Human Spermatozoal Nuclear Maturity in Normozoospermia and Asthenozoospermia

Colleu, D.; Lescoat, D.; Boujard, Daniel; Lannou, D. Le

doi:10.3109/01485018808986737

Cited by 29 publications

(15 citation statements)

References 16 publications

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“…The positive correlation between chromatin condensation (assessed by gram stain) and sperm morphology shows that spermatozoa shape and size is affected by their nuclear status (normal nuclear status results in better sperm morphology). This is in agreement with previous studies, which prove that the percentage of nuclei with normal chromatin condensation is much higher in morphologically normal spermatozoa than in spermatozoa with abnormal morphology [7,19] . The strong positive correlation between aniline blue and Gram staining ( table 3 ) should be noted, since it shows the connection between human spermatozoa chromatin condensation and nuclear protein composition.…”

Section: Discussionsupporting

confidence: 82%

Evaluation of Gram Stain as an Alternative in the Assessment of Human Spermatozoa Quality

2006

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Introduction: During spermiogenesis, protaminosis and sperm chromatin condensation are important prerequisites for the preservation of DNA integrity in spermatozoa. The aim of this study is to assess Gram stain as an alternative technique for the evaluation of human sperm chromatin condensation status. Patients and Methods: Aniline blue and Gram staining were applied to semen samples from 34 donors in order to determine the relationship between sperm chromatin condensation and infertility. In addition, the possible correlation between morphology and vitality (eosin-Y staining) of spermatozoa compared with their nuclear status (aniline blue and Gram staining) was studied. Results: Chromatin condensation and sperm vitality were significantly higher in fertile men compared to the subfertile. A significant correlation was found between chromatin condensation and (a) sperm vitality (p < 0.01), and (b) nuclear protein status (p < 0.01). Conclusions: Gram staining may be used as a routine method in assisted reproduction laboratories and could assist in the evaluation of sperm quality as well as in the selection of the appropriate fertilization technique.

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Section: Discussionsupporting

confidence: 82%

Evaluation of Gram Stain as an Alternative in the Assessment of Human Spermatozoa Quality

2006

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“…Aniline blue selectively stains lysine-rich histones (Terquem and Dadoune, 1983) and has been used as a clinical assessment of sperm chromatin condensation and associated infertility (Auger et al, 1990). High percentages of aniline blue-stained spermatozoa were found in patients affected by severe teratozoospermia (Franken et al, 1999), asthenozoospermia (Colleu et al, 1988), varicocele, idiopathic infertility, and with a history of unilateral cryptorchidism (Foresta et al, 1992). Furthermore, higher levels of aniline blue staining were found in subfertile men following cryopreservation than in fertile controls (Hammadeh et al, 1999b).…”

Section: Assessment Of Sperm Chromatin Structure In Fertility Evaluatmentioning

confidence: 99%

Sperm Chromatin Structure Assay: Its Clinical Use for Detecting Sperm DNA Fragmentation in Male Infertility and Comparisons With Other Techniques

Evenson

Larson

Jost

2002

Journal of Andrology

908

768

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The Sperm Chromatin Structure Assay (SCSA) was first described in the December 5, 1980 issue of Science (Evenson et al, 1980a). The data described in that article showed a significant difference between proven fertile and subfertility or infertility in men and bulls and the susceptibility to denaturation of their sperm nuclear DNA. A subsequent study (Evenson et al, 1984) described SCSA data obtained from men with testicular cancer. The data were heterogeneous, and begged the question, what was SCSA measuring? Great effort has been spent over the past 15 years on animal model systems, mostly related to dose-response toxicology experiments and large animal fertility trials. The results from these studies showed that SCSA was highly dose-responsive to toxicants, highly repeatable, and provided meaningful biological information on sperm nuclear DNA defects. The extensive data from nonhumans (Evenson et al, 1985(Evenson et al, , 1989a(Evenson et al, ,b, 1993a(Evenson et al, ,b,c, 1994Ballachey et al, 1987Ballachey et al, , 1988Evenson and Jost, 1993; Sailer et al, 1995a,b;Evenson, 1999a), and human toxicology and fertility studies (Evenson et al, 1978(Evenson et al, , 1980a(Evenson et al, ,b, 1984(Evenson et al, , 1991Evenson and Melamed, 1983;Evenson, 1999b;Larson et al, 1999Larson et al, , 2000Larson et al, , 2001 provide This work was supported in part by grant R827019 from the US Environmental Protection Agency, grants EPS-9720642 and OSR-9452894 from the National Science Foundation, and by SCSA Diagnostics, Inc. This is South Dakota Agricultural Experiment Station publication 3273 of the journal series.Correspondence to: Donald P. Evenson, Olson Biochemistry Laboratories, ASC 136, South Dakota State University, Brookings, SD 57006 (e-mail: donald evenson@sdstate.edu).Received for publication September 20, 2001; accepted for publication September 20, 2001. compelling evidence that SCSA will be useful in clinical human semen analysis (Figure 1).After reviewing current infertility center sites on the World Wide Web and approximately a dozen lay-oriented books on infertility and assisted reproductive technology (ART), it was amazing that not a single reference contained any information about the negative influence of fragmented sperm nuclear DNA on successful pregnancy outcome. Furthermore, few physicians are aware of scientific literature on this subject. Currently, if a spermatozoon has some motility as seen under a light microscope, and reasonable morphology, then many ART clinics assume that the sperm DNA must be fine. However, a number of cases have been documented in which couples have had multiple failed attempts at intracytoplasmic sperm injection (ICSI), even using donor eggs, and we found that the fragmentation of sperm DNA was above our threshold for less than 1% probability of achieving a successful pregnancy. Spermatozoa with defective DNA can fertilize an oocyte, produce high-quality early stage embryos, and then, in relationship to the extent of DNA damage, fail in producing a successful term ...

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“…Another indirect approach to investigate the status of the sperm chromatin has been the use of aniline blue staining to detect the presence of histones and, therefore, indirectly infer the presence of lower amounts of protamines in the sperm nucleus [155,182]. An increase in the percentage of aniline blue cells was found in asthenozoospermic samples as compared to normozoospermic ones [182].…”

Section: Protamine Anomalies In the Sperm Cells Of Infertile Patientsmentioning

confidence: 92%

Sperm Nucleoproteins

Oliva¹,

Castillo²

2011

Sperm Chromatin

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Human Spermatozoal Nuclear Maturity in Normozoospermia and Asthenozoospermia

Cited by 29 publications

References 16 publications

Evaluation of Gram Stain as an Alternative in the Assessment of Human Spermatozoa Quality

Evaluation of Gram Stain as an Alternative in the Assessment of Human Spermatozoa Quality

Sperm Chromatin Structure Assay: Its Clinical Use for Detecting Sperm DNA Fragmentation in Male Infertility and Comparisons With Other Techniques

Sperm Nucleoproteins

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