Human T-cell leukemia virus-associated membrane antigens: identity of the major antigens recognized after virus infection.

Lee, T H; Coligan, J E; Homma, Takao; McLane, Mary Fran; Tachibana, Nobuyoshi; Essex, Max

doi:10.1073/pnas.81.12.3856

Cited by 103 publications

(53 citation statements)

References 33 publications

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“…1). Both HTLV-1 and HTLV-2 express the structural and enzymatic proteins Gag, Pol and Pro from the unspliced full length mRNA (Lee et al, 1984;Nam et al, 1988). This unspliced mRNA also serves as genomic RNA to be packaged into progeny virions.…”

Section: Introductionmentioning

confidence: 99%

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR

Green

2007

Journal of Virological Methods

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SummaryHTLV-1 and HTLV-2 are highly related delta-retroviruses that infect and transform T-lymphocytes, but have distinct pathogenic properties. HTLV replication and survival requires the expression of multiple gene products from an unspliced and a series of highly related alternatively spliced mRNA species. To date, the comparative levels of all known HTLV-1 and HTLV-2 viral mRNAs in different transformed cell lines and at different stages of virus infection have not been assessed. In this study, we compiled a series of oligonucleotide primer pairs and probes to quantify both HTLV-1 and HTLV-2 mRNA species using real-time RT-PCR. The optimized reaction for detection of each mRNA had amplification efficiency greater than 90% with a linear range spanning 25 to 2.5 × 10 7 copies. The R 2 s of all standard curves were greater than 0.97. Quantitation of HTLV mRNAs between different cell lines showed variability (gag/pol ≥ tax/rex > env ≥ accessory proteins), but the overall levels of each mRNA relative to each other within a cell line were similar. These results provide a method to quantify all specific mRNAs from both HTLV-1 and HTLV-2, which can be used to evaluate further viral gene expression and correlate transcript levels to key stages of the virus life cycle and ultimately, pathogenesis.

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Section: Introductionmentioning

confidence: 99%

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR

Green

2007

Journal of Virological Methods

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“…We previously identified a glycoprotein, gp6l, expressed on the cytoplasmic membrane of a HTLV-I-infected prototype tumor cell line, HUT 102 (clone B2) (21)(22)(23). This antigen is the most immunogenic species in HTLV-exposed humans (21).…”

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confidence: 99%

“…This antigen is the most immunogenic species in HTLV-exposed humans (21). It can be precipitated by serum samples from ATLL patients (21), healthy carriers living in endemic areas (21), and with most acquired immune deficiency syndrome (AIDS) and hemophiliac patient antisera that are positive for antibody to HTLV-associated cell membrane antigen (HTLV-MA) as measured by membrane immunofluorescence (21)(22)(23).…”

mentioning

confidence: 99%

“…It can be precipitated by serum samples from ATLL patients (21), healthy carriers living in endemic areas (21), and with most acquired immune deficiency syndrome (AIDS) and hemophiliac patient antisera that are positive for antibody to HTLV-associated cell membrane antigen (HTLV-MA) as measured by membrane immunofluorescence (21)(22)(23). Using radiolabel sequencing analysis, we have concluded that gp61 is an env gene-encoded product of HTLV-ICR (21).…”

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confidence: 99%

“…The HTLV-ICR-infected Hut 102 clone B2 (Hut 102) has been described (1,21,24). Uninfected cell lines 8402, Jurkat, and NC37 have also been described (21). C3-44/MO is a HTLV-lIMO-infected cell line (25).…”

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Serological cross-reactivity between envelope gene products of type I and type II human T-cell leukemia virus.

Lee¹,

Coligan²,

McLane³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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People exposed to type I human T-cell leukemia virus (HTLV-I) develop antibodies to an antigen at the surface of virus-infected cells, designated human T-cell leukemia virus membrane antigen (HTLV-MA). In an earlier study, we demonstrated that the major component of HTLV-MA is gp6l, a glycoprotein encoded by the HTLV env gene. In the current study, we found that human antibodies that react with HTLV-MA on cells infected with HTLV-I react equally well with HTLV-MA on C3-44/MO, a target cell infected with type II HTLV. A glycoprotein with an approximate size of 67 kDa, gp67, was identified in C3-44/MO using immunoprecipitation and NaDodSO4/PAGE analysis. The positions of serine and cysteine residues were determined in the amino terminus of gp67 by radiolabel sequencing analysis. Comparison with the amino acid sequence deduced from the primary nucleotide sequence of HTLV-IIMo virus reveals that gp67 is also encoded, at least in part, by the env gene. The gp67 of HTLV-IIMo, like the env gene product of HTLV-ICR, gp6l, is recognized both by antibodies from a HTLV-IIMO-infected patient with a variant form of hairy cell leukemia, and by antibodies from patients with HTLV-I-associated adult T-cell leukemia/lymphoma. These results indicate that, despite the divergence between HTLV-I and HTLV-II, the major env gene products of the two types of HTLV are conserved to the degree that they are serologically cross-reactive.

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Osthole Increases Glycosylation of Hepatitis B Surface Antigen and Suppresses the Secretion of Hepatitis B Virus In Vitro

Huang¹,

Chen²,

Huang³

et al. 1996

Hepatology

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Chinese medicinal herbs have been used for centuries for During screening of Chinese herbal medicines for the treatment of liver diseases. 8 An extract from Phyllanthus activities against hepatitis B virus (HBV), a known pure amarus, one of the commonly used Chinese folk herbs, has compound, osthole, was found to inhibit the secretion of been reported to have a specific effect against HBV. [9][10][11] There-HBV surface antigens in vitro. The secretion of hepatitis fore we initiated screening of Chinese herbs for anti-HBV B surface antigen (HBsAg) in culture medium of MS-G2 activity using an HCC cell line transfected with HBV DNA. hepatitis, affecting at least 300 million people worldwide. HuH-7 cells was performed as described previously. (1.72 kg) was obtained. The residue was partitioned between water and chloroform and the chloroform extract (242 g) was chromatographed on a silica gel column and eluted with gradients of n-hex- Osthole in varying concentrations was dissolved in 10 mg/mL di- Received September 19, 1995; accepted May 10, 1996. methyl sulfoxide (DMSO) and added to MS-G2 or HuH-7 cells 4 days fresh medium containing osthole (in DMSO) or DMSO only. MATERIALS AND METHODS Immunoprecipitation of culture medium showed that

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Human T-cell leukemia virus-associated membrane antigens: identity of the major antigens recognized after virus infection.

Cited by 103 publications

References 33 publications

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR

Serological cross-reactivity between envelope gene products of type I and type II human T-cell leukemia virus.

Osthole Increases Glycosylation of Hepatitis B Surface Antigen and Suppresses the Secretion of Hepatitis B Virus In Vitro

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