Human treated dentin matrices combined with Zn-doped, Mg-based bioceramic scaffolds and human dental pulp stem cells towards targeted dentin regeneration

Bakopoulou, Athina; Papachristou, Eleni; Bousnaki, Maria; Hadjichristou, Christina; Kontonasaki, Eleana; Theocharidou, Anna; Papadopoulou, Lambrini; Kantiranis, Nikolaos; Zachariadis, George A.; Leyhausen, Gabriele; Geurtsen, Werner; Koidis, Petros

doi:10.1016/j.dental.2016.05.013

Cited by 34 publications

(26 citation statements)

References 52 publications

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“…The GT biopsy designated for MSC culture (GT‐MSCs) and the analogous disease‐free specimen of gingival connective tissue (G‐MSCs) were enzymatically digested as described previously, seeded in 25 cm 2 flasks, expanded at 37°C in 5% CO 2 in alpha‐minimum essential medium (Life Technologies, Thermo Fisher Scientific, Paisley, UK) supplemented with 15% fetal bovine serum (Life Technologies), 100 units/mL of penicillin, 100 mg/mL of streptomycin and 0.25 mg/mL of amphotericin‐B (all from Life Technologies). GT‐MSCs and G‐MSCs were characterized by mesenchymal (CD90, CD73, CD146, CD271, STRO‐1), endothelial (CD105, CD106), hematopoietic (CD34, CD45) and embryonic (SSEA‐4) SC‐markers at passage 2 by flow cytometry, using a Guava ® easyCyte‐8HT Benchtop Flow Cytometer (Merck Millipore, Billerica, MA, USA) . A total of 50 000 events were acquired per sample.…”

Section: Methodsmentioning

confidence: 99%

Stem cell‐like populations and immunoregulatory molecules in periodontal granulation tissue

Apatzidou

Nile

Bakopoulou

et al. 2018

J of Periodontal Research

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Section: Methodsmentioning

confidence: 99%

Stem cell‐like populations and immunoregulatory molecules in periodontal granulation tissue

Apatzidou

Nile

Bakopoulou

et al. 2018

J of Periodontal Research

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“…Among the 52 papers, 11 have studied pure BGs, 23 have assessed doped‐glasses, and 18 have used composite materials …”

Section: Resultsmentioning

confidence: 99%

“…It can also be observed that by doping the glass, it is possible to improve cell response and to tailor the material to provoke a specific effect. For example, Cu was added to trigger angiogenesis, Li, or Sr to activate the wnt /β‐catenin canonical pathway, Zn was proved to play an active role in sustaining and promoting dental pulp regeneration, Mg seems to ensure a real improvement of cells proliferation especially when it is incorporated in bioscaffolds, Zr has high corrosion resistance and a remarkable ability for being bonded directly to the bone (a property called osteointegration) and providing biocompatibility and antiallergenic properties, and magnetic Fe was used to improve several cellular aspects such as adhesion, proliferation and differentiation.…”

Section: Discussionmentioning

confidence: 99%

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Sol–gel bioglasses in dental and periodontal regeneration: A systematic review

et al. 2018

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Due to their osteoconductive and osteoinductive abilities, bioglasses (BGs) have attracted attention in tissue engineering, especially for mineralized tissue. The aim of this study is to review the current state of the art on the effects of BGs produced by sol-gel on cells for dental and periodontal regeneration. The study also discusses associated antibacterial properties. The research was performed by considering the Preferred Reporting Items for Systematic Reviews and the Meta-Analyses (PRISMA) statement. The research ranged 5 years' window time (from January, 01, 2012, to August, 31, 2017) and the relevant studies were identified based on the inclusion/exclusion criteria. A total of 45 articles were selected from 244 initial returns, plus seven further articles coming from other sources were selected for the same purpose. From this systematic study, it is revealed that only 13 of the 52 articles have proved both the ability of BGs to differentiate dental cells at genetic level and their ability of triggering cell-mediated mineralization, but only six of them showed, along with cells, the antibacterial properties of the glasses. This review shows that sol-gel BGs are not toxic, can sustain cell proliferation and differentiation at a genetic level, and can keep the bacterial population under control. Moreover, a standard methodology and an ideal material are suggested. © 2018 Wiley Periodicals, Inc. J. Biomed. Mater. Res. Part B, 2018.

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“…Nevertheless, the utility of dentin‐based biomaterials in tooth root and bone regeneration has been demonstrated by independent laboratories (Bakopoulou et al, ; Kamal et al, ; Koga et al, ; Smith et al, ). In the future, it is expected that TDM will be used in a commercial biomaterial preparation, similar to demineralized bone matrix.…”

Section: Discussionmentioning

confidence: 99%

Proteomics and N‐glycoproteomics analysis of an extracellular matrix‐based scaffold‐human treated dentin matrix

Yang

et al. 2019

J Tissue Eng Regen Med

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Extracellular matrix (ECM)-based biomaterials developed from mammalian tissues have been successfully used in preclinical and clinical tissue engineering applications.We have previously reported about the applicability of dentin-based scaffold, treated dentin matrix (TDM), for tooth root regeneration. However, TDM protein composition has not been characterized. Here, we used a shotgun proteomic strategy to profile human TDM proteome. N-glycoproteins were enriched by lectin affinity chromatography and identified by mass spectrometry. The total human TDM proteome was compared with the previously published human dentin proteome, and bioinformatics analysis were performed accordingly. In total, 708 proteins were identified by mass spectrometry in human TDM, of which 208 were Nglycoproteins with 318 identified glycosylation sites. Collagens, proteoglycans, small integrin-binding ligand N-linked glycoproteins (SIBLINGs), and growth factors, such as COL1A1, biglycan, dentin sialoprotein, and transforming growth factor beta 1, were identified. Glycoproteins were enriched in "biological processes" Gene Ontology terms such as cellular process, biological regulation, response to stimulus, metabolic process, immune system process, and biological adhesion. Thus, our comprehensive study of the human TDM proteome revealed that dentin proteins are more heterogeneous than previously documented. Our findings provide clues for designing new biomaterials for tooth root regeneration and understanding dentin formation.

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Human treated dentin matrices combined with Zn-doped, Mg-based bioceramic scaffolds and human dental pulp stem cells towards targeted dentin regeneration

Cited by 34 publications

References 52 publications

Stem cell‐like populations and immunoregulatory molecules in periodontal granulation tissue

Stem cell‐like populations and immunoregulatory molecules in periodontal granulation tissue

Sol–gel bioglasses in dental and periodontal regeneration: A systematic review

Proteomics and N‐glycoproteomics analysis of an extracellular matrix‐based scaffold‐human treated dentin matrix

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