Human sperm chemotaxis depends on critical levels of reactive oxygen species

Sánchez, Raúl; Sepúlveda, Carolina; Risopatrón, Jennie; Villegas, Jaime Eduardo Bernal; Giojalas, Laura Cecilia

doi:10.1016/j.fertnstert.2008.09.049

Cited by 23 publications

(14 citation statements)

References 18 publications

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“…The intermediate slowdown period is necessary for the sperm to recover the ability to respond appropriately to calcium ionophore/ionophores with the acrosome reaction, and this time coincides with the minimum response time for capacitation in the human sperm population. Similar results were also obtained with chemotaxis, another process associated with capacitation transformations and ROS production (Sánchez et al. , 2010).…”

Section: Discussionsupporting

confidence: 81%

Endogenous reactive oxygen species content and modulation of tyrosine phosphorylation during sperm capacitation

Donà¹,

Fiore

Tibaldi³

et al. 2011

Int J of Andrology

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Generation of controlled amounts of reactive oxygen species (ROS) and phosphorylation of protein tyrosine (Tyr) residues are two main cellular changes involved in sperm capacitation. This study examined the relationship between tyrosine-phosphorylation (Tyr-P) and endogenous ROS production during sperm capacitation, and correlated them with both sperm motility and functionality expressed as acrosome-reacted cells. Immediate ROS generation was observed to peak after a 45-min incubation, followed by a rapid decrease in ROS content and successive regeneration of the ROS peak in 3 h and later. These two peaks were directly correlated with both the Tyr-P process involving sperm heads and tails, and the acrosome reaction (69 ± 8% and 65 ± 4%, respectively). The period of low-ROS content resulted in low Tyr-P patterns, located exclusively in the cell midpiece, and drastic reduction in acrosome-reacted cells. Ascorbic acid addition inhibited both Tyr-P patterns and acrosome reactions, whereas NADPH induced high ROS generation, with Tyr-P patterns located only on sperm tails, and prevented the acrosome reaction. Sperm hyperactivation was insensitive to ROS content. This is an important parameter for evaluation of sperm capacitation, which is achieved only when both ROS generation reaches a peak and Tyr-P involves the sperm head.

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Section: Discussionsupporting

confidence: 81%

Endogenous reactive oxygen species content and modulation of tyrosine phosphorylation during sperm capacitation

Donà¹,

Fiore

Tibaldi³

et al. 2011

Int J of Andrology

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“…Oxidative stress control has a physiological impact on reproductive processes; low free radical levels modulate gamete function, particularly capacitation and chemotactic acquisition by spermatozoa (Sanchez et al ., ), sperm‐mediated oocyte activation, embryonic genome activation and blastocysts hatching from the zona pellucida (Harvey et al ., ), which are basic elements of fertilisation process. A supra‐physiological level of reactive oxygen species (ROS) has been associated with deleterious effects due to destruction or alteration of proteins, lipids and nucleic acids (Ma, ).…”

Section: Discussionmentioning

confidence: 99%

Supplementation of IVF medium with melatonin: effect on sperm functionality andin vitroproduced bovine embryos

et al. 2014

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Gamete co-incubation generates high free radical levels surrounding growing zygotes which may impair subsequent embryo viability. Melatonin eliminates a wide variety of free radicals; hence, we tried to improve in vitro embryo production by adding melatonin to in vitro fertilisation (IVF) media in high (Exp. 1) and low concentrations (Exp. 2), and we evaluated its effect on bull sperm function during IVF co-incubation time (Exp. 3). In Experiment 1, we supplemented IVF media culture with 0.01, 0.1 and 1 mmol of melatonin, along with a no melatonin control group. In Experiment 2, melatonin levels were reduced to 10, 100 and 1000 nmol, with a no melatonin control group. In Experiment 3, spermatozoa were incubated in IVF media with melatonin (as Exp. 2) and functional parameters were analysed at 0, 4 and 18 h. In Experiment 1, only 1 mmol melatonin showed lesser blastocyst rates than control (C: 23.2 ± 6.7% versus 1 mmol: 2.0 ± 1.7%). In Experiment 2, no statistical differences were found in cleavage percentage, blastocyst percentage and total cell count for any melatonin treatment. In Experiment 3, sperm samples with 1000 nmol melatonin had a significantly higher wobbler (WOB) coefficient, a lower percentage of intact acrosomes, a lower percentage of viable spermatozoa with ROS, greater DNA fragmentation and higher DNA oxidation than controls. Total fluorescence intensity for ROS at 10 nmol melatonin was significantly greater than controls (P < 0.05). IVF media with 1 mmol melatonin is deleterious for embryo development, and in lower concentrations, it modulated sperm functionality, but had no effects on embryo production.

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“…The results obtained suggest that a threshold of ROS must exist for normal chemotaxis to occur. High levels of ROS caused a state of oxidative stress leading to a lower percentage of chemotactic spermatozoa [11].…”

Section: Acrosome Reactionmentioning

confidence: 99%

“…Accumulating evidence suggests that low levels of ROS are of paramount importance in the activation of intracellular pathways responsible for spermatozoa maturation, capacitation, hyperactivation, acrosomal reaction, chemotactic processes as well as fusion with the female gamete [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Contemporary evidence on the physiological role of reactive oxygen species in human sperm function

Plessis

Agarwal

Halabi

et al. 2015

J Assist Reprod Genet

212

155

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Reactive oxygen species (ROS) play an important role in male fertility. Overproduction of reactive oxygen species (ROS) has been associated with a variety of male fertility complications, including leukocytospermia, varicocele and idiopathic infertility. The subsequent oxidative insult to spermatozoa can manifest as insufficient energy metabolism, lipid peroxidation and DNA damage, leading to loss of motility and viability. However, various studies have demonstrated that physiological amounts of ROS play important roles in the processes of spermatozoa maturation, capacitation, hyperactivation and acrosome reaction. It is therefore crucial to define and understand the delicate oxidative balance in male reproductive cells and tissues for a better understanding of both positive as well as negative impact of ROS production on the fertilizing ability. This review will discuss the specific physiological roles, mechanisms of action and effects that ROS have on the acquisition of structural integrity and physiological activity of spermatozoa.

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Human sperm chemotaxis depends on critical levels of reactive oxygen species

Cited by 23 publications

References 18 publications

Endogenous reactive oxygen species content and modulation of tyrosine phosphorylation during sperm capacitation

Endogenous reactive oxygen species content and modulation of tyrosine phosphorylation during sperm capacitation

Supplementation of IVF medium with melatonin: effect on sperm functionality andin vitroproduced bovine embryos

Contemporary evidence on the physiological role of reactive oxygen species in human sperm function

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