Human T cell responses to Dengue and Zika virus infection compared to Dengue/Zika coinfection

Badolato-Corrêa, Jéssica; Sánchez‐Arcila, Juan Camilo; Souza, Thiara Manuele Alves de; Barbosa, Luciana Santos; Nunes, Priscila Conrado Guerra; Lima, Monique da Rocha Queiroz; Gandini, Mariana; Filippis, Ana María Bispo de; Cunha, Rivaldo Venâncio da; Azeredo, Elzinandes Leal de; de-Oliveira-Pinto, Luzia Maria

doi:10.1002/iid3.203

Cited by 36 publications

(45 citation statements)

References 47 publications

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“…Dengue virus‐specific T‐cells producing IFNγ and TNF can be found in human 9,29–31,37 and experimental 40 dengue infection. Interestingly, IFNγ + TNF + CD8 + T‐cells were also found to be increased in patients with a history of Hemorrhagic Dengue, 9 suggesting a deleterious role of these cells.…”

Section: Discussionmentioning

confidence: 99%

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T‐cells producing multiple combinations of IFNγ, TNF and IL10 are associated with mild forms of dengue infection

et al. 2020

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Multifunctional interleukin 10 (IL10) + Th1 cells have been implicated in favorable evolution of many infectious diseases, promoting an efficacious immune response while limiting immunopathology. Here, we investigated the presence of multifunctional CD4 + and CD8 + T-cells that expressed interferon gamma (IFNc), IL10 and tumor necrosis factor (TNF), or its combinations during dengue infection. Peripheral blood mononuclear cells (PBMCs) from outpatients with dengue (mild dengue forms) and hospitalized patients (or patients with dengue with warning signs and severe dengue) were cultured in the presence of envelope (ENV) or NS3 peptide libraries of DENV during critical (hospitalization period) and convalescence phases. The production of IFNc, IL10 and TNF by CD4 + and CD8 + T-cells was assessed by flow cytometry. Our data show that patients with mild dengue, when compared with patients with dengue with warning signs and severe dengue, presented higher frequencies of multifunctional T-cells like NS3-specific IFNc/IL10-producing CD4 + Tcells in critical phase and NS3-and ENV-specific CD8 + T-cells producing IFNc/IL10. In addition, NS3-specific CD8 + T-cells producing high levels of IFNc/TNF and IFNc/TNF/IL10 were also observed in the mild dengue group. We observed that multifunctional T-cells produced higher levels of cytokines as measured by intracellular content when compared with single producer T-cells. Importantly, multifunctional CD4 + and CD8 + T-cells producing IFNc, TNF and IL10 simultaneously displayed positive correlation with platelet levels, suggesting a protective role of this population. The presence of IL10 + Th1 and IL10 + Tc1 multifunctional cells was associated with mild dengue presentation, suggesting that these cells play a role in clinical evolution of dengue infection.

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Section: Discussionmentioning

confidence: 99%

“…There is little information about multifunctional T‐cells in dengue infection, 29–31 especially with regard to IL10 + Th1 and IL10 + Tc1 cells. In this work, we aimed to investigate the presence of these cells in different clinical forms of dengue infection.…”

Section: Introductionmentioning

confidence: 99%

T‐cells producing multiple combinations of IFNγ, TNF and IL10 are associated with mild forms of dengue infection

et al. 2020

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“…There is a hypothesis that the simultaneous presence of more than one flavivirus infection may potentiate negative sequelae. However, these studies are not definitive and need further exploration (Badolato-Correa et al 2018). Even in animal models, the results are still inclusive (Langerak et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Detection of Zika virus in paired urine and amniotic fluid samples from symptomatic and asymptomatic women and their babies during a disease outbreak: association with neurological symptoms in newborns

et al. 2019

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Paired maternal and newborn urine and amniotic fluid from 138 subjects collected during a Zika virus (ZIKV) outbreak was analyzed for ZIKV by gene amplification (RT-qPCR), and the findings were correlated with clinical symptoms and neurological anomalies in the babies. ZIKV was detected in 1 of 9 symptomatic women (11.1%) and in 19 of 129 asymptomatic women (14.7%). Neurological manifestations were present in 19 babies (13.7%), 10 of 20 (50%) positive and 9 of 119 (7.6%) negative (p < 0.001) for ZIKV. Twelve (8.6%) urines collected during gestation were ZIKV-positive; only 2 remained positive for ZIKV postpartum. Six (4.1%) newborn urines collected within 1 day of delivery were ZIKV-positive cases. In 3 of these cases, ZIKV was detected in mother's urine pre-and postpartum and in both mother's urine and babies' urine. Four of the amniotic fluid samples (2.9%) were ZIKV-positive. Among ZIKV-negative babies with neurological sequel, 87.5% were female; in contrast, 72.7% ZIKV-positive babies with neurological abnormalities were male (p = 0.019). We conclude that during a ZIKVoutbreak, clinical symptoms and ZIKV detection in biological fluids are poor predictors of infection and adverse neurologic sequel in newborns.

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“…Currently, one of the major public health and biomedical challenges for the region is represented by cocirculation and coinfection of different flaviviral and nonflaviviral arboviruses [1,[5][6][7][8][9]. Their differentiation as their cross-reactivity between flaviviruses, particularly, implies immunological and diagnostic problems, when used serological tests [10][11][12][13].…”

Section: Cocirculation and Coinfection Of Arbovirusesmentioning

confidence: 99%

“…In 2015, ZIKV emerged in LAC (although circulated apparently since 2013 in a silent pattern) as a leading public health priority, approximately 2 years after the CHIKV epidemic, leading to ZIKV, CHIKV, and DENV cocirculation, and the reporting of coinfections in different combinations. Although in the case of ZIKV infection, 80% of cases could be asymptomatic, clinical diagnosis of ZIKV, CHIKV, and DENV remains a challenge due to the considerable overlap in clinical presentations between them, and with other viral and nonviral infections [1,4,5], in addition also the problem of infection in patients with existing comorbidities that can lead to atypical, severe, and even fatal cases.…”

Section: Introductionmentioning

confidence: 99%

Cocirculation and Coinfection Associated to Zika Virus in the Americas

Sánchez‐Duque¹,

Rodríguez‐Morales²,

Trujillo³

et al. 2018

Current Topics in Zika

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Zika virus, a flavivirus, has arrived to Latin America in 2013. It became evident causing epidemics since 2015, first in Brazil and later in other countries in the region, such as Colombia, with a higher peak in 2016. The World Health Organization (WHO), based on cumulated evidence on its association with Guillain-Barre syndrome (GBS) and microcephaly and other birth defects (also the congenital Zika syndrome, CZS), declared for a period of almost a year, an international public health emergency. Epidemics in the region caused around 1 million cases with also additional complications beyond GBS and the CZS, which in patients with comorbidities lead to deaths. Among the events studied in the region, a number of cases with arboviral coinfections/codetection (dengue and chikungunya) were described and published beginning in Colombia and later in Brazil. In addition to that, cocirculation and still ongoing research on antibody-dependent enhancement (ADE) are challenges for physicians and public health authorities, given the implications for clinical manifestations and serological diagnosis in patients with previous exposition to other flaviviruses. We reviewed such aspects in this chapter.

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Human T cell responses to Dengue and Zika virus infection compared to Dengue/Zika coinfection

Cited by 36 publications

References 47 publications

T‐cells producing multiple combinations of IFNγ, TNF and IL10 are associated with mild forms of dengue infection

T‐cells producing multiple combinations of IFNγ, TNF and IL10 are associated with mild forms of dengue infection

Detection of Zika virus in paired urine and amniotic fluid samples from symptomatic and asymptomatic women and their babies during a disease outbreak: association with neurological symptoms in newborns

Cocirculation and Coinfection Associated to Zika Virus in the Americas

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