Human Sperm-Oocyte Fusion

Sathananthan, A. Henry; Ng, Soon-Chye; Trounson, Alan; Ratnam, S. S.; Bongso, T. A.

doi:10.1007/978-3-642-83965-8_24

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…The faint fluorescence observed after DNA staining indicates less condensed DNA of the egg nucleus as has been previously observed in maize (Kranz et al, 1991), perennial ryegrass (Van der Maas et al, 1993) and wheat (Kovacs et al, 1994). In humans it has been demonstrated (Sathananathan et al, 1990) that the nuclear chromatin undergoes decondensation. We are currently looking at the karyoskeletal proteins associated with the process of decondensation of sperm nuclear chromatin in the fusion products.…”

Section: Changes In Cellulosesupporting

confidence: 57%

“…Our study provides the first visual comparisons of intermingling of sperm chromatin with those of egg nuclei in that a major portion of the nuclear chromatin of the sperm seems to intermingle not at the site of nuclear membrane fusion but in deeper areas of the egg nuclei below the nuclear membrane. In humans it has been demonstrated (Sathananathan et al, 1990) that the nuclear chromatin undergoes decondensation. The faint fluorescence observed after DNA staining indicates less condensed DNA of the egg nucleus as has been previously observed in maize (Kranz et al, 1991), perennial ryegrass (Van der Maas et al, 1993) and wheat (Kovacs et al, 1994).…”

Section: Nuclear Chromatin Of the Sperm Before And After Karyogamymentioning

confidence: 99%

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Characterisation of isolated egg cells,in vitrofusion products and zygotes ofZea maysL. using the technique of image analysis and confocal laser scanning microscopy

Tirlapur

Kranz²,

Cresti³

1995

Zygote

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Changes in membrane Ca 2+ , calcium receptor protein calmodulin, endoplasmic reticulum (ER), mitochondria and cellulose in unfixed, living, isolated egg cells and fusion products of pairs of one egg and one sperm cell of Zea mays L. have been investigated using chlorotetracycline, fluphenazine, immunocytochemical techniques, 3,3'-dihexyloxa-carbocyanine iodide (DiOC6 (3)) and calcofluor white in conjunction with computer-controlled video image analysis. In addition, confocal laser scanning microscopy has been used in conjunction with ethidium bromide to detect the nature and location of the sperm cell nuclear chromatin before and after karyogamy. Digitised video images of chlorotetracycline (CTC) fluorescence reveal that egg cells contain high levels of membrane Ca 2 + in organelles present around the nucleus while the cytosolic signal is relatively low. Intense CTC fluorescence is invariably present just below the plasma membrane of egg cells and a certain degree of regionalised distribution of Ca 2+ in cytoplasm is also discernible. Similarly, the fluphenazine (FPZ)-detectable calmodulin (CaM) and that localised immunocytochemically using monoclonal anti-CaM antibodies reveal high levels of CaM in the vicinity of the nucleus in egg cells. Only a few ER profiles and mitochondria could be visualised in the egg cell and no calcofluor fluorescence could be detected. Following in vitro fertilisation of single isolated eggs substantial changes in the Ca 2 + levels occur which include an increase in the membrane Ca 2+ of the fusion product, particularly in the cytosol and around the nucleus. Unlike in the eggs the fine CTC fluorescence signal below the plasma membrane is not detectable in the fusion products. Compared with isolated egg cell protoplasts an increase in the CaM level in the cytoplasm was observed in the fusion products. There is a slight increase in the fluorescence associated with ER profiles in the fusion products compared with egg cell protoplasts. A distinct calcofluor fluorescence around the fusion product is visible after 16 h in culture. The sperm cell chromatin in the fusion product is highly condensed, unlike that of the egg cell, and confocally imaged serial optical sections of the in vitro fusion product reveal the occurrence of karyogamy 35 min following gamete fusion. First visual evidence for intermingling of sperm nuclear chromatin in the zygotic nuclei is also provided.

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Section: Changes In Cellulosesupporting

confidence: 57%

Section: Nuclear Chromatin Of the Sperm Before And After Karyogamymentioning

confidence: 99%

Characterisation of isolated egg cells,in vitrofusion products and zygotes ofZea maysL. using the technique of image analysis and confocal laser scanning microscopy

Tirlapur

Kranz²,

Cresti³

1995

Zygote

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Silver staining of the postacrosomal sheath during human spermiogenesis

Sousa

Azevedo

1992

Anat Embryol

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Applying the silver staining technique, it could be shown that in the early phase of spermiogenesis a layer of argyrophilic material accumulated at the base of the acrosomal vesicle and at the outer side of the nuclear envelope opposite that region, and, later, at the inner side of the plasma membrane near the base of the acrosomal vesicle. During further development of the postacrosomal region of the spermatozoon head, the argyrophilic material associated with the plasmalemma grew caudally to form the postacrosomal dense lamina, while the argyrophilic material associated with the nuclear envelope, staying the same size, shifted to the caudal end of the postacrosomal dense lamina to form the post-nuclear band.

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First Stages of Development

2019

In-Vitro Fertilization

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Human Sperm-Oocyte Fusion

Cited by 4 publications

References 30 publications

Characterisation of isolated egg cells,in vitrofusion products and zygotes ofZea maysL. using the technique of image analysis and confocal laser scanning microscopy

Characterisation of isolated egg cells,in vitrofusion products and zygotes ofZea maysL. using the technique of image analysis and confocal laser scanning microscopy

Silver staining of the postacrosomal sheath during human spermiogenesis

First Stages of Development

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