Human Synaptonemal Complex Protein 1 (SCP1): Isolation and Characterization of the cDNA and Chromosomal Localization of the Gene

Meuwissen, Roger; Meerts, I.; Hoovers, J. M. N.; Leschot, N. J.; Heyting, C.

doi:10.1006/geno.1996.4373

Cited by 46 publications

(26 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When screening this library with a serum from a renal cell carcinoma patient, we identified HOM-TES-14, which turned out to be encoded by the gene for SCP-1. Although library screening with cDNA probes had failed to identify additional SCP-1-related genes (22,23), our Southern blot analysis suggests that SCP-1 belongs to a family of related genes. We confirmed previous reports (24) that human SCP-1 is not expressed in normal tissues except for testis.…”

Section: Discussionmentioning

confidence: 77%

Identification of a meiosis-specific protein as a member of the class of cancer/testis antigens

Türeci

Şahin

Zwick

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

238

166

View full text Add to dashboard Cite

Little is known about the function of human cancer͞testis antigens (CTAs), such as MAGE, BAGE, GAGE, HOM-MEL-40, and NY-ESO-1, the expression of which is restricted to human malignancies and testis. When screening a cDNA expression library enriched for testis-specific representative long transcripts for reactivity with high-titered IgG antibodies from the serum of a patient with renal cell carcinoma, one repeatedly detected antigen, designated HOM-TES-14, turned out to be encoded by the synaptonemal complex protein 1 (SCP-1) gene. SCP-1 is known to be selectively expressed during the meiotic prophase of spermatocytes and is involved in the pairing of homologous chromosomes, an essential step for the generation of haploid cells in meiosis I. Investigation of a broad spectrum of normal and malignant tissues revealed expression of SCP-1 transcripts and antigen selectively in a variety of neoplastic tissues and tumor cell lines. Immunof luorescence microscopy analysis with specific antiserum showed a cell cycle phaseindependent nuclear expression of SCP-1 protein in cancer cells. SCP-1 differs from other members of the class of CTA by its localization on chromosome 1 and its frequent expression in malignant gliomas, breast, renal cell, and ovarian cancer. The aberrant expression of SCP-1 in tumors might contribute to their genomic instability and suggests that the functional role of other CTA might also relate to meiosis.The identification of genes that are selectively expressed in cancer and code for proteins inducing a specific immune response in the tumor-bearing host is the prerequisite for specific immunotherapeutic approaches to cancer. By using cytotoxic T lymphocyte-and antibody-based approaches, several human tumor antigens have been defined that might be suitable targets for specific cancer vaccination (1, 2). The difficulties in establishing cytotoxic T lymphocyte clones with specificity to human neoplasms are the major reason whywith the exception of RAGE (renal cell cancer-associated antigen) (3)-all T cell-defined human tumor antigens have been originally described in malignant melanoma. Immunotherapeutic approaches to human neoplasms other than melanoma are restricted to the nonmelanoma cases that express the melanoma-defined antigens. To overcome this limitation, we recently established SEREX (serological analysis of antigens by recombinant expression cloning) that allows for the unbiased search of tumor antigens that elicit IgG antibody responses in the autologous tumor patients (4). With this approach, we have identified multiple tumor antigens in different human neoplasms. Among others, we detected MAGE-1 (melanoma-associated antigen) and MAGE4a transcripts, demonstrating that at least some of the serologically identified antigens are also targets for cytotoxic T cells.

show abstract

Section: Discussionmentioning

confidence: 77%

Identification of a meiosis-specific protein as a member of the class of cancer/testis antigens

Türeci

Şahin

Zwick

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

238

166

View full text Add to dashboard Cite

show abstract

“…The CO/NCO decision, the ZMM proteins in mammals Four mammalian homologs of the yeast ZMM proteins have been identified: Sycp1 (Sage et al 1995, Meuwissen et al 1997, de Vries et al 2005, Msh4 (Paquis-Flucklinger et al 1997, Kneitz et al 2000, Msh5 (Bocher et al 1999, Her et al 1999, Edelmann et al 1999 and Mer3 (also called HFM1, Entrez IDs: 330149 and 164045 for mouse and human, respectively). The Mer3 gene and protein have not been analyzed.…”

Section: Properties Of Nco Productsmentioning

confidence: 99%

Regulating double-stranded DNA break repair towards crossover or non-crossover during mammalian meiosis

2007

View full text Add to dashboard Cite

During meiosis the programmed induction of DNA double-stranded breaks (DSB) leads to crossover (CO) and non-crossover products (NCO). One key role of CO is to connect homologs before metaphase I and thus to ensure the proper reductional segregation. This role implies an accurate regulation of CO frequency with the establishment of at least one CO per chromosome arm. Current major challenges are to understand how CO and NCO formation are regulated and what is the role of NCO. We present here the current knowledge about CO and NCO and their regulation in mammals. CO density varies widely along chromosomes and their distribution is not random as they are subject to positive interference. As documented in the mouse and human, a significant excess of DSB are generated relative to the number of CO. In fact, evidence has been obtained for the formation of NCO products, for regulation of the choice of DSB repair towards CO or NCO and for a CO specific pathway. We discuss the roles of Msh4, Msh5 and Sycp1 which affect DSB repair and probably not only the CO pathway. We suggest that, in mammals, the regulation of NCO differs from that described in Saccharomyces cerevisiae.

show abstract

“…TF proteins have therefore been identified independently in only a limited number of species, namely, mammals (SYCP1) (Dobson et al 1994;Meuwissen et al 1992;Meuwissen et al 1997;Sage et al 1999), budding yeast (Zip1) (Sym et al 1993), Drosophila [C(3)G] (Page and Hawley 2001), and Caenorhabditis (Syp-1 and Syp-2) (Colaiácovo et al 2003;MacQueen et al 2002). Despite their lack of amino acid sequence conservation, SYCP1 (Meuwissen et al 1992), Zip1 (Sym et al 1993), and C(3)G (Page and Hawley 2001) have similar structures (discussed by Bogdanov et al 2002;Higgins et al 2005): they are long, coiled-coil proteins with globular domains at both ends.…”

Section: Transverse Filamentsmentioning

confidence: 99%

The diverse roles of transverse filaments of synaptonemal complexes in meiosis

Boer

Heyting

2006

Chromosoma

View full text Add to dashboard Cite

In most eukaryotes, homologous chromosomes (homologs) are closely apposed during the prophase of the first meiotic division by a ladderlike proteinaceous structure, the synaptonemal complex (SC) [Fawcett, J Biophys Biochem Cytol 2:403-406, 1956; Moses, J Biophys Biochem Cytol 2: [215][216][217][218] 1956]. SCs consist of two proteinaceous axes, which each support the two sister chromatids of one homolog, and numerous transverse filaments (TFs), which connect the two axes. Organisms that assemble SCs perform meiotic recombination in the context of these structures. Although much information has accumulated about the composition of SCs and the pathways of meiotic crossing over, several questions remain about the role of SCs in meiosis, in particular, about the role of the TFs. In this review, we focus on possible role(s) of TFs. The interest in TF functions received new impulses from the recent characterization of TF-deficient mutants in a number of species. Intriguingly, the phenotypes of these mutants are very different, and a variety of TF functions appear to be hidden behind a façade of morphological conservation. However, in all TFdeficient mutants a specific class of crossovers that display interference is affected. TFs appear to create suitable preconditions for the formation of these crossovers in most species, but are most likely not directly involved in the interference process itself. Furthermore, TFs are important for full-length homolog alignment. (Fig. 1). Assembly and composition of synaptonemal complexesCohesins, which are proteins that mediate cohesion between sister chromatids (reviewed by Nasmyth 2005), are important for AE assembly (Klein et al. 1999;Pelttari et al. 2001). During S-phase, both in the mitotic cycle and in premeiotic S-phase, cohesins are installed in such a way that they hold the newly synthesized sister chromatids together. In the mitotic cycle, all sister chromatid cohesion is lost at the metaphase-to-anaphase transition, so that sister chromatids can disjoin. In meiosis, two chromosome segregations follow a single round of DNA-replication, and this is possible because cohesion is released in two steps (reviewed by Nasmyth 2001Nasmyth , 2005.Cohesins not only provide sister chromatid cohesion, but also participate in homologous recombination, both in the mitotic cycle and in meiosis. In mitosis, they enhance sister chromatid-based recombinational repair. In meiosis, their role is modified in such a way that homologous recombination occurs preferentially between chromatids of homologs, in most species, mainly or exclusively by a TF-dependent pathway of crossing over (reviewed by

show abstract

Human Synaptonemal Complex Protein 1 (SCP1): Isolation and Characterization of the cDNA and Chromosomal Localization of the Gene

Cited by 46 publications

References 15 publications

Identification of a meiosis-specific protein as a member of the class of cancer/testis antigens

Identification of a meiosis-specific protein as a member of the class of cancer/testis antigens

Regulating double-stranded DNA break repair towards crossover or non-crossover during mammalian meiosis

The diverse roles of transverse filaments of synaptonemal complexes in meiosis

Contact Info

Product

Resources

About