Human Tissue-Engineered Skeletal Muscle: A Tool for Metabolic Research

Kim, Ji-Hoon; Yu, Seung-Min; Son, Jang Won

doi:10.3803/enm.2022.302

Cited by 3 publications

(2 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An organoid may be basically defined as a miniaturized organ that can be established from human stem cells in vitro, including iPSCs, and studied at the microscopic level. Today, we are able to generate functional cell types or organoids for most organs involved in nutrient regulation or metabolic organs, including (but not limited to) the stomach (McCracken et al., 2014; Seidlitz et al., 2021), intestine (Günther et al., 2022), liver (Guan et al., 2021; Thompson & Takebe, 2020), adipose tissue (W. Hu & Lazar, 2022; Mandl et al., 2022), skeletal muscle (J. H. Kim et al., 2022), pancreas (Hirshorn et al., 2021; Jiang et al., 2022), brain (Agboola et al., 2021), and heart (Xuan et al., 2022; Figure 2).…”

Section: Nams For Nutrition Researchmentioning

confidence: 99%

Human‐based new approach methodologies to accelerate advances in nutrition research

Cassotta,

Cianciosi,

Elexpuru‐Zabaleta

et al. 2024

Food Frontiers

View full text Add to dashboard Cite

Much of nutrition research has been conventionally based on the use of simplistic in vitro systems or animal models, which have been extensively employed in an effort to better understand the relationships between diet and complex diseases as well as to evaluate food safety. Although these models have undeniably contributed to increase our mechanistic understanding of basic biological processes, they do not adequately model complex human physiopathological phenomena, creating concerns about the translatability to humans. During the last decade, extraordinary advancement in stem cell culturing, three‐dimensional cell cultures, sequencing technologies, and computer science has occurred, which has originated a wealth of novel human‐based and more physiologically relevant tools. These tools, also known as “new approach methodologies,” which comprise patient‐derived organoids, organs‐on‐chip, multi‐omics approach, along with computational models and analysis, represent innovative and exciting tools to forward nutrition research from a human‐biology‐oriented perspective. After considering some shortcomings of conventional in vitro and vivo approaches, here we describe the main novel available and emerging tools that are appropriate for designing a more human‐relevant nutrition research. Our aim is to encourage discussion on the opportunity to explore innovative paths in nutrition research and to promote a paradigm‐change toward a more human biology‐focused approach to better understand human nutritional pathophysiology, to evaluate novel food products, and to develop more effective targeted preventive or therapeutic strategies while helping in reducing the number and replacing animals employed in nutrition research.

show abstract

Section: Nams For Nutrition Researchmentioning

confidence: 99%

Human‐based new approach methodologies to accelerate advances in nutrition research

Cassotta,

Cianciosi,

Elexpuru‐Zabaleta

et al. 2024

Food Frontiers

View full text Add to dashboard Cite

show abstract

“…Each tissue type consists of cells and non-cellular structures that perform certain physiological functions (Mukund & Subramaniam, 2020). The structural elements of the cell are the membrane, the large nucleus, and the cytoplasm (Kim et al, 2022). The content of tissues in meat and its properties depend on the type of animal, its sex, age, fattening, and breed.…”

Section: Introductionmentioning

confidence: 99%

Key indicators of beef safety and quality as important aspects of conservation

Furman,

Sokulskyi,

Lisohurska

et al. 2024

Ukr. Jour. of Vet. and Agr. Sci.

View full text Add to dashboard Cite

Meat cattle breeding should develop in Ukraine as a priority and independent industry to meet beef needs. The meat industry has enormous potential and, as the most promising and attractive, will take a dominant place in the structure of the domestic food industry in the coming years. Beef is one of the most essential elements of the human diet. It contains complete proteins and animal fats, biologically active substances, mineral elements, and vitamins. The most important feature of food products is their quality and safety indicators, that is, the absence of substances harmful to the consumer's health and pathogens of infectious and invasive diseases. In this regard, veterinary and sanitary expertise and product safety control are of particular importance, as they are necessary to ensure the health of the population of Ukraine. Improving the quality of meat and meat products is a primary task in modern conditions. The implementation of this task depends not only on agricultural but also on processing enterprises. The article presents materials on the study of critical indicators of the safety and quality of beef. The study was carried out as part of the research work of the Department of Normal and Pathological Morphology, hygiene, and Expertise: “Monitoring the content of toxic substances in drinking water, feed and their impact on the body of cattle and the quality and safety of products”, state registration number – 0120U101318. The material for the research was the slaughter products of domestic animals, such as cattle, which were supplied to enterprises in the city of Zhytomyr. Pre-slaughter inspection and veterinary-sanitary examination of carcasses and their slaughter products of cattle was carried out following the “Rules of pre-slaughter veterinary examination of animals and veterinary-sanitary examination of meat and meat products”. The study aims to determine the safety and quality indicators of beef obtained in the conditions of meat processing enterprises of the Zhytomyr region. To achieve this goal, a comprehensive survey of beef safety and quality indicators was conducted. As a result of the tests, it was established that the pH level of the meat was 5.7–6.1. Carrying out the reaction with a 5 % solution of CuSO4 in the broth showed that the broth was transparent. A positive response to peroxidase was also detected. The safety parameters corresponded to regulatory documents. Careful control of the safety and quality of beef at every stage of production, starting from the rearing of animals and up to the sale of the product, guarantees a high degree of consumer protection.

show abstract

Myokines and Microbiota: New Perspectives in the Endocrine Muscle–Gut Axis

Saponaro,

Bertolini,

Baragatti

et al. 2024

Nutrients

View full text Add to dashboard Cite

This review explores the dual role of skeletal muscle as both a mechanical and endocrine organ, highlighting its contributions to overall health and its adaptability to various inputs such as nutrition, hormones, exercise, and injuries. In addition to its role in metabolism and energy conversion, skeletal muscle secretes signalling molecules called myokines (at rest) and exerkines (during/after physical exercise), which communicate with other organs like the brain, the cardiovascular system, and the immune system. Key molecules such as interleukins, irisin, and myostatin are discussed for their roles in mediating muscle health and inter-organ communication. This work also focuses on the muscle–gut axis, emphasising the bidirectional interaction between skeletal muscle and the gut microbiota, a complex ecosystem influencing immune defence, digestion, and metabolism. Muscle activity, particularly exercise, alters the gut microbial composition, promoting beneficial species, while gut-derived metabolites like short-chain fatty acids (SCFAs) impact muscle metabolism, mitochondrial function, and insulin sensitivity. Dysbiosis, or an imbalanced microbiota, can lead to muscle atrophy, inflammation, and metabolic dysfunction. This evidence highlights emerging research into myokines and exerkines as potential therapeutic targets for managing conditions like muscle decline, ageing, and metabolic diseases through muscle–gut interactions.

show abstract

Human Tissue-Engineered Skeletal Muscle: A Tool for Metabolic Research

Cited by 3 publications

References 39 publications

Human‐based new approach methodologies to accelerate advances in nutrition research

Human‐based new approach methodologies to accelerate advances in nutrition research

Key indicators of beef safety and quality as important aspects of conservation

Myokines and Microbiota: New Perspectives in the Endocrine Muscle–Gut Axis

Contact Info

Product

Resources

About