Human tumor cell destruction by distilled water anin Vitroevaluation

Mercill, Donald B.; Jones, Norman R.; Harbell, John W.

doi:10.1002/1097-0142(19850615)55:12<2779::aid-cncr2820551210>3.0.co;2-h

Cited by 17 publications

(5 citation statements)

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“…Peritoneal dissemination is considered to be caused by free peritoneal cancer cells exfoliated from serosally invasive tumors, and previous studies have reported the cytocidal effects of hypotonic stress on cancer cells [10–12]. We previously investigated changes in the cellular morphology and volume of gastric cancer cells subjected to hypotonic shock using several unique methods and apparatus [13].…”

Section: Discussionmentioning

confidence: 99%

“…Lin et al reported that peritoneal lavage with DW improved the survival rate of patients with spontaneously ruptured hepatocellular carcinoma [10]. Huguet and Keeling described the optimal method for peritoneal lavage with DW during colorectal cancer surgery [11], and Mercill et al found that exposure to distilled water reduced the number of surviving gastric cells [12]. In the present study, we intraperitoneally injected gastric cancer cells preincubated with DW into nude mice and analyzed the development of dissemination nodules in order to determine the efficacy of peritoneal lavage with DW during surgery.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, the roles of ion and water channels/transporters have recently been examined in cancer cells, and cellular physiological approaches are expected as novel therapeutic strategies [5–9]. The regulation of extracellular osmolality is a promising method, with previous studies demonstrating the cytocidal effects of hypotonic treatments on cancer cells [10–12]. We recently examined changes in the cellular morphology and volume of gastric cancer cells subjected to hypotonic shock using several unique methods and apparatus, such as a differential interference contrast microscope connected to a high-speed digital video camera and a high-resolution flow cytometer [13].…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of a Hypotonic Treatment for Peritoneal Dissemination from Gastric Cancer Cells: AnIn VivoEvaluation

Shiozaki

Ichikawa

Takemoto

et al. 2014

BioMed Research International

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The aim of the present study was to determine the efficacy of a hypotonic treatment for peritoneal dissemination from gastric cancer cells using an in vivo model. We firstly evaluated the toxicity of a peritoneal injection of distilled water (DW) (2 mL for 3 days) in mice. Macroscopic and microscopic examinations revealed that the peritoneal injection of DW did not severely damage the abdominal organs of these mice. MKN45 gastric cancer cells preincubated with NaCl buffer or DW for 20 minutes in vitro were then intraperitoneally injected into nude mice, and the development of dissemination nodules was analyzed. The total number, weight, and volume of the dissemination nodules were significantly decreased by the DW preincubation. We then determined whether the peritoneal injection of DW inhibited the establishment of peritoneal dissemination. After a peritoneal injection of MKN45 cells into nude mice, NaCl buffer or DW was injected into the abdominal cavity for 3 days. The total volume of dissemination nodules was significantly lower in DW-injected mice than in NaCl-injected mice. In conclusion, we demonstrated the safeness of a peritoneal injection of DW. Furthermore, the development of dissemination nodules from gastric cancer cells was prevented by a preincubation with or peritoneal injection of DW.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Efficacy of a Hypotonic Treatment for Peritoneal Dissemination from Gastric Cancer Cells: AnIn VivoEvaluation

Shiozaki

Ichikawa

Takemoto

et al. 2014

BioMed Research International

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“…The finding that murine melanoma , sarcoma , and 2 carcinomas were resistant to hypotonic shock was similar to results of in vitro studies of human malignant cell lines . 10 In vitro malignant mast cell death from hypotonicity occurred within 3 minutes . In our studies prior to the clinical injections , we determined that an intradermal and sc in-" jection of deionized water in the dog resulted in a hypoosmotic environment for at least 3 minutes .…”

Section: Discussionmentioning

confidence: 99%

Mast cell tumor destruction by deionized water

Grier,

Di Guardo,

Schaffer

et al. 1990

ajvr

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SUMMARY In a controlled study, malignant murine P815 mastocytoma cells exposed in vitro to distilled and deionized water died as a result of progressive swelling, degranulation, and membrane rupture. A 90% mean cell death occurred when cells obtained directly from culture were exposed to deionized water for 2 minutes. Of 6 cryopreserved malignant murine cell lines, which included Cloudman S91 melanoma, CMT-93 rectum carcinoma, MMT-06052 mammary carcinoma, and S-180 Sarcoma, only P815 mastocytoma and YAC-1 lymphoma were significantly (P < 0.05) affected by hypotonic shock; Cloudman S91 melanoma cells were the most resistant. Mastocytoma cells were selectively killed by hypotonic solution, and lymphoma cells were also killed by isotonic saline solution. Local mast cell tumor (mct) recurrence and percentage survival were evaluated in 12 cats (21 mct) and 54 dogs (85 mct) subjected to surgery alone or local infiltration of deionized water as an adjunct to surgery. Of all 16 incompletely excised mct in cats, there was no local recurrence following injection. Four mast cell tumors (2 cats) regressed after being injected in situ. In dogs with clinical stage-I mct, local recurrence was detected in 50% (5/10), but with injection after incomplete excision, local mct recurrence was significantly (P < 0.05) less (6.6%, 1/15). Percentage recurrence was significantly (P < 0.05) less and survival significantly greater when incompletely excised grade-II mct were injected. Mean follow-up period after surgery in cats and dogs was 35 and 23.4 months, respectively.

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“…Mercill et al observed a decrease in DNA synthesis in different tumour cell lines after exposure to distilled water for 1 to 10 min. However, the remaining cells did not lose their replication capacity [17]. In a study of Huguet et al, colorectal cancer cell lines were incubated with distilled water and no surviving cells were found beyond 12 min incubation.…”

Section: Possible Side Effectsmentioning

confidence: 99%

The use of distilled water in the achievement of local hemostasis during surgery

2009

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Distilled water is used worldwide to check on hemostasis at the end of pelvic oncological operations. Nevertheless, reports about this method are lacking. The aim of this study was to explain the method and to discuss possible side effects. After the addition of distilled water to the surgically exposed pelvis, rapid lysis of erythrocytes results in a transparent fluid in which a small source of bleeding is easily recognizable. A possible side effect of the lavage might be contribution to the formation of peritoneal adhesions by confusing the abdominal defence system. Systemic side effects are not to be expected. Although tumour cells might suffer from hypotonic distilled water lavage, the current use of distilled water at the end of surgery is probably not effective to lyse tumour cells. Our findings support the ongoing use of distilled water lavage to achieve hemostasis after extensive pelvic surgery.

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Human tumor cell destruction by distilled water anin Vitroevaluation

Cited by 17 publications

References 1 publication

Efficacy of a Hypotonic Treatment for Peritoneal Dissemination from Gastric Cancer Cells: AnIn VivoEvaluation

Efficacy of a Hypotonic Treatment for Peritoneal Dissemination from Gastric Cancer Cells: AnIn VivoEvaluation

Mast cell tumor destruction by deionized water

The use of distilled water in the achievement of local hemostasis during surgery

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