Human Tears: Electrophoretic Characteristics of Specific Proteins

Gachon, A.M.; Lambin, P.; Dastugue, B.

doi:10.1159/000265091

Cited by 36 publications

(6 citation statements)

References 9 publications

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“…The tear protein patterns of our subjects were visually compared with those reported by previous studies [13,21,27] and found to be not dissimilar. The six groups of major, well-known protein bands which have been reported by previous investigators were clearly separated from the tears of our subjects (see Fig.…”

Section: Tear Protein Patternsmentioning

confidence: 68%

Tear proteins of normal young Hong Kong Chinese

Ng¹,

Cho²,

To³

2000

Graefe's Archive for Clinical and Experimental Ophthalmology

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The results of total tear protein concentrations indicated that values obtained from different methods and different standards were not comparable. The tear protein patterns of our subjects were qualitatively similar to those reported for Caucasian subjects. However, the concentrations of the major proteins of our subjects were not in accordance with those reported previously. The main reason may be the large variability of method used.

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Section: Tear Protein Patternsmentioning

confidence: 68%

Tear proteins of normal young Hong Kong Chinese

Ng¹,

Cho²,

To³

2000

Graefe's Archive for Clinical and Experimental Ophthalmology

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“…2. The major tear proteins sIgA (observed as a smear on top of the gel), lactoferrin (seen as a double band), protein G (a dimer of TSPA of about 31 kD) (Gachon et al 1980;Kissner & Fullard 1991), TSPA (proteins migrating just above lysozmyme), and lysozyme are separated with good resolution. Furthermore, serum albumin can clearly be distinguished from the tear proteins (compare lane 4 with lanes 1-3).…”

Section: Sds-pagementioning

confidence: 99%

Analysis of tear fluid proteins in insulin‐dependent diabetes mellitus

Stolwijk

Kuizenga

Haeringen

et al. 1994

Acta Ophthalmologica

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Secretory immunoglobulin A, lactoferrin, lysozyme and tear specific pre-albumin were analyzed in stimulated tear fluid of 25 diabetic patients without retinopathy and in 29 diabetic patients with (pre) proliferative retinopathy using high performance liquid chromatography. Results were compared to those obtained in 26 healthy controls to determine the effect of diabetes mellitus on the exocrine function of the main lacrimal gland. Sodium dodecyl sulfate polyacrylamide gel electrophoresis onto minigels was performed on 20 tear samples for verification of high performance liquid chromatography fractions recorded. The mean total protein values in tear fluid (Bradford assay) of diabetics without retinopathy, with retinopathy and healthy controls did not differ significantly (mean in mg/ml +/- SD: 6.4 +/- 2.2, 5.9 +/- 2.0 and 5.7 +/- 1.7, respectively; Mann-Whitney; p > 0.02). High performance liquid chromatography showed an increased secretory immunoglobulin A and decreased peak 5 OD280 (+56% and -38%, respectively; p < 0.02) in patients without retinopathy, whereas in patients with retinopathy lysozyme was increased (+27%; p < 0.01) and tear specific pre-albumin and peak 5 OD280 decreased (-24% and -42%, respectively; p < 0.04), when compared to healthy controls. These inconsistent differences do not uniformly suggest an exocrine dysfunction of the main lacrimal gland in diabetic patients.

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“…TP 5 (18000/ 19000 D) has been called 'tear specific prealbumin' by Bonavida [11]. Gachon et al [12] demonstrated 6 proteins in this band and called them 'proteins migrating faster than albumin (PMFA)' TP 6 (16000 D) is lysozyme.…”

Section: Protein Patternmentioning

confidence: 99%

Tear ferning and other lacrimal tests in normal persons of different ages

Puderbach

Stolze

1991

Int Ophthalmol

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We investigated in normal persons of different ages ranging from 4 months to 85 years the frequency of different ferning types as well as the results of the Schirmer I test, break-up time and tear protein profile. With increasing age, ferning types of higher degree were significantly more frequent. Furthermore, a correlation was found between the ferning test and low Schirmer test values (p less than 0.05) as well as break-up time (p less than 0.1). No correlation exists between ferning the test and tear protein pattern. These results indicate that tear ferning is independent from single tear proteins but in correlation with secreted watery volume and plays some role in tear film stability. Furthermore, these normal values of ferning and protein pattern can be used as references for different ages.

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Human Tears: Electrophoretic Characteristics of Specific Proteins

Cited by 36 publications

References 9 publications

Tear proteins of normal young Hong Kong Chinese

Tear proteins of normal young Hong Kong Chinese

Analysis of tear fluid proteins in insulin‐dependent diabetes mellitus

Tear ferning and other lacrimal tests in normal persons of different ages

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