related to hormone action (hormone receptors and transduction pathways involved in hormonal signaling) and mechanisms involved in metabolite processing (hexose uptake and uptake, storage, and efflux of fatty acids). Dormant preadipocytes exist in the stroma-vascular fraction of the adipose tissue of rodents and humans; cell culture systems have proven to be valuable models for the study of the processes involved in the formation of new fat cells. Finally, more recent insights into adipocyte secretion, a completely new role with major metabolic impact, are also briefly summarized. history; adipocytes; insulin action; 3T3-L1 cells; glucose transport; fatty acids; lipolysis; catecholamines; preadipocytes SINCE THE EARLIEST TIMES, attention has been directed toward fat-related questions. It is currently accepted that the ability to store fat in adipose tissue was a major advantage for survival during cold winters and periods of food shortage for our "primitive" hunter ancestors. However, in modern societies, with increased food availability and nutritional efficacy, excessive fat deposition is frequently associated with degenerative or metabolic diseases. As early as 1901, life insurance companies noted that excess weight, particularly around the abdomen, was linked to reduced life expectancy. The risk was confirmed in the seminal studies of Vague (366) in Marseilles, who established the importance of abdominal (central) obesity in conferring excess mortality. The health hazard of obesity is not considered in the present review, which, rather, focuses on adipose tissue and adipocytes.

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Human SGBS Cells – a Unique Tool for Studies of Human Fat Cell Biology

Cited by 236 publications

References 31 publications

LipiD-QuanT: a novel method to quantify lipid accumulation in live cells

LipiD-QuanT: a novel method to quantify lipid accumulation in live cells

Natural killer T cells in adipose tissue prevent insulin resistance

Historical perspectives in fat cell biology: the fat cell as a model for the investigation of hormonal and metabolic pathways

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