Human T- and B-Cell Responses toSchistosoma mansoniRecombinant Glyceraldehyde 3-Phosphate Dehydrogenase Correlate with Resistance to Reinfection withS. mansoniorSchistosoma haematobiumafter Chemotherapy

Abstract

Recently we reported that human T-and B-cell recognition of a 42-kDa protein (p42) in soluble extracts of adult Schistosoma mansoni worms correlates with resistance to reinfection with S. mansoni or S. haematobium. Amino acid microsequencing of p42 revealed that it consists predominantly of schistosome glyceraldehyde 3-phosphate dehydrogenase (SG3PDH). We have expressed SG3PDH in Escherichia coli and purified the recombinant protein in a soluble and enzymatically active form. Recombinant SG3PDH (rSG3PDH) reacted with human monospecific antibodies to p42. Lymphoproliferation and production of interleukin-4 and gamma interferon (IFN-␥) after in vitro stimulation with rSG3PDH and serum isotype responses to rSG3PDH were examined in individuals with extremes of resistance and susceptibility to reinfection after treatment of previous S. mansoni or S. haematobium infection. Lymphoproliferation and IFN-␥ production in response to rSG3PDH and the presence of serum immunoglobulin G1 (IgG1), IgG3, and IgA antibodies to rSG3PDH generally characterized individuals who are resistant to reinfection after chemotherapy. The data indicate that T-and B-cell immune reactivity to rSG3PDH correlates with resistance to reinfection, confirming previous studies identifying SG3PDH as a target of protective immunity in humans, and suggest that SG3PDH should be investigated as a possible vaccine for human schistosomiasis.

Efficacy and Safety of Arachidonic Acid for Treatment of Schistosoma mansoni-Infected Children in Menoufiya, Egypt

“…Heparinized whole blood was diluted 1:4 in Roswell Park Memorial Institute (RPMI) -1640 medium supplemented with 200 units/mL penicillin, 200 μg/mL streptomycin, 50 ng/mL amphotericin, and 20 μg/mL polymyxin B (Sigma-Aldrich) as an inhibitor of any residual lipopolysaccharide contamination of antigen; 200 μL diluted blood was incubated in duplicate with 50 μL medium containing 0 or 40 μg/mL recombinant S. mansoni glyceraldehyde 3-phosphate dehydrogenase (rSG3PDH) prepared as described previously. 35 Whole-blood cultures were incubated for 72 hours at 37 C and 3% CO 2 and then centrifuged at 400 + g for 10 minutes. The cell-free supernatants were transferred into wells of sterile plates and stored at −76 C until assayed by capture ELISA for levels of released IL-4, IL-17, and IFN-γ (BioLegend) following the manufacturer's instructions.…”

Impact of interleukin-1 and interleukin-6 in murine primary schistosomiasis

“…It was recently shown that a 42 kDa soluble adult worm antigen band, identified as S. mansoni glyceraldehyde 3-phosphate dehydrogenase (SG3PDH), is a target of cellular and humoral immune responses in subjects resistant to infection with schistosomes [7,8]. Additionally, several schistosomular and adult worm antigens were detected by sera from mice immunized with radiation-attenuated cercariae (RA), among which Sm23, glutathione S-transferase, triose phosphate isomerase, Sm32, heat shock protein, calreticulin, and paramyosin are the best studied [9,10].…”

Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase is a lung-stage schistosomula surface membrane antigen

“…The coding sequence for SG3PDH was obtained and expressed, and the recombinant protein (rSG3PDH) was purified by metal affinity chromatography (HiTrap TM Affinity Column, Pharmacia) as detailed previously (El Ridi et al 2001b). Antigen aliquots were kept until use at -76°C and thawed only once.…”