Human T-cell cultures from virus-sensitized donors can mediate virus-specific and HLA-restricted cell lysis

Sethi, K. K.; Stroehmann, I.; Brandis, H.

doi:10.1038/286718a0

Cited by 46 publications

(21 citation statements)

References 24 publications

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“…Similar conclusions have been reached from in vitro studies of virus-specific cytotoxic reactions with human lymphocytes (Sethi et al, 1980). It has also been shown that mixed vesicles containing virus structural proteins and H-2 antigens can trigger secondary antiviral effector cells in vitro (Ciavarra et aL, 1980).…”

Section: Discussionsupporting

confidence: 65%

In vitro Stimulation of Rabbit T Lymphocytes by Cells Expressing Herpes Simplex Antigens

Kapoor¹,

Ling²,

Nash³

et al. 1982

Journal of General Virology

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Section: Discussionsupporting

confidence: 65%

In vitro Stimulation of Rabbit T Lymphocytes by Cells Expressing Herpes Simplex Antigens

Kapoor¹,

Ling²,

Nash³

et al. 1982

Journal of General Virology

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“…It has been reported that human HSV-specific CTL and NK cells can lyse virusinfected target cells in a short term SlCr release assay (Sethi et al, 1980;Yasukawa & Zarling, 1983). These findings suggest that the low virus yields obtained in our experiments may be attributable to the CTL-and NK cell-mediated lysis of infected target cells before virus assembly.…”

Section: It Is Well Known That Cytotoxic T Cells (Ctl) and Natural Kimentioning

confidence: 99%

Inhibition of Herpes Simplex Virus Replication in vitro by Human Cytotoxic T Cell Clones and Natural Killer Cell Clones

Yasukawa

Kobayashi

1985

Journal of General Virology

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2225 SUMMARYThe abilities of human cytotoxic T cell (CTL) clones and natural killer (NK) cell clones to inhibit the replication of herpes simplex virus (HSV) in vitro were shown. The specificities of clones inhibiting HSV replication were the same as those of cytotoxicity in HSV type specificity and HLA restriction, i.e. HSV type 1 (HSV-I) and HSV type 2 (HSV-2) common CTL clones inhibited the replication of both HSV-I and HSV-2 in autologous cells, but not in allogeneic cells. HSV-l-specific CTL clones inhibited the replication of HSV-1 in autologous cells; however, the replication of HSV-2 was not inhibited. NK cell clones inhibited the replication of HSV-1 and HSV-2 in autologous and allogeneic cells. This is the first demonstration that human virus-specific CTL clones and NK cell clones directly limit virus replication in vitro.

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“…In the murine system, adoptive immunotherapy and selective immunodepletion experiments have indicated that CD8+ CTL protect against HSV challenge ( 1,2). Human HSV-specific CTL responses detected in bulk culture arise from both CD4+ (3)(4)(5)(6)(7)(8) and CD8 + (5,6,9) cytolytic effector cells. Recently, by using activated PBMC infected with HSV as in vitro stimulator cells, we reported the isolation of HSV-specific CD8+ CTL clones capable of lysing HSV-infected autologous or HLA class I-matched Epstein-Barr virus-transformed B cell line (LCL) (10).…”

Section: Introductionmentioning

confidence: 99%

Herpes simplex virus infection of human fibroblasts and keratinocytes inhibits recognition by cloned CD8+ cytotoxic T lymphocytes.

Koelle¹,

Tigges²,

Burke³

et al. 1993

J. Clin. Invest.

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CD8' cytotoxic T lymphocytes (CTL) clones with specificity for herpes simplex virus (HSV) were derived from two donors with genital HSV-2 infection. These CIL clones specifically lysed HSV-infected autologous B lymphoblastoid cells, but not HSV-infected fibroblasts. Exogenous peptide loading sensitized both cell types to lysis by an HSV-specific ClL clone of known specificity. HSV infection rendered fibroblasts refractory to peptide sensitization. HSV infection also rendered fibroblasts and keratinocytes insensitive to lysis by allospecific CD8 + CI'L clones. Lysis of B lymphoblastoid cells in this system was only slightly reduced by HSV infection. Reduction of fibroblast allospecific lysis was dose and time dependent and was blocked by acyclovir, indicating the involvement of a late HSV gene product. HSV caused a reduction of fibroblast cell surface HLA class I antigen, at least in part due to reduction of synthesis of heavy chain-f32 microglobulin heterodimers. These results suggest that HSV-induced blockade ofantigen presentation by cutaneous cells to CD8+ CTL may be a mechanism by which HSV limits or evades the immune response of the host. (J. Clin. Invest. 1993. 91:961-968.)

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Human T-cell cultures from virus-sensitized donors can mediate virus-specific and HLA-restricted cell lysis

Cited by 46 publications

References 24 publications

In vitro Stimulation of Rabbit T Lymphocytes by Cells Expressing Herpes Simplex Antigens

In vitro Stimulation of Rabbit T Lymphocytes by Cells Expressing Herpes Simplex Antigens

Inhibition of Herpes Simplex Virus Replication in vitro by Human Cytotoxic T Cell Clones and Natural Killer Cell Clones

Herpes simplex virus infection of human fibroblasts and keratinocytes inhibits recognition by cloned CD8+ cytotoxic T lymphocytes.

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