Human T-cell lymphotropic virus (HTLV)-related endogenous sequence, HRES-1, encodes a 28-kDa protein: a possible autoantigen for HTLV-I gag-reactive autoantibodies.

Bánki, Katalin; Maceda, J; Hurley, Edward L.; Ablonczy, E; Mattson, David H.; L, Szegedy; Hung, Chien-Jen; Perl, András

doi:10.1073/pnas.89.5.1939

Cited by 142 publications

(106 citation statements)

References 35 publications

(48 reference statements)

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“…The human endogenous retroviral sequence termed HRES-1 could be overexpressed in salivary gland epithelium of patients with SS (31). Anti-HRES-l/p28 antibodies, which can cross-react with HTLV-I gag p24 polypeptide, were found in 10% of SS patients (32). Recently, another gag antigen of HTLV-I, p19, was detected in LSG epithelial cells from -30% of SS patients, and not from control subjects (33).…”

Section: Discussionmentioning

confidence: 99%

Detection of human t lymphotropic virus type i tax gene in salivary gland epithelium from two patients with sjögren's syndrome

Mariette

Agbalika

Daniel

et al. 1993

Arthritis & Rheumatism

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Objective. To investigate whether human T lymphotropic virus type I (HTLV‐I) could be involved in the pathogenesis of Sjögren's syndrome (SS). Methods. Labial salivary gland (LSG) biopsy specimens from 9 patients with SS (4 with primary SS and 5 with SS secondary to rheumatoid arthritis) and 9 controls were studied for the presence of the tax gene of HTLV‐I using in situ hybridization, and for the presence of tax, gag, pol, and env genes of HTLV‐I using the polymerase chain reaction (PCR). Testing for antibodies to HTLV‐I and examination of lymphocytes on blood smears were performed to determine whether systemic viral infection was present. Results. Using in situ hybridization and PCR, we detected the tax gene, but not the gag, pol, or env genes, of HTLV‐I in LSG sections from 2 of 9 patients with SS and from none of the control subjects. Tax DNA was present mostly in nuclei of epithelial cells, but also in some lymphoid cells. Serum of the 2 affected patients did not contain antibodies to HTLV‐I. In 1 patient, examination of blood smears revealed rare convoluted lymphocytes, sometimes with the appearance of “flower cells,” as observed in the blood of HTLV‐I–infected patients. Conclusion. None of the known endogenous retroviral sequences is homologous to the tax gene. Thus, we suggest that HTLV‐I (or another related retrovirus) can infect salivary epithelium. Transactivation properties of the tax protein could be implicated in the pathogenesis of SS. Alternatively, viral infection could cause de novo expression of HLA–DR antigens and favor the presentation of antigens by epithelial cells, leading, in some genetically predetermined subjects, to lymphoid infiltration of the gland.

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Section: Discussionmentioning

confidence: 99%

Detection of human t lymphotropic virus type i tax gene in salivary gland epithelium from two patients with sjögren's syndrome

Mariette

Agbalika

Daniel

et al. 1993

Arthritis & Rheumatism

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“…After incubation for 1 h, the plates were washed with 0.1% Tween-20 in PBS. The plates were then further incubated with horseradish peroxidase-conjugated secondary antibodies, washed with 0.1% Tween-20 in PBS, and developed with 2,2'-azino-bis(2-ethylbenzthiazoline-6-sulfonic acid) as earlier described [25].…”

Section: Elisamentioning

confidence: 99%

Inhibition of the catalytic activity of human transaldolase by antibodies and site‐directed mutagenesis

Bánki

Perl

1996

FEBS Letters

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Transaldolase is a key enzyme of the pentose phosphate pathway. While antibody (Ab) 169, directed against the N-terminal 139 residues of human transaldolase (TAL-H), had no effect on enzyme activity, Ab 12484 raised against full length and functional recombinant TAL-H inhibited catalytic activity. This tentatively mapped the catalytic site to the C-terminal 140-336 amino acid portion of TAL-H. Dihydroxyacetone transfer reactions catalyzed by transaldolase depend on Schiff base formation by a iysine residue. Replacement of lysine-142 by glutamine using site-directed mutagenesis resulted in a complete loss of enzyme activity, suggesting that lysine-142 is essential for the catalytic activity of TAL-H.

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“…Interestingly, this region of CA shares limited sequence similarity with the SS-B autoantigen (23) (Figure 1). Banki et a1 (6) found that reactivities to peptides with this sequence were elevated in some SS patients and patients with other systemic autoimmune diseases, relative to that in healthy control subjects.…”

Section: Can Findings Of Htlv Tax Expression In Ss Patients Be Generamentioning

confidence: 95%

“…In addition to these studies, Banki and coworkers (6) suggested that, based on the results of serologic studies, an HTLV-related endogenous sequence (HRES-1) might be involved in SS and other systemic autoimmune diseases. Thus, comparisons of the sequences of HIAP and HRES-1 to sequences of the HTLV-related proviruses detected in some SS patients by Sumida et a1 and Mariette et a1 are warranted.…”

Section: What Is the Relationship Of These Findings To Studies Implicmentioning

confidence: 99%

New evidence for involvement of retroviruses in sjögren's syndrome and other autoimmune diseases

Garry¹

1994

Arthritis & Rheumatism

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Human T-cell lymphotropic virus (HTLV)-related endogenous sequence, HRES-1, encodes a 28-kDa protein: a possible autoantigen for HTLV-I gag-reactive autoantibodies.

Cited by 142 publications

References 35 publications

Detection of human t lymphotropic virus type i tax gene in salivary gland epithelium from two patients with sjögren's syndrome

Detection of human t lymphotropic virus type i tax gene in salivary gland epithelium from two patients with sjögren's syndrome

Inhibition of the catalytic activity of human transaldolase by antibodies and site‐directed mutagenesis

New evidence for involvement of retroviruses in sjögren's syndrome and other autoimmune diseases

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