Human T cell priming assay (hTCPA) for the identification of contact allergens based on naive T cells and DC – IFN-γ and TNF-α readout

Richter, Anne; Schmucker, Sonja; Esser, Philipp R.; Traska, Verena; Weber, Verena; Dietz, Lisa; Thierse, Hermann‐Josef; Pennino, Davide; Cavani, Andrea; Martin, Stefan F.

doi:10.1016/j.tiv.2012.08.007

Cited by 49 publications

(31 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The test is usually repeated on T cells derived from five different donors to minimize donorto-donor variability. T cells are isolated again and restimulated with autologous DC and the same control chemicals to assess antigen specificity [51]. Comparison of the proliferation during stimulation and re-stimulation is an important parameter.…”

Section: Important Gapsmentioning

confidence: 99%

See 1 more Smart Citation

In Vitro Approaches for Detection of Chemical Sensitization

Roggen

2014

Basic Clin Pharma Tox

View full text Add to dashboard Cite

Concerns, legislation and research needs have precipitated developments such as the mode of action concept, the Tox21 strategy, the concept of pathways of toxicity and the adverse outcome pathway framework. New technologies and paradigms are currently transforming these concepts into applicable animal-free toxicity testing systems. The adverse outcome pathway framework provides a structure for collecting, organizing and evaluating the available data that describe the compound and the events resulting in an adverse outcome at a biological level of organization. The current chapter intends to provide a nonexhaustive review of (i) our current understanding of the molecular mechanisms driven the key events of the mode of action for sensitization induction by chemicals, (ii) the tools that were developed on the basis of the available knowledge and (iii) the major gaps that need to be filled.

show abstract

Section: Important Gapsmentioning

confidence: 99%

“…Preliminary results demonstrated the capability of this assay to effectively predict antigenicity of chemicals, including drugs. Currently, two protocols [51,52] are optimized and harmonized and the potential to predict sensitization potency using frequency and T-cell receptor repertoire analysis is investigated.…”

Section: Important Gapsmentioning

confidence: 99%

In Vitro Approaches for Detection of Chemical Sensitization

Roggen

2014

Basic Clin Pharma Tox

View full text Add to dashboard Cite

show abstract

“…Proteomic-based technologies were applied for targeting chemical-specific human T-cell epitopes and for supporting quality control experiments in the establishment of a novel human T-cell priming assay (hTCPA) for the identification of contact allergens (Dietz et al, 2010;Martin et al, 2010;Richter et al, 2012). these technologies have provided new opportunities for the development of assays for tracking molecular and cellular allergen-protein interactions (thierse, et al, 2007), as well as for differential proteomic analyses in human skin cells activated by human allergens (Dietz et al, 2009).…”

Section: Overview Of the Cosmetics Europe (Formerly Colipa) Assay Devmentioning

confidence: 99%

“…Actually, instead of activating the iDC cells before, the test substance can be added directly to the co-culture system or after binding with a protein. As a followup step of the assay, t-cells are isolated again and re-stimulated with autologous DC and the same control chemicals to assess antigen-specificity (Martin et al, 2010;Dietz et al, 2010;Richter et al, 2012). Comparison of the proliferation during stimulation and re-stimulation is an important parameter.…”

Section: Immunomodulatory Properties Of Inhaled Chemicals (By Armin Bmentioning

confidence: 99%

“…Cosmetics europe is now funding a project aimed at the optimization and harmonization of the Sens-it-iv (Dietz et al, 2010;Richter et al, 2012) and lyon protocol (Vocanson et al, 2008) and its extension to predict sensitization potency using frequency and t-cell receptor repertoire analysis. the opportunity to apply the T-cell amplification method may go beyond the testing of sensitizers, making it the possible to predict immunogenicity, to assess immunocompetence, to monitor response to vaccination, and eventually to generate T-cells for cellular immunotherapy (Geiger et al, 2009).…”

Section: Immunomodulatory Properties Of Inhaled Chemicals (By Armin Bmentioning

confidence: 99%

See 1 more Smart Citation

Advanced tests for skin and respiratory sensitization assessment

Rovida

Martin

Vivier

et al. 2013

ALTEX

View full text Add to dashboard Cite

Denmark, aided by the vice-coordinator Hans-Ulrich Weltzien from the Max-Planck Institute for Immunobiology and epigenetics, Freiburg Germany. the ultimate goal of the Sens-it-iv project was the development of a set of in vitro methods for the assessment of the skin and respiratory sensitization potential of chemicals and proteins. the level of development was intended to be at the point to enter the pre-validation phase. After 66 months of work, it could be concluded that the goal was largely accomplished. Several advanced methods were evaluated extensively, and for some of them a detailed standard operating procedure (SOP) was established for pre-validation purposes. Other, less advanced methods contributed to our understanding of the mechanisms driving sensitization as well.Several opportunities were employed to assure dissemination of results. During the course of the project, 44 newsletters were released, presenting the main achievements of the project. Fly-1 Introduction

show abstract

Chemical Allergen‐Induced Skin Cell Activation

Martin

Esser

2014

Molecular Immunotoxicology

View full text Add to dashboard Cite

Human T cell priming assay (hTCPA) for the identification of contact allergens based on naive T cells and DC – IFN-γ and TNF-α readout

Cited by 49 publications

References 22 publications

In Vitro Approaches for Detection of Chemical Sensitization

In Vitro Approaches for Detection of Chemical Sensitization

Advanced tests for skin and respiratory sensitization assessment

Chemical Allergen‐Induced Skin Cell Activation

Contact Info

Product

Resources

About